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Cas9 Mediated Genome Editing - Europe PMC

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Last Updated: 26 November 2022

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Highly Efficient CRISPR/Cas9-Mediated Genome Editing in Human Pluripotent Stem Cells.

Human pluripotent stem cells have a tremendous promise for both basic biology and cell-based therapies for a variety of conditions. For the non-targeted allele of a target's broken double-strand break, CRISPR/Cas9's high success in delivering targeted double-strand breaks, a significant challenge has been the introduction of precise genetic modifications on one allele without indel formation on the non-targeted allele. Basic Protocol 1: Application and optimization of CRISPR-based genome editing in human pluripotent stem cells Basic Protocol 2: CRISPR-based genome editing Basic Protocol 1: Utilization and optimization of CRISPR-based genome editing using a double-oligonucleotide CRISPR/Cas9 recombination technique.

Source link: https://europepmc.org/article/MED/36426905

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions