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This PEG-CHI-KGN DN hydrogel possesses desirable architectures, useful mechanics, remarkable cellular affinity, and sustained KGN release, which may be able to promote peripheral blood-derived mesenchymal stem cells differentiation and extracellular matrix secretion. The allogeneic PB-MSCs were discovered to live for just 3 months in the regenerated cartilage after tracing the transplanted cells by analyzing the rabbit sex-determining region Y-linked gene sequence. KGN's long-term release of KGN is able to stimulate multiple genes and signaling pathways in order to promote chondrogenesis, chondrocyte differentiation, and PB-MSC survival.
Source link: https://europepmc.org/article/MED/36253092
Ste cell therapy has steadily sparked increasing interest in the treatment of OA. Cartilage stem/progenitor cells, a form of stem cell culture found on the surface of articular cartilage's surface, have several similarities to mesenchymal stem cells. In combination with the study's progress, we will give an overview of the cellular characteristics of CSPCs and their role in OA. Despite some existing limitations, CSPCs also have an innovative suggestion for OA treatment with great benefits.
Source link: https://europepmc.org/article/MED/36201278
Mesenchymal stem cells have demonstrated chondroprotective activity in cartilage repair. We created EXOs from human bone marrow mesenchymal stem cells and explored their therapeutic capabilities in cartilage repair in the present study. The treatment of hBMSC-EXOs in primary chondrocytes has markedly raised cell viability and proliferation in a dose-dependent manner. Moreover, wound healing assay results showed that hBMSC-EXOs promote cell migration in primary chondrocytes. The mitochondrial membrane potential was boosted by hBMSC-EXOs, according to JC-1 staining, cell apoptosis was reduced in the presence of hBMSC-EXOs. Staining of rabbits with articular cartilage defects, local government with hBMSC-EXOs supports cartilage regeneration as shown by gross view and hematoxylin-eosin. Overall, our results show that the hBMSC-EXOs therapy is a safe cell-free therapy for cartilage defects, and that these results are likely due to increased cell proliferation and migration in chondrocytes.
Source link: https://europepmc.org/article/MED/36117721
BACKGROUND: Certain conditions can cause stem cells to produce mysterious hormones that differ from each condition, such as hypoxia. Mesenchymal Stem Cells from Umbilical Cord-derived Mesenchymal Stem Cells have been shown to have higher proliferation rates, plasticity, and greater self-improvement ability than MSCs from other sources. A proteomic analysis of CM-UCMSCs that remained in a state of fasting was performed using SDS PAGE and electrospray ionization mass spectrometry using mass spectrometer. We found one strong band, but we used Mass Spectrometry to continue Mascot sequencing protein that band, and we discovered that the protein was a D4A9T1/ RNF-111/ Arkadia Protein. The chondrogenesis was induced by Arkadia Protein's synergistically with TGF-21 Protein, according to a reviewer who used a silico-based docking technique. Nevertheless, further study is required before the clinical application of CM-UCMSCs in cartilage repair is feasible and safe.
Source link: https://europepmc.org/article/PPR/PPR534991
The aim of the investigation was to determine the benefits of without enzyme isolating patellar fat pad-derived mesenchymal stem cells and cartilage repair. Twenty-four New Zealand rabbits were randomly divided into four groups. The rabbits in group A were given the mixture of IPFP-SCs and pure PRP, separated by the enzyme method, while others in the group B were treated with the IPFP-SCs and PRP compound, some in the group B were administered with the same IPFP-SCs and PRP separated with the separation procedure, while others in the group B were injected with the IPFP-SCs and PRP separated by the enzyme method, while others in group B were administered with the same IPFP-injected with the PRP injected with the PRP were injected with the PRP injected with pure PRP isolated using the PRP, while others in the PRP treated using the PRP, and PRP injected with the PRP separated using the PRP treated with the PRP diluted using the PRP isolated using the PRP treated with the PRP injected with the PRP sacetate technique, and PRP injected with PRP separated using the without enzyme technique, some in the PRP isolated using the without enzyme technique, some of SVF-PRP-PRP separated with the without enzyme technique were salined's The cartilage repair of rabbit joint specimens was observed and evaluated by gross observation and histological staining, and the effects of different IPFP-SCs application methods in treating cartilage defects were compared between six weeks and 12 weeks after surgery. Conclusion: The repair activity in group C was less costly than that in the previous two groups, but it was still better than that in group D, but it was still better than the one in group A. Conclusion: While the cell identification and differentiation potential between the two methods is similar, there is no significant difference in cell identification and differentiation potential between the two methods. The rabbit cartilage defect model had a good repair success, giving tips and reference for the clinical use of stem cells in reconstructing articular cartilage.
Source link: https://europepmc.org/article/MED/36061444
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