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Cartilage Tissue Engineering - Europe PMC

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Last Updated: 25 January 2023

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Hyaluronic acid and multiwalled carbon nanotubes as bioink additives for cartilage tissue engineering.

We first characterized the minimal conditions that improve cell permeability and reduced reactive oxygen species and apoptosis in 2D cell cultures of human knee articular chondrocytes and human adipose-derived mesenchymal stem cells in 2D cell cultures of normal human knee articular chondrocytes and human adipose-derived mesenchymal stem cells in normal human knee articular chondrocytes Only a combination of variables positively influenced cell viability throughout the entire experiment, according to test results conducted on 3D bioprinted constructs. Gene expression analysis of COL1A1, COL6A1, HIF1A, COMP, RUNX2, and POU5F1 showed significant declines in the expression of all tested genes, with a gradual decrease in transcriptional activity in the majority of them.

Source link: https://europepmc.org/article/MED/36635477


Progress of Microfluidic Hydrogel-based Scaffolds and Organ-on-Chips for the Cartilage Tissue Engineering.

Cartilage degeneration is one of the key causes for disability and pain around the world. In this regard, the integration of tissue engineering and microfabrication principles may lead to the design of more sophisticated microfluidic devices, thus providing more cost-effective alternatives to current treatments and traditional constructs used in tissue engineering applications. The latest findings involving microfluidic hydrogel-based scaffolds, which include hydrogels with microfluidic channels to hydrogels prepared by the microfluidic machines that enable therapeutic delivery of cells, drugs, and growth factors, as well as cartilage-related organ-on-chips are reviewed herein. Different types of hydrogels are introduced, and the advantages of microfluidic hydrogel-based scaffolds and hydrogel-based microfluidic platforms over traditional hydrogel-based microfluidic platforms are explored. The preclinical and clinical uses of microfluidic hydrogel-based scaffolds in cartilage repair and the production of cartilage-related microfluidic chips over time are discussed.

Source link: https://europepmc.org/article/MED/36633376


Extracellular Vesicle Isolation and Characterization for Applications in Cartilage Tissue Engineering and Osteoarthritis Therapy.

Extracellular vesicles have the ability to be used in cartilage tissue engineering by inciting tissue repair and microenvironmental reprogramming. To attribute observed biological results to EVs as genuine effectors, it is necessary to isolate EVs isolated from different source materials such as conditioned cell culture media or biofluids. This chapter discusses a density- and a size-based procedure, as well as a combination of both for isolation of EVs from culture media or biofluids.

Source link: https://europepmc.org/article/MED/36355289


Injectable MSC Spheroid and Microgel Granular Composites for Engineering Cartilage Tissue

We reimagine cartilage tissue engineering by mixing aggregates of adult MSCs with hydrogel microparticles to create granular composites that are injectable, encourage cell-cell contact for chondrogenesis, promote spheroid formation, and even allow for interparticle crosslinking for stability, and allow for cell-cell contacts for proliferation and expansion. In granular composites that balance mechanical stability with tissue growth, we use simulations and experimental studies to determine the importance of initial MSC spheroid to microgel volume ratios.

Source link: https://europepmc.org/article/PPR/PPR590043


Cartilage biomechanics: From the basic facts to the challenges of tissue engineering.

Articular cartilage is the thin tissue that covers the long bone ends in the joints and that guarantees the transfer of forces between adjacent bones while also allowing virtually frictionless movement between them. AC repair has been a technological and scientific challenge that has been tackled with various approaches. Additional information regarding AC biomechanical properties remains unanswered, particularly the differences in extracellular matrix stiffness measured at the microscale and on the millimetric scale. However, AC repair plans must also take into account what are commonly considered the key mechanical characteristics of cartilage: its ability to withstand high strains through three main behaviors. Finally, we recommend that future studies investigate AC mechanical properties at various scales, particularly the difference between mechanical properties at different scales.

Source link: https://europepmc.org/article/MED/36583681

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions