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Cas9 Mediated Genome Editing - DOAJ

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Last Updated: 26 November 2022

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Targeted mutagenesis of the CYP79D1 gene via CRISPR/Cas9-mediated genome editing results in lower levels of cyanide in cassava

Cassava is the world's most important food root crop, providing calories to millions of subsistence farmers in Sub-Saharan Africa. Due to the conversion of the cyanogens to cyanide in the body, consumetination of residual cyanogens results in cyanide poisoning. In order to make it a consistently safe and acceptable food, as well as a commercial crop, there is a demand for acyanogenic cassava cultivars. Both the tested putative transgenic plants contained mutations within the MeCYP79D1 locus, as shown by deletions and substitutions upstream and downstream of the PAM sequence, respectively. The amounts of linamarin and evolved cyanide present in the leaves of mecyp79d1 lines' leaves had been reduced by up to sevenfold. However, the cassava linamarin and cyanide were not entirely eliminated by the MeCYP79D1 knockout, although the cassava linamarin and cyanide were not completely eradicated by the MeCYP79D1 knockout. Our findings show that CRISPR/Cas9-mediated mutagenesis is as an alternative route for plantation of cassava plants with reduced cyanide content.

Source link: https://doi.org/10.3389/fpls.2022.1009860

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions