Advanced searches left 3/3

Cartilage Regeneration - Europe PMC

Summarized by Plex Scholar
Last Updated: 02 November 2022

* If you want to update the article please login/register

A Cell-free Silk Fibroin Biomaterial Strategy Promotes in situ Cartilage Regeneration Via Programmed Releases of Bioactive Molecules.

In situ tissue repair by constructing cell-free bio-functional scaffolds has been extensively investigated as a promising alternative therapy for cartilage repair. The first transplant growth factor-u03b21 was initially loaded onto the SF scaffolds by physical absorption and later functionalized with bone marrow stem cells -specific affinity peptide by gradient degradation coating of Silk fibroin Methacryloyl/Hyaluronic acid Methacryloyl. Finally, in vivo studies, the introduction of the bio-functional scaffold enhanced the recruitment of BMSCs to the implanted sites and further in situ cartilage repair in rabbit cartilage defects. This cartilage-biomimetic bio-functional scaffold with sequential controlled release of E7 and TGF-u03b21 may have a promising future for improved cartilage tissue engineering.

Source link: https://europepmc.org/article/MED/36314425


Articular Cartilage Regeneration through Bioassembling Spherical Micro-Cartilage Building Blocks.

Articular cartilage lesions are common in the United States and affect one out of seven adult and many young patients. Cartilage is not able to recover on its own, and is not capable of recovering by itself. Existing medical therapies for articular cartilage lesions have limitations. Cartilage tissue engineering is a promising technique for restoring articular neocartilage. Bioassembly is an emerging technology that builds macro-tissues or micro-precursor tissues as building blocks. We analyzed seven categories of related research, and year trends were displayed for seven categories of related study from a database set of 5069 papers from the last 28 years of literature.

Source link: https://europepmc.org/article/MED/36291114


Collagen-binding peptides for the enhanced imaging, lubrication and regeneration of osteoarthritic articular cartilage.

Human osteoarthritic cartilage and smoother cartilage ex vivo were found in osteoarthritic cartilage and improved lubricated human osteoarthritic tissue ex vivo, as compared to a scrambled sequence. WXPXW was the consensus motif that binds to the u03b11 chain of collagen type XII, according to Molecular docking. Peptides that specifically link to osteoarthritic tissue may help with diagnosis and treatment of osteoarthritic joints.

Source link: https://europepmc.org/article/MED/36229661


Injectable Cell-Laden Polysaccharide Hydrogels: In Vivo Evaluation of Cartilage Regeneration.

To determine the effectiveness of cell-laden hydrogels in tissue formation, 5% w/v hybrid hydrogels, bovine chondrocytes, a mixture of all three, or a combination of both were mixed in a 4:1 ratio and subcutaneously implanted in male and female nude rats' backs to determine cell-laden hydrogels' success in tissue formation. However, in the HA-TA/Dex-TA hydrogels laden with either hMSCs or bCHs, but not with the co-culture, increased tissue invasion and some massive cell infiltration were observed. In addition, we have evidence that host gender plays a role in the success of bCHs encapsulated in HA-TA/Dex-TA hydrogels. Different host responses were found by hydrogels enriched with various types of cells in this research. It can be said that 5% w/v hydrogels with a higher percentage of Dex-TA laden with a higher percentage of bCH-hMSC co-cultures are suitable for injectable applications and in situ cell delivery in cartilage regeneration techniques.

Source link: https://europepmc.org/article/MED/36297870


Uniparental parthenogenetic embryonic stem cell derivatives adaptable for bone and cartilage regeneration.

The uniparental parthenogenetic embryonic stem cells have certain characteristics in common with embryonic stem cells. This research aims to determine the profitability of pESC-based tissue engineering for bone and cartilage reconstruction. In order to produce embryoid bodies, the mouse pESCs were cultured in suspension. paraphrased embryonic mesenchymal stem cells from embryoid bodies were obtained by an adherent culture strategy. We showed that pESCs have the ability to distinguish into all three germ layers. pESC derivatives have a high potential for bone and cartilage regeneration, according to the results of this report.

Source link: https://europepmc.org/article/MED/36228838


Anisotropic hydrogel fabricated by controlled diffusion as a bio-scaffold for the regeneration of cartilage injury† † Electronic supplementary information (ESI) available. See https://doi.org/10.1039/d2ra05141a

Since the next generation of tissue engineering is based on the design of heterogeneous structures that can replicate the body's original biological complexity, controlled manufacturing of anisotropic materials has become a hotspot in materials science, particularly biomaterials. The new fabrication process for producing anisotropic materials for related tissue engineering requires costly and highly specialized equipment, but not every conventional technique can be used to prepare anisotropic materials for corresponding tissue engineering. Anisotropic materials can be quickly applied to a tissue engineering problem: cartilage repair: cartilage damage repair. Controlled diffusion, with the support of buoyancy, has been regarded as a generalized way to prepare materials using a gradient. An anisotropic hydrogel based on a decellularized extracellular matrix is produced and tested herein. The next generation of tissue engineering is based on heterogeneous structures that can replicate the body's original biological complexity. Controlled manufacturing of anisotropic materials has become a hotspot in biomaterials science, leading to a hotspot in biomaterials science.

Source link: https://europepmc.org/article/MED/PMC9535635


Freeze-casting osteochondral scaffolds: The presence of a nutrient-permeable film between the bone and cartilage defect reduces cartilage regeneration.

The trilaminar scaffold demonstrates good biocompatibility and inflammatory control, according to our results, but osteochondral repair is less cost-effective than that of a two-layer scaffold with isolation layer. Thus, the new report reveals that cell infiltration involving stem cells, immune cells, and other bone marrow cells plays a vital role in articular cartilage repair based on a multi-layered scaffold design. STATEMENT OF SIGNIFICANCE: Clinical microfracture therapy can play a certain role in the repair of injured cartilage, but regenerative cartilage is more likely to be fibrocartilage due to bone marrow cell transplantation. We will investigate the effect of each cell type on cartilage repair in our continuing future research due to the multitude of cells in bone marrow.

Source link: https://europepmc.org/article/MED/36210044


PEDF peptide stimulates chondrocyte regeneration in rats with monoiodoacetate-induced articular cartilage injury

Its peptide 29-mer and its associated short peptide 29-mer have been shown to repair tissue damage by inducing various types of stem/progenitor cells localized near the lesions. With MIA-injured AC, we investigated whether the 29-mer is able to cause chondrogenesis in rats. Methods: : By a single intra-articular injection of MIA in the right knee, AC destruction was promoted in Sprague-Dawley rats. BrdU was also injected intraperitoneally into the rats in order to detect cell proliferation in the damaged AC. Dual-immunostaining of BrdU and chondrocyte markers, including aggrecan, collagen type 2 and Sox9, was shown to promote chondrocyte regeneration in the AC. To determine the MIA-induced joint pain, changes in hind paw weight distribution were determined. Conclusions: : The 29-mer/HA product has the ability to stimulate chondrocyte regeneration in the MIA's impaired AC.

Source link: https://europepmc.org/article/PPR/PPR555242

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions