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The roots of Rosemary leaves were macerated with 1 minimum volume of 70% acidic ethanol-water solution for 3 hours, respectively, and their extraction rates were 96 percent and 94 percent, respectively. The supernatant and precipitate were separately isolated from a water-soluble extract containing 6. 84 percent rosmarinic acid and a lipid-soluble extract containing 31. 18 percent carnosic acid after the deposition of ethanol. The prepared carnosic acid can inhibit the proliferation of Gram-positive bacteria and Edwardsiella tarda growth. This analysis made full use of rosemary plants, greatly lowering rosemary extract extraction and carnosic acid extraction costs, which greatly reduced rosemary extract preparation and carnosic acid extraction costs. The antibacterial activity of carnosic acid can be used in industry and aquaculture as an antimicrobial agent.
Source link: https://europepmc.org/article/MED/IND607861943
Rosemary extracts are widely used as natural preservatives due to their antimicrobial and antioxidant activities, which are attributed to the phenolic diterpenoid carnosic acid. Growers are recognized based on CA content in their rosemary leaf harvest, which is based on CA content. Leaf CA measurements are inaccurate and often time-consuming, and often time-consuming. This preliminary report provides a non-destructive measure for in vivo estimation of CA content in various rosemary cultivars based on their canopy's reflectance spectra. Using results obtained from 79 rosemary cultivars, correlations were established between measured CA content by high-performance liquid chromatography and leaf reflectance spectra, standardized spectral indices, and latent components obtained by genetic algorithm-based partial least squares regression. The residual prediction deviations were reported as 2. 71 and 2. 05, respectively, with 0. 9 percent and 1. 15% CA content obtained for calibration and cross-validation.
Source link: https://europepmc.org/article/MED/IND607818809
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