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In the current research, we investigated the repair potential of stem cell microcarrier construct in cartilage defect models. A total of 39 healthy New Zealand white rabbits were included in the pack, as well as femoral trochlear cartilage defect models. On days 4, 8, and 30, following transplantation, Stem cell microcarrier constructs were implanted into cartilage defects, and cartilage defects were detected, and the implanted constructs' maintenance conditions were recorded. The reconstitution of cartilage defects at 12 weeks post-cartilage defect repair was based on gross observation and pathological investigation. The microcarriers may be able to fill the defect model with high plasticity and mesh well with the boundary native normal cartilage. In the microcarrier without stem cell culture group, there were fibrous cartilage tissues filled with fibrous cartilage tissue in the 3 months after implantation. These findings demonstrate the feasibility of stem cell microcarrier technology in treating cartilage defects, indicating promising clinical application prospects.
Source link: https://europepmc.org/article/MED/35900693
Although articular cartilage lacks intrinsic reparative ability, numerous studies have reported the existence of cartilage-resident stem/progenitor cells in the superficial zone of articular cartilage. We will explore CSPCs' contribution in cartilage development, homeostasis, and repair, as well as the application of CSPCs in cartilage repair and OA therapies in this report.
Source link: https://europepmc.org/article/MED/35892602
A review of cartilage regeneration has shown that mesenchymal stem cell-derived extracellular vesicles are important for cartilage regeneration. MSC-EVs carrying microRNA-181c-5p in cartilage repair Herein, we investigated the role of human umbilical cord MSC-EVs carrying microRNA-181c-5p in cartilage repair. In addition, miR-181c-5p can target and inhibit SMAD7 expression to promote bone morphogenic protein 2-induced proliferative, migratory, and chondrogenic differentiation potentials of BMSCs. The repairing effect of hUCMSC-EVs carrying miR-181c-5p on cartilage injury was highlighted in this article.
Source link: https://europepmc.org/article/MED/35782228
Cartilage is a connective tissue that outlines the body's organ structure and is made up of chondrocytes that produce a rich extracellular matrix and a variety of ground substances, such as proteoglycan and elastin fibers. In particular, 3D spheroid culture methods can increase the yield of exosome stem cells in comparison to two-dimensional culture, as well as boost cell function by raising MSCs' patriotism. Information exchange influences recipient cell phenotypes. We reviewed recent advances in 3D culture techniques, cartilage regeneration with stem cells, the effects of exosomes on chondrogenic differentiation, and chondrogenic-specific metrics related to stem cell derived exosomes in this article. If more mechanistic experiments are carried out at the molecular level, MSC-spheroid-derived exosomes could be a more effective therapeutic solution to osteoarthritis.
Source link: https://europepmc.org/article/MED/35888029
Background Icariin has long been used in osteoporosis treatment. Methods: The first, rabbit bone marrow mesenchymal stem cells were isolated, cultured, and identified. In addition, ICA promoted the expression of type II collagen, aggrecan, and bone morphogenetic protein 2 in BMSCs, while the BMP-Smad inhibitor reversed the repair effect of ICA on BMSCs. Our results indicated that the ICRS score of the BMSC and ICA treatment group was higher than In vivo. Moreover, BMSC and ICA therapy promoted chondrocyte formation and repaired cartilage-like tissue on the surface of cartilage defect. Conclusions The combined application of ICA and BMSCs will heal rabbit knee cartilage damage by governing the BMP/Smads pathway, indicating that ICA and BMSCs can be a cost-effective clinical therapy approach for knee cartilage damage in knee cartilage injuries.
Source link: https://europepmc.org/article/MED/35845525
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