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The prevention of osteoarthritis prevention by long-term treatment of articular cartilage injuries is critical, but it remains a big problem. Three research in 2022 pioneer new approaches to enhancing articular cartilage regeneration. In vivo, a synthetic stem cell system that is supposed to imitate mesenchymal stem cells attenuated cartilage degeneration and increases articular cartilage's biomechanical properties.
Source link: https://doi.org/10.1038/s41584-022-00889-y
Extracellular vesicles are able to be used in cartilage tissue engineering by stimulating tissue regeneration and microenvironmental reprogramming. To attribute observed biological reactions to EVs as genuine effectors, it is vital to isolate EVs isolated from various source materials, such as conditioned cell culture media or biofluids. This chapter discusses a density- and a size-based method, as well as a combination of both for isolation of EVs from conditioned cell culture media or biofluids.
Source link: https://doi.org/10.1007/978-1-0716-2839-3_10
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