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Cargo - Europe PMC

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Last Updated: 14 September 2022

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Systematic analysis of microtubule plus-end networks defines EB-cargo complexes critical for mitosis in budding yeast

Open questions regarding which EB binding partners are most important for cell division and how cells can build a microtubule cytoskeleton without the presence of an EB protein are among cell division's most important for cell division. Here we show that the budding yeast EB protein Bim1 performs its key mitotic roles as part of two cargo complexes-Bim1-Kar9 in the cytoplasm and Bim1-Kar3 in the nucleus. Our report also highlights compensatory mechanisms that allow cells to proliferate in the absence of Bim1 and Kargo complexes.

Source link: https://europepmc.org/article/PPR/PPR541977


Role of actin cytoskeleton in cargo delivery mediated by vertically aligned silicon nanotubes.

The actin cytoskeleton plays a vital role in mRNA delivery by silicon nanotubes in this research by reducing actin dynamics by two drugs-cytochalasin D and jasplakinolide. In comparison to untreated controls, actin inhibition 12 h before SiNT-cellular interfacing significantly dampens mRNA delivery into mouse fibroblast GPE86 cells. However, actin inhibition, which began 2 h after the establishment of the GPE86 cell-SiNT interface, has negligible effect on mRNA transfection, with only > 80% effectiveness for both Cyto D and Jas treatment groups.

Source link: https://europepmc.org/article/MED/36076230


Mapping autophagosome contents identifies interleukin-7 receptor-α as a key cargo modulating CD4+ T cell proliferation.

T cells from CD4+ T cells, which are important cells involved in the orchestration of humoral and cytotoxic immune responses, are key to the orchestration of humoral and cytotoxic immune responses. It is known that CD4+ T cell proliferation is linked to autophagy, but information regarding the autophagosome cargo involved is missing. Interleukin-7 receptor-u03b1, a cytokine receptor commonly present in na's and memory T cells, is reproducibly present in autophagosomes of activated CD4+ T cells. Externalization shows an increase in interleukin-7 receptor-u03b1 surface expression, although no change was found in internalisation.

Source link: https://europepmc.org/article/MED/36055998


A dueling-competent signal-sensing module guides precise delivery of cargo proteins into target cells by engineered Pseudomonas aeruginosa

We screened H1-T6SS coated substrates and created Tse6 N as the most useful credition tag for Cre delivery using a Cre-recombinase-dependent reporter. In a mixed population, T6SS-Cre cargo can be transported in a discriminately deliver the Tse6 N -Cre cargo into the cytosol of T6SS + but not T6SS u2212 Vibrio cholerae cells.

Source link: https://europepmc.org/article/PPR/PPR539246


Visualization of intercellular cargo transfer using upconverting nanoparticles.

The intercellular transport in live HeLa cells was clearly seen with real-time, long-livesicle tracking with real-time, long-term single-vesicle tracking. In addition, it was found that the endocytosed UCNPs can be transmitted by the encapsulation of EVs labeled with fluorescent proteins.

Source link: https://europepmc.org/article/MED/36053238


MFA-net: Object detection for complex X-ray cargo and baggage security imagery.

The object-scale variant of the detector backbone converts the detector backbone's common convolution into a conditional convolution by aggregating the characteristics from multiple dilated convolutions using dynamic feature selection to address the object-scale variant problem. The proposed attention and fusion modules in second, as well as the object recognition and occlusion problem, are combined in a two-fold pyramid network. Without requiring further ground-truth data, the auxiliary point detection head deploys an assistant to forecast the new key points of the bounding box on the third page. A MFA-net prototype in the baggage domain and our data in the cargo domain were tested on two large-scale X-ray image datasets from various sources: a Security Inspection X-ray dataset in the baggage domain and our database named CargoX. Thus, adopting the latest object detectors on X-ray security images will enhance the detection capability of the current object detectors.

Source link: https://europepmc.org/article/MED/36048779


Orthogonal Covalent Entrapment of Cargo into Biodegradable Polymeric Micelles via Native Chemical Ligation.

Native chemical ligation is a mild bio-orthogonal reaction involving a N- terminal cysteine residue and a thioester that continues under physiological conditions. We created a trifunctional cross-linker here in the micelle core cross-linking reaction and an azide residue for ring-strained fluorescent dye conjugation. We used this strategy to thermosensitive methacrylamide glycol-based block copolymers of a core-cross-linked polymeric micelle system by attaching thioester residues for NCL cross-linking with the trifunctional cross-linker under physiological conditions. The dye entrapped CCPMs were found to be effective internalization of the dye entrapped CCPMs, which did not affect cell viability up to a polymer concentration of 2 mg/mL in PC3 cells, demonstrating safe cell viability. These fluorescent dye entrapped CCPMs can be used in diagnostic imaging, and the chemistry uncovered in this research provides a stepping stone toward covalently entrapped fragile drug compounds with tunable release in CCPMs.

Source link: https://europepmc.org/article/MED/36044412


Impact of mesenchymal stromal cell-derived vesicular cargo on B-cell acute lymphoblastic leukemia progression.

For example, small extracellular vesicles are released by Leukemia cells and interact with their surrounding bone marrow microenvironment, making it hospitable to leukemia cell survival. B-ALL cells with inadequate MSC and their sEV lack increased amounts of syntenin and syndecan-1, leading to a more emmature B cell phenotype and increased number/function of leukemia-initiating cells by focal adhesion kinase and AKT signaling in B-ALL cells. BCR-ABL1+ B-ALL and Ex vivo pretreatment of LIC with sEV obtained from PLEKHM1-deficient MSC accelerated murine and human BCR-ABL1+ B-ALL. B-ALL patients with B-ALL had a reduced risk of PLEKHM1+ MSC, according to a consistent percentage. In summary, our results show that BMM-derived sEV for driving specifically BCR-ABL1+ B-ALL, potentially contributing to its poor prognosis in comparison to BCR-ABL1-B-ALL, and that the unveiled secretion of inflammatory cytokines by cancer cells in general can also modulate tumor microenvironment.

Source link: https://europepmc.org/article/MED/36044386


Cargo transport by catchbonded motors in optical trapping assays

Experiments using optical trapping assays, which were performed with optical trapping assays, have been an important way of accurately measuring molecular forces generated by molecular motor proteins. In a harmonic sense, we investigate the transport characteristics of cargo transported by dynein molecular motors both singly and in teams, which mimics the dynamic force experienced by cargo in an optical trap. Even for cargo transported by a single motor, catchbonding may play a part, according to our findings, especially for cargo transported by a single motor, depending on the force scale that marks the onset of the catch bond.

Source link: https://europepmc.org/article/PPR/PPR538245

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions