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Cas9 - BioRxiv

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Last Updated: 10 August 2022

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Structural basis for Cas9 off-target activity

The CRISPR-associated genome editor nuclease Cas9's target DNA specificity is determined by complementarity to a 20-nucleotide segment in its guide RNA. Here we report crystallographic profiles of Cas9 bound to bona fide off-target substrates, revealing that off-target binding is facilitated by a variety of non-canonical base pairing interactions within the guide-off-target heteroduplex. Base skipping or multiple non-canonical base pairs are preferred over RNA bulge formation for off-target sites with single-nucleotide deletions relative to the guide RNA.

Source link: https://doi.org/10.1101/2021.11.18.469088

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions