Catabolic Reaction - Crossref

In vitro reconstitution of the catabolic reactions catalyzed by PcaHG, PcaB, and PcaL: the protocatechuate branch of the β-ketoadipate pathway in Rhodococcus jostii RHA1

Abstract The u03b2-ketoadipate pathway in microbes is a key pathway involved in the synthesis of aromatic compounds. In this research, the protocatechuate branch of Rhodococcus jostii's u03b2-ketoadipate pathway was reconstituted in vitro. These reaction compounds, u03b2-ketoadipate enol-lactone, 3-carboxy-cis, muconolactone, and u03b2-ketoadipate, were further described using LC-MS and nuclear magnetic resonance; tu03b2-ketoadipate, muconate, 3-carboxymuconate, cis-muconate, u03b2-carboxymu03b2-ketoadipate enoadipate, 3-carboxymu03b2-carboxyl-lactone, mucono muconol-mu03b3-mu03b3-mu03b2-carboxymu03b3-mu03b2-carboxymu03b2-carboxymu03b3-mu03b2-carboxymu03b3-mu03b2-carboxymu03b2-carboxymu03b2-carboxymu03b3-mu In addition, the in vitro reaction of PcaL, a bidomain protein made up of u03b3-carboxy-muconolactone decarboxylase and u03b2-ketoadipate enol-lactone hydrolase enzyme enzymes, was shown for the first time.

Source link: https://doi.org/10.1080/09168451.2014.993915

Energetics of bacterial growth: balance of anabolic and catabolic reactions

Biomass formation is one of the most basic aspects of bacterial metabolism. For many years, it was believed that biomass production was proportional to the amount of ATP that could be obtained from energy-yielding pathways, but later research revealed that the ATP yield was not always a constant. Those bacteria were found to use ATP for metabolic reactions that were not directly related to growth, according to continuous-culture experiments. Maintenance energy seemed to be a growth rate-dependent function of the cell mass and time, according to mathematical derivations. Bacteria can release excess ATP in futile enzyme cycles, but there is no evidence that such cycles are widespread. Bacts can also dissipate ATP by diffusion of potassium, ammonia, and protons through the cell membrane, according to new research. It has been a curiosity to investigate the benefit of energy spilling in bacteria.

Source link: https://doi.org/10.1128/mr.59.1.48-62.1995

Polymerase chain reaction amplification of naphthalene-catabolic and 16S rRNA gene sequences from indigenous sediment bacteria

When subsurface sediment containing approximately 10 cells. g-1 was added directly to a PCR mixture, PCR amplification of indigenous bacterial 16S ribosomal DNA genes was ineffective. By PCR amplification, the nahAc naphthalene dioxygenase gene characteristic of the P. putida G7 NAH7 plasmid was identified by PCR amplification. The PCR amplification product's Southern blotting of samples inoculated with P. putida G7 improved sensitivity to 10 to 10 cells from samples, but controls with no sediment added indicated that the PCR was partially blocked by the sediments. When PCR conditions were changed by increasing Taq and primer concentrations, the nahAc gene could also be amplified from indigenous bacteria by using nahAc-specific primers. The nahAc amplification products from surface and subsurface sediments demonstrated polymorphism comparable to P. putida G7, according to restriction digests.

Source link: https://doi.org/10.1128/aem.59.3.687-694.1993

Kinetic parameters and relative turnovers of some important catabolic reactions in digesting sludge

The acetate-splitting units in well-digested sludge were about half saturated during natural steady-state conditions. Propionate-degrading plants were saturated to only 10% to 15%, and hydrogen removal was less than 1% of the maximum acceptable rate. A preliminary scheme of the substrate transport in sludge digestion is included. Acid formation coupled with hydrogen production via pyridine dinucleotide oxidation yields the immediate substrates, namely acetate and hydrogen, accounting for 54% of total methanogenesis.

Source link: https://doi.org/10.1128/aem.36.1.1-7.1978

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions