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Human pluripotent stem cells have a tremendous promise for both basic biology and cell-based therapies for a variety of conditions. For the non-targeted allele of a target's broken double-strand break, CRISPR/Cas9's high success in delivering targeted double-strand breaks, a significant challenge has been the introduction of precise genetic modifications on one allele without indel formation on the non-targeted allele. Basic Protocol 1: Application and optimization of CRISPR-based genome editing in human pluripotent stem cells Basic Protocol 2: CRISPR-based genome editing Basic Protocol 1: Utilization and optimization of CRISPR-based genome editing using a double-oligonucleotide CRISPR/Cas9 recombination technique.
Source link: https://europepmc.org/article/MED/36426905
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