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In clinical trials, cardiosphere-derived cells isolated from human cardiac biopsies have been shown to have disease-modifying bioactivity. We investigated the molecular identity of CDCs using single-cell RNA sequencing in comparison to cardiac non-myocyte and non-hematopoietic cells. We identified CDCs as a distinct and mitochondria-rich cell type with biological similarities with non-myocyte cells but not with cardiac progenitor cells obtained from human-induced pluripotent stem cells. CXCL6 first emerged as a new CDC-specific indicator. We found transcriptomic similarities between CDCs and CFs by analyzing sc-RNAseq results from human right atrial biopsies in comparison with CDCs. Infant and adult CDC sc-RNAseq results found GO-terms associated with cardiac growth by direct comparison of infant and adult CDC scRNAseq results. CDC EVs were boosted in vitro angiogenesis and did not promote scarring, and did not encourage scarring. CDCs obtain certain bioactivities, including angiogenic ability, that may have the ability to alter disease in certain disorders.
Source link: https://europepmc.org/article/MED/35258704
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