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Abstract The u03b2-ketoadipate pathway is a significant route in microbes' catabolism of the aromatic compounds. Genes from the u03b2-ketoadipate pathway have accumulated in the publicly available genetic database, contributing to a rapid accumulation of genes from the u03b2-ketoadipate pathway, yet the functions of these genes are unclear. In this research, the protocatechuate branch of Rhodococcus jostii's u03b2-ketoadipate pathway was restored in vitro. PcaHG, PcaB, and PcaL's reaction products were determined by high-performance liquid chromatography.
Source link: https://doi.org/10.1080/09168451.2014.993915
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