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While early detection and repair of cartilage lesions are crucial in osteoarthritis therapy, it is also difficult because neither clinically used drugs nor magnetic resonance contrast agents can be able to detect and repair simultaneously. The WY-CMC-MnOu2093 NPs significantly enhanced the MR imaging quality of cartilage lesions relative to non-cartilage-targeting NPs, owing to their tiny size and cartilage-targeting capabilities. In addition, WY-CMC-MnOu2093 stimulated chondrogenesis in mesenchymal stem cells, thus increasing OA therapy by effective cartilage regeneration after intraarticularly injection in destabilizing medial meniscus rat models.
Source link: https://europepmc.org/article/MED/IND607820939
3D bioprinting is a key area of interest in health sciences for specialized manufacturing, but there are no bioinks to improve the shape fidelity of 3D bioprinting and tissue repair for specific clinical uses. According to a rabbit cartilage defect model, the new naringin-derived medical bioink significantly improves cartilage defect repair's effectiveness and quality, indicating that the bioink is suitable for cartilage defect repair applications and a viable option is provided for the manufacture of medical bioinks for specific clinical uses.
Source link: https://europepmc.org/article/MED/36043510
In 1977, the first attempt to repair cartilage using tissue engineering was announced. Since then, cell-based therapies in orthopaedics have flourished, and several tissue engineering techniques for cartilage repair are in the translational pipeline toward clinical application. For the design of cartilage structures, we'll explore the benefits and drawbacks of using various cell types, natural and synthetic scaffolds, multiphasic or gradient-based scaffolds, or self-assemble scaffold-free scaffolds. Understanding the underlying mechanisms of cell therapy and tissue engineering approaches' success will help improve and refine therapy development. We continue to discuss recent findings of cartilage repair's intrinsic cellular and molecular mechanisms, which have revealed novel signals and goals and sparked the development of molecular therapies to enhance joint-resident stem and progenitor cell recruitment and cartilage reparative activity.
Source link: https://europepmc.org/article/MED/36150678
PEG-GelMA [Poly Dimethacrylate-Gelatin Methacrylate, PGMs] hydrogel microgel-based curcumin delivery system for both enhanced anti-inflammatory and pro-regenerative results in cartilage defects therapy. In vitro, this PGMs embedding curcumin in a relatively small dose was shown to promote proliferation and chondrogenic differentiation of mesenchymal stem cells. Under IL-1u03b2 stimulation, PGMs were shown to attenuate the inflammatory response of chondrocytes. These results showed that curcumin-loaded PGMs would not only improve the chondroprotective capacity under inflammatory conditions, but also promote effective cartilage repair.
Source link: https://europepmc.org/article/MED/36177180
GelMA-AG shows an increase in both the mitochondrial membrane potential and the production of intracellular adenosine triphosphate, as well as the reduction of cartilage tissue inflammation, leading to the increased level of cellular metabolism and tissue inflammation. The Er value of the newly restored rabbit knee articular cartilage is up to 70. 14 MPa, which is more similar to natural cartilage, in comparison to GelMA. This approach does not include exogenous cells and growth factors, and the therapeutic goal of specifically controlling the metabolic microenvironment of cartilage repair via a silicone rubber scaffold represents a novel and prospective approach for tissue engineering biomaterials in cartilage repair with simplification and success.
Source link: https://europepmc.org/article/MED/36089087
Mesenchymal stem cells have demonstrated chondroprotective activity in cartilage repair. We syntherated EXOs from human bone marrow stem cells in the present study and investigated their therapeutic potentials in cartilage repair. The administration of hBMSC-EXOs in primary chondrocytes significantly improves cell viability and proliferation in a dose-dependent manner. In addition, wound healing assay showed that hBMSC-EXOs also triggered cell migration in primary chondrocytes. The mitochondrial membrane potential was raised by hBMSC-EXOs, according to a JC-1 staining, indicating cell apoptosis was reduced in the presence of hBMSC-EXOs. As shown by gross view and hematoxylin-eosin staining, rabbits with articular cartilage defects, the local government with hBMSC-EXOs supports cartilage repair. Overall, our results show that the therapy with hBMSC-EXOs is a safe cell-free therapy for cartilage defects, and that this is likely due to increased cell proliferation and migration in chondrocytes.
Source link: https://europepmc.org/article/MED/36117721
In patients with medial meniscus posterior root tear ulceration, patients with transtibial pullout repair yields improved medical outcomes. However, the association between repaired meniscal root healing status and postoperative clinical outcomes remains unclear. After a failure with two simple stitches, we wanted to see changes in articular cartilage damage and clinical scores. Methods Thirty-three patients who underwent TSS pull out repair were analyzed. M medial extrusion and cartilage damage were assessed preoperatively and 1 year postoperatively, according to the MM medial extrusion and cartilage damage. The Knee Injury and Osteoarthritis Outcome score, Lysholm's score, International Knee Documentation Committee scores, and a graphic analogue scale pain score were used to analyze medical findings. At 1 year postoperatively, no quantitative differences were found in cartilage damage between the medial femoral condyle and the medial plateau area. Conclusion: TSS repair, stability of repaired meniscal root negatively related to cartilage damage in the medial compartment loading area.
Source link: https://europepmc.org/article/MED/36089432
This paper discusses two cases of late cartilage delamination in two young adults following two separate autologous cell-based therapies for cartilage repair: 1. It's likely that regardless of the technique used or the timeframe since the surgery, a graft failure may have occurred at some level, causing delamination of a previously asymptomatic cartilage repair graft and a painful event with long-term follow-up.
Source link: https://europepmc.org/article/MED/36069954
Herein, we developed cartilage-targeting hydrogel microspheres with reactive oxygen species sensitivity capability by combining the microfluidic method and photopolymerization techniques to incorporate cartilage-targeting peptides and ROS-responsive nanoparticles in the hydrogel matrix, inspired by the fact that bees track the scent of flowers to collect nectar. The hydrogel microspheres with cartilage-targeting capabilities improved stability in the joint cavity and increased cellular uptake of the nanoparticles. In addition, the ROS-responsive nanoparticles may interact with osteoarthritis-induced intracellular ROS, resulting in the depolymerization of nanoparticles, which may not only reduce excess ROS and inflammation, but also promote the release of dexamethasone and kartogenin in situ, facilitating safe OA therapy. In addition, the hydrogel microspheres, which look like bees, could be used to target and effectively repair cartilage in the OA model. Hence, injectable hydrogel microspheres had a great ability to restore OA and could also serve as an effective route for inflammatory bowel disease therapy.
Source link: https://europepmc.org/article/MED/36063108
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