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Cas9 Mediated Genome Editing - Springer Nature

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Last Updated: 26 July 2022

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Development of late-bolting plants by CRISPR/Cas9-mediated genome editing from mesophyll protoplasts of lettuce

Lettuce is a commonly eaten leafy vegetable crop. To delay flowering, one of the few molecular steps that may raise leaf yield of lettuce is to delay lettuce's emergence. Flowering is not limited to lettuce's use, but it is also a much-wanted phenotype for other leafy vegetable crops. We found that by triggering a single base mutation in SOC1 through the Cas9 protein-gRNA ribonucleoproteins pathway, the time to first flower bud sprouting in lettuce is longer than that of the wild variety. In addition, expression of the floral regulatory genes, including LsLFY, LsFUL, LsAPL1, and LsAPL2, was lower in the SOC1 gene edited plants than in the wild type. In addition, it is likely that direct RNP transfer from protoplasts can be used as a helpful tool for growing many gene edited crops.

Source link: https://doi.org/10.1007/s00299-022-02875-w

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions