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Cas9 Protein - Europe PMC

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Last Updated: 10 September 2022

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Vitelline Membrane Protein 26 Mutagenesis, Using CRISPR/Cas9, Results in Egg Collapse in Plutella xylostella.

Vitelline membrane proteins are the key proteins that make up the inner shell of insect eggs and are an integral part of egg formation and embryogenesis. PxVMP26 was specifically expressed in female adults and was highly expressed in the ovary, according to both qPCR and western blot studies, and was highly present in the ovary. The expression level of PxVMP26 in the ovarian tube with an incomplete yolk was significantly higher than that in the ovarian tube with a complete yolk, according to a further anatomical analysis. The number of eggs laid within three days between wild and mutant individuals was found to be unchanged, but a lower egg hatchability was found. This first review of the role of the VMP gene in P. xylostella's oocyte formation and embryonic development of P. xylostella's P. xylostella provides a basis for testing new genetic control targets of P. xylostella.

Source link: https://europepmc.org/article/MED/36076934

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* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions