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This study refined the crude polysaccharides of O. cartilaginous cells by macroporous resin absorption and optimized the purification process in the experiment of orthogonal design with four factors and three degrees, finding improved antibacterial and anti-cancer activity of the purified polysaccharides. OTP reduced the cell vibilities of the tested human cancer cells, i. e. , AGS, HCT116, HepG2, and Hela, in the anti-cancer study, and Hela exhibited the greatest inhibitory effect on HCT116. The expression of apoptosis-related proteins, cytochrome c, caspase 9 and caspase 3 increased in HCT116 apoptosis and affected the expression of apoptosis-related proteins, i. e. , affecting apoptosis-related proteins, cytochrome c, caspase 9 and caspase 3 increased, respectively. According to the present study, O. cartilaginous cell cultures have a potential use in food or drug manufacturing.
Source link: https://europepmc.org/article/MED/36328963
This review quantified the effect of subthreshold loading histories on the post-loading Ultimate Compressive Tolerance, yield capacity, and regional Cartilaginous End Plate indentation responses based on joint position, peak loading variation, and loading duration. Following conditioning and cyclic compression exposures, spinal cords were disected, and one endplate from each vertebra's vertebra underwent additional UCT or microindentation testing. UCT testing was carried out by compressing a single vertebra at a rate of 3 kN/s using an indenter made to a standard intervertebral disc size and shape. Indentation was carried out at five surface locations using a Motoman robot and aluminum indenter. The UCT response was simulated by the indentation stiffness in the central CEP. These results show a dramatic effect of posture on post-loading UCT and CEP behavior.
Source link: https://europepmc.org/article/MED/36327666
Bone fractures are one of the most common surgical large-organ injuries, and although some fractures can heal on their own, 2-12% of fractures are slow to heal or do not heal. Autologous grafts are now being used for nonunion recovery, but are not characterized with abundant bone tissue. It was previously reported that cartilaginous microspheroids of periosteum-derived cells could be assembled into scaffold-free constructs and repair murine moderately long bone fractures. Periosteum-derived microspheroids were cultured on MEW meshes and gene expression analysis revealed up-regulation of chondrogenic and prehypertrophic gene markers after 14 days, as well as a biohybrid sheet. However, bone formation was also observed when implanted in a murine critically-sized long bone defect, despite a significant difference between samples being revealed. The flexibility of this biofabrication approach lies in the ability to cut and dimensions appropriate to large bone defects and the individual patient using strong bone forming building blocks. Successful treatments for long bone injuries are still inadequate, and 2-12% of fractures do not heal properly. We combined a new biofabrication method, melt electrowriting, with robust biology: bone forming cartilaginous spheroids, to produce biohybrid sheets that are able to produce bone on implantation. "Biofabrication with Spheroid and Organoid Materials" is a rapidly growing field; the present research contributes to the growing field of "Biofabrication of Functional Tissues by the production of cellular spheroids.
Source link: https://europepmc.org/article/MED/36283613
Objective Cartilaginous endplate degeneration is one of the early signs of intervertebral disc degeneration, and it is closely related to oxidative stress. We wanted to see if the Nrf2 which was increased in expression by 4-octyl itaconate would prevent intervertebral disc degeneration by reducing macrophage-associated inflammation and cartilaginous endplate degradation. Methods Firstly, we established the presence of Nrf2 in human degenerative CEPs. In human degenerative CEPs, we found increased expression of Nrf2 in decrease. 4OI greatly reduced the progression of IVDD by MR images and histological examination, thanks in large part to a rat IVDD model. Immunofluorescence results show that 4OI therapy has decreased CEPs and macrophage-associated inflammation, which is suppressed. In addition, 4OI inactivated Nrf2-in LPS-induced rat CEP cells' ZNF598-dependent ubiquitination of Nrf2 in LPS-induced rat CEP cells. Conclusions 4OI can help with IVDD by reducing CEP degeneration and macrophage-associated inflammation. Degenerative CEPs/IVDs may be a viable treatment for 4OI.
Source link: https://europepmc.org/article/MED/36270478
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