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Cartilage Oligomeric Matrix Protein - Crossref

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Last Updated: 11 January 2023

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Moraxella catarrhalis Evades Host Innate Immunity via Targeting Cartilage Oligomeric Matrix Protein

Abstract Moraxella catarrhalis is a respiratory tract pathogen that causes otitis media in children and acute exacerbations in patients with chronic obstructive pulmonary disease. The majority of clinical isolates of M. catarrhalis are isolated in isolates, but not other tested bacterial pathogens, bond large quantities of COMP. Binding of COMP correlates with M. catarrhalis in human serum by reducing the complement membrane attack complex's bactericidal activity. We also found that COMP reduces bacterial adhesion and uptake by human lung epithelial cells, shielding M. catarrhalis from intracellular destruction by epithelial cells.

Source link: https://doi.org/10.4049/jimmunol.1502071


Human Monoclonal Rheumatoid Synovial B Lymphocyte Hybridoma with a New Disease-Related Specificity for Cartilage Oligomeric Matrix Protein

A heptameric peptide of cartilage oligomeric matrix protein may be identified as the target structure detected by the human synovial B cell hybridoma. In five separate RA patients, the COMP-specific hybridoma's clonyptic VH sequences could also be detected in synovectomy samples from five different RA patients, but not in any of the investigated osteoarthritis patients, showing a preferential use of VH genes closely related to those coded for a COMP-specific Ag receptor in RA synovial B cells.

Source link: https://doi.org/10.4049/jimmunol.166.6.4202


Lubricin binds cartilage proteins, cartilage oligomeric matrix protein, fibronectin and collagen II at the cartilage surface

This investigation was carried out to localize COMP-lubricin complexes within cartilage and determine if other cartilage proteins are involved in anchoring lubricin to the joint. However, mice were still covered with a lubricin layer on their articular cartilage, prompting further investigation into additional lubricin binding mechanisms. Proximity ligation assays on human cartilage biopsies were used to localize additional lubricin binding partners and established that lubricin bound COMP was not limited to collagen alone, but also fibronectin and collagen II on the cartilage surface. Solid phase binding assays with recombinant lubricin fragments were reported and characterized by solid phase binding assays.

Source link: https://doi.org/10.1038/s41598-017-13558-y


Cartilage Oligomeric Matrix Protein-Deficient Mice Have Normal Skeletal Development

ABSTRACT Cartilage oligomeric matrix protein belongs to the thrombospondin family and is primarily present in cartilage. In vivo, we've developed COMP-null mice to investigate COMP's role in vivo. The lack of COMP is not compensated for by any other member of the thrombospondin family's analysis and immunohistochemical evaluation of cartilage, according to both a northern blot and immunohistochemical evaluation of cartilage. The phenotype in PSACH/MED cartilage disorders is not exacerbated by the reduced amount of COMP.

Source link: https://doi.org/10.1128/mcb.22.12.4366-4371.2002


Cartilage oligomeric matrix protein as a marker of progressive liver fibrosis in biliary atresia

Abstract This research sought to determine whether mRNA and protein levels of cartilage oligomeric matrix protein, a glycoprotein that regulates homeostasis of extracellular matrix, were related to clinical indices of biliary atresia patients, and may have been a biomarker for BA severity. COMP numbers in BA patients' circulation were noticeably higher than those in healthy controls. The degree of liver fibrosis was found to be independently related to elevated circulating COMP scores. Elevated circulating COMP levels were significantly correlated with reduced survival of BA patients, according to a Survival study. Both COMP mRNA and protein expressions of BA patients with fibrosis were evidently higher in the liver than those of BA patients without fibrosis and non-BA patients.

Source link: https://doi.org/10.1038/s41598-021-95805-x


Serum cartilage oligomeric matrix protein: is there a repeated bout effect?

The primary aim of the present study was to see if there is a repeated bout effect on cartilage tissue, as shown in the marker serum cartilage oligomeric matrix protein. Two healthy male volunteers underwent two high impact interventions carried out at a 3 week interval. After the second bout, Muscle soreness was determined by a visual analog scale with a maximum pain score of 10. sComp scores did not show a decrease. Unlike the observed repeated bout effect for muscle damage indicators, sCOMP scores do not display a reduced response after two similar loading experiments.

Source link: https://doi.org/10.4081/or.2014.5543


Structure, evolution and expression of zebrafish cartilage oligomeric matrix protein (COMP, TSP5). CRISPR-Cas mutants show a dominant phenotype in myosepta

The gene encoding zebrafish Comp is found on chromosome 11 in synteny with its mammalian orthologs. Zebrafish Comp has a domain name similar to that of tetrapod COMP, and shares 74% sequence similarity with murine COMP. The absence of Comp in zebrafish cartilage indicates that its cartilage function evolved in tetrapods, and not in tetrapods. CRISPR-Cas hit a sequence encoding a calcium binding motif within the first TSP type-3 repeat of zebrafish Comp. Comp zebrafish, a heterozygous and homozygous mutant, had patches of irregular Comp staining in 3 dpf myosepta, indicating a dominant phenotype. The early onset and easy detection of mutant Comp in zebrafish shows that the zebrafish model could be used for large scale screening of small molecules that may increase the functionality or function of disease-associated COMP mutants.

Source link: https://doi.org/10.3389/fendo.2022.1000662


Diagnostic Accuracy of Cartilage Oligomeric Matrix Protein (COMP), for Cartilage Damage in Rheumatoid Arthritis

Background: Cartilage oligomeric matrix protein, a non-collagenous glycoprotein that is mainly localized within the cartilage and can also be present in tendon and synovium. In rheumatoid arthritis, the aim is to correlate serum COMP levels with disease severity and cartilage damage. 88 healthy subjects and 88 RA patients are included in the study population. Result: Serum COMP numbers in RA patients 55. 4 ng/ml were significantly higher than controls 21. 454 ng/ml with a significant p value of= 0. 0001. There was a strong positive correlation between COMP level and disease severity in RA patients with moderate to severe RA patients with moderate or high disease activity scores, which had a significant p value. Serum COMP can be used as a biomarker to determine cartilage loss in RA patients.

Source link: https://doi.org/10.9734/jammr/2020/v32i1630631


Endothelin-1 depletion of cartilage oligomeric matrix protein modulates pulmonary artery superoxide and iron metabolism-associated mitochondrial heme biosynthesis

ET1 also increases superoxide in a way that may possibly hinder mitochondrial heme biosynthesis. In this research, organoid culture of BPA with ET1 raises superoxide in the mitochondrial matrix and extramitochondrial regions associated with COMP depletion, and COMP inhibited these superoxide rises. We investigated ET1, COMP, and COMP siRNA results on FECH, transferrin receptor-1 and soluble guanylate cyclase, as mitochondrial matrix superoxide deposition from prothromo prophyrin IX by lowering Fe 2+ availability and/or ferrochelatase, as well as PpIX and heme measurements. COMPROM reversed these effects of ET1, and COMP produced less PpIX and boosted heme in the absence of ET1. COMP siRNA enhanced PpIX detection and TfR1 expression, as well as reduced the expression of FECH and sGC. Complement depletion by ET1 or siRNA alters pulmonary arterial iron metabolism, resulting in the loss of heme biosynthesis and heme-dependent cGMP mechanisms.

Source link: https://doi.org/10.1152/ajplung.00534.2020

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions