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Clustered short palindromic repeats identified with the protein are an adaptive immune system of bacteria that can combat the imminent viral pathogen attack. We used the Cas9 endonuclease and dual 20 bp-gRNAs in two separate locations of stranded DNA-A of mungbean yellow mosaic virus, in greengram, to gain resistance in greengram in the latest study. The Agroinfiltrated T2 transgenic lines with minimal mosaic signs had minimal mosaic signs. In T2 modified lines, a drastic decrease in the accumulation of AC1 and AV1 was observed. Using the CRISPR/Cas9 system, you can obtain immunity to MYMV by editing the pathogen genome, which can be a cost-effective way to fight viruses and plant resistance in greengram.
Source link: https://doi.org/10.3389/fsufs.2022.911574
Abstract Background Genome editing is crucial for crop molecular breeding. However, gene editing in turnip has not been disclosed due to the very poor transformation results. The BrrWUSa transgenic plants, which were estrogen-inducible, were fertile and showed no obvious developmental defects. Conclusion The results show that BrrWUSA improves turnip regeneration rates. In turnip, the transformation process is a promising step to increase genetic transformation and functional characterization of genes.
Source link: https://doi.org/10.1186/s13007-022-00931-w