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Objective: We sought to determine the effects of fenofibrate on human cervical cancer cell cultures in the present study. Cell Counting Kit-8 determined the cytotoxicity of fenofibrate in cervical cancer cells. Results: We first showed that fenofibrate therapy improved cell viability in HeLa cervical cancer cells in a dose-dependent manner at 24 h and 48 h, providing importantly, apoptosis caused by cell cycle arrest in the G0–G1 phase and caspase-dependent apoptosis. In addition, fenofibrate-dependent autophagy stimulation triggered autophagy in a dose-dependent manner, and autophagy inhibition of autophagy resulted in an increase in sub-G1 phase and caspase-dependent cell death in HeLa cells. Conclusion: These results show that fenofibrate initiated cell cycle arrest in cervical cancer HeLa cells right away, followed by caspase-3-dependent cell death in cervical cancer HeLa cells. According, the combination of an autophagy inhibitor and fenofibrate may have the ability to develop a new therapeutic strategy for cervical cancer.
Source link: https://doi.org/10.1159/000518509
The primary aim of this research was to investigate the in vitro antiproliferative effects of isoalantolactone against liver cancer cells and monitor its mechanism of action. To determine the proapoptotic effects of isoalantolactone, proapoptotic reactions of isoalantolactone were used with western blotting and orange/ethidium bromide staining. Transwell migration and invasion of Hep-G2 cells were conducted to determine the effects of isoalantolactone on migration and invasion of Hep-G2 cells. In Hep-G2 cells, Western blotting was used to determine the expressions of Ras/Raf/MEK signalling pathway. The tested isoalantolactone molecule's IC50 value was found to be 71. 2 MHz and 53. 4 M at 12 h and 24 h time intervals respectively, according to the respective tables. While antiapoptotic Bcl-2 was reduced significantly, the proapoptotic effects of isoalantolactone were apparent from morphological studies and improved expressions of caspase-3, -8, and Bax, although proapoptotic Bcl-2 was reduced dramatically. These results also showed that isoalantolactone has a potent anti-proliferative activity against Hep-G2 liver cancer cells, according to their conclusion.
Source link: https://doi.org/10.18388/abp.2020_5704