* If you want to update the article please login/register
Here we demonstrate that increasing chromatin open status is a key determinant of Cas9 editing activity in mammalian cells, and that increasing chromatin availability can greatly improve Cas9 genome editing efficiency. However, the Cas9's VP64 transcriptional activation domain only helps to boost genome editing activity slightly at the majority tested CRISPR/Cas9 targets, according to Lenti-X 293T cells. We demonstrate that using histone acetylation promoter YF-2 can promote genome editing from Cas9 and further from the Cas9 transcriptional activator.
Source link: https://www.ncbi.nlm.nih.gov/bioproject/827683
* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions