* If you want to update the article please login/register
We produced 3D printed PCL scaffolds embedded with polylactic acid microspheres embedded with decellularized cartilage matrix decellularized cartilage matrix, which contained decellularized cartilage matrix. Human mesenchymal stem cells seeded on the composite scaffolds with DM and cultured in basal media self-assembled into aggregates that mimicking mesenchymal condensates during embryonic development. Moreover, the DM composite scaffolds induced higher levels of biochemical markers of cartilage growth than controls, providing more evidence for their translational use in the treatment of OCIs. The present research reveals the possibility of direct incorporation of DM with thermoplastics for 3D printing of patient-specific scaffolds for osteochondral regeneration.
Source link: https://europepmc.org/article/MED/35930819
Microfracture is the most commonly used surgical treatment for cartilage defects. We're hoping that a microenvironmentally optimized scaffold that regulates mesenchymal stem cell fate would be a good therapeutic option for cartilage repair. The microfracture not only caused inflammatory cell aggregation in blood clots, but also consisted of loose granulation tissue with elevated granulation tissue protein levels, which are related to fibrogenesis. To modify mesenchymal stem cell fate, we developed a functional cartilage scaffold using two strong bioactive cues, transforming growth factor-u03b23, and decellularized cartilage extracellular matrix. Microenvironmentally optimized scaffolds had positive results on modulating the mesenchymal stem cell fate, such as encouraging cell migration, proliferation, and chondrogenesis, according to in vitro studies. In summary, this report shows that microenvironmentally optimized scaffolds aid cartilage regeneration in situ by regulating the microenvironment and promoting further translation of human cartilage repair. More importantly, the adverse effects of MF therapy have prompted scientists to honed on tissue engineering scaffolds that may have sufficient therapeutic value. TGF-u03b23 scaffold scaffolds' continuous release of TGF-u03b23 from scaffolds has stimulated endogenous stem cell proliferation and differentiation, which led to tissue differentiation and differentiation. This microenvironmentally optimised scaffold resulted in improved tissue repair success in the sheep model, essentially guiding more organized neotissue formation and, in turn recapitulating native articular cartilage's anisotropic structure. We speculated that the cell-free scaffolds might increase the clinical applicability and become a new therapeutic option for chondral defect repair.
Source link: https://europepmc.org/article/MED/35896136
The new surgical standard of care, including microfracture, is the most recent surgical technique; however, MFX produces inferior fibro-cartilaginous tissue, which provides only temporary symptomatic relief. Here we transplanted solubilized articular cartilage extracellular matrix scaffolds into cadar defects in goats, effectively anchoring the implants to the joint surface using a 3D-printed fixation system that eliminated the need for sutures or glues. In a clinically relevant large animal model, this research shows that ECM-derived biomaterials, either alone or in combination with exogenous growth factors, can effectively address articular cartilage defects.
Source link: https://europepmc.org/article/MED/35865410
* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions