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Cardiomyocytes Stem Cells - Europe PMC

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Last Updated: 23 August 2022

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Regenerative Potential of Injectable Platelet-Rich Fibrin to Accelerate Differentiation of Adipose-derived Mesenchymal Stem Cells into Cardiomyocytes-like Cells

Infarct, cell therapy is considered as the most effective therapy for cardiac repair. Objective: To accelerate differentiation of adipose-derived mesenchymal stem cells into cardiomyocyte-like cells, we need to determine the benefit of injectable platelet-rich fibrin. Methods: This review is the first in an experimental randomized post-test design study. Mesenchymal stem cells were isolated from adipose tissues and cultured until 4 passages, and Adipose-derived mesenchymal stem cells were isolated from adipose tissue cultured until 4 passages. Using flowcytometry, the characteristics of adipose-derived mesenchymal stem cells were determined. The differentiation to cardiomyocyte was determined by using flowcytometry on the fifth day and troponin was performed using immunocytochemistry on the tenth day. Flowcytometry on GATA-4 expression demonstrated significant differences in comparison to negative and positive controls on the addition of platelet-rich fibrin. Conclusion: Injectable platelet-rich fibrin has a beneficial role in the transformation of adipose-derived mesenchymal stem cells into cardiomyocyte-like cells.

Source link: https://europepmc.org/article/PPR/PPR527159


Three-Dimensional Poly-(ε-Caprolactone) Nanofibrous Scaffolds Promote the Maturation of Human Pluripotent Stem Cells-Induced Cardiomyocytes.

Although stem cell-derived cardiomyocytes have been shown to be a new avenue for heart regeneration, the clinic application is still suffering from a lack of maturity. According to new studies, stem cell function is closely associated with the extracellular matrix's nanoscale geometry/topography. However, the effects of 3D nanofibrous scaffolds in iPSC-CM maturation remain uncertain. The mature morphology of 3D-shaped iPSC-CMs leaded to increased calcium transient kinetics, with increased calcium peak transient amplitude and maximum upstroke speed.

Source link: https://europepmc.org/article/MED/35979378


A Preclinical Study on Brugada Syndrome with a CACNB2 Variant Using Human Cardiomyocytes from Induced Pluripotent Stem Cells.

Using this method, the aim of this study was to develop a cellular model of BrS in the presence of a CACNB2 variant of uncertain importance using human-induced pluripotent stem cell-derived cardiomyocytes and assess drug effects using this model. Methods and findings: This research used cells from a patient with Brugada syndrome and recurrent ventricular fibrillation with a missense version of CACNB2 that was missing in CACNB2 and other healthy individuals as well as three healthy individuals. Compared to the healthy control hiPSC-CMs, the BrS patient's hiPSC-CMs had a significantly reduced L-type calcium channel current. Compared to healthy hiPSC-CMs, the protein expression of CACNB2 of the BrS-patient's hiPSC-CMs was significantly reduced. These results show that the CACNB2 gene variant in hiPSC-CMs from the BrS patient led to the loss of-of-function of L-type calcium channels in hiPSC-CMs.

Source link: https://europepmc.org/article/MED/35955449

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions