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Cardiomyocytes Stem Cells - Crossref

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Last Updated: 23 August 2022

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Characteristics of calcium sparks in cardiomyocytes derived from embryonic stem cells

spontaneous Ca 2+ sparks releasing Ca 2+ in embryonic stem cell-derived cardiomyocytes via ryanodine receptor channels were characterized and correlated to the expression of RyRs and the Ca 2+ load of the sarcoplasmic reticulum. Global intracellular Ca 2+ concentration fluctuations occurred in very early developmental stage cardiac precursor cells, during which Ca 2+ sparks and contractions were non-existent, although Ca 2+ sparks and contractions were absent. The characteristics of Ca 2+ sparks occurring in cardiomyocytes that differ from ES cells can be determined by the SR's Ca 2+ load and/or the number of RyRs.

Source link: https://doi.org/10.1152/ajpheart.2001.281.1.h411


Regenerative Potential of Injectable Platelet-Rich Fibrin to Accelerate Differentiation of Adipose-derived Mesenchymal Stem Cells into Cardiomyocytes-like Cells

Cell therapy is considered an effective treatment for cardiac repair in the infarct zone. Objective: To promote the differentiation of adipose-derived mesenchymal stem cells into cardiomyocyte-like cells, it is necessary to determine the value of injectable platelet-rich fibrin. This is a real scientific randomized post-test design study, according to the methods used in this paper. Mesenchymal stem cells were isolated from adipose tissues and cultured until four passages, according to Adipose-derived mesenchymal stem cells isolated from adipose tissues and cultured until 4 passages. The characteristics of adipose-derived mesenchymal stem cells were determined by the expression of CD34-, CD45-, and CD 105+ using flowcytometry. The differentiation to cardiomyocyte was determined by the differentiation to cardiomyocyte on the tenth day and troponin was performed using flowcytometry on the fifth day and troponin was carried out using immunocytochemistry on the tenth day. Flowcytometry on GATA-4 expression revealed significant difference on the addition of platelet-rich fibrin as compared to negative and positive controls. Conclusion: Injectable platelet-rich fibrin has a promoting role in the transformation of adipose-derived mesenchymal stem cells into cardiomyocyte-like cells.

Source link: https://doi.org/10.20944/preprints202208.0070.v1


Brugada Syndrome: Different Experimental Models and the Role of Human Cardiomyocytes From Induced Pluripotent Stem Cells

Brugada syndrome, an inherited and rare cardiac arrhythmogenic disorder, is associated with an elevated risk of ventricular fibrillation and sudden cardiac death. Only a few BrS studies have used human-u2010induced pluripotent stem cell-u2013derived cardiomyocytes, the bulk of which have been focused on genotypeu2013phenotype correlations and drug screening. This review sought to compare BrS models to get a better understanding of the roles of human-u201induced pluripotent stem cell stem cells in current BrS research and personalized medicine at a later stage.

Source link: https://doi.org/10.1161/jaha.121.024410


Three-Dimensional Poly-(ε-Caprolactone) Nanofibrous Scaffolds Promote the Maturation of Human Pluripotent Stem Cells-Induced Cardiomyocytes

Although pluripotent stem cell-derived cardiomyocytes have been used as a new source of heart transplantation, the clinic application is severely hindered by the lack of maturity. However, the effects of 3D nanofibrous scaffolds in maturation of iPSC-CMs remain unclear. We investigated the effects of restructuring iPSC-CMs in 3D nano-scaffolds on cell morphology, cardiac-specific structural protein, gap junction, and calcium transient kinetics. The mature morphology of 3D-shaped iPSC-CMs contributed to enhanced calcium transient kinetics, with increased calcium peak transient amplitude and maximum upstroke speed.

Source link: https://doi.org/10.3389/fcell.2022.875278


A Preclinical Study on Brugada Syndrome with a CACNB2 Variant Using Human Cardiomyocytes from Induced Pluripotent Stem Cells

Aims: Brugada syndrome has been linked to several gene variants in the sodium channels, as well as calcium channels. Objectives: The aim of this research was to establish a cellular model of BrS in the presence of a CACNB2 variant of uncertain value, using human-induced pluripotent stem cell-derived cardiomyocytes and testing drug effects using this method. Methods and findings: This review collected cells from a patient with Brugada syndrome and recurrent ventricular fibrillation with a missense version of CACNB2 that was missing in CACNB2, as well as three healthy individuals. For this research, these cells, as well as CRISPR/Cas9 corrected cells, were obtained from skin biopsies of healthy people and the BrS patient. The BrS patient's hiPSC-CMs had a significantly reduced L-type calcium channel current relative to the healthy control hiPSC-CMs. Compared to healthy hiPSC-CMs, the protein expression of CACNB2 of the BrS-patient's hiPSC-CMs was significantly reduced.

Source link: https://doi.org/10.3390/ijms23158313


Melphalan induces cardiotoxicity through oxidative stress in cardiomyocytes derived from human induced pluripotent stem cells

Treatment-induced cardiotoxicity is a common noncancer-related cause of acute and late onset morbidity and mortality in cancer patients on antineoplastic drugs such as melphalan-u2014increasing clinical case reports, according to researchers, it can cause cardiotoxicity, severe arrhythmias, heart failure, and heart failure. We investigated cardiomyocytes derived from human-induced pluripotent stem cells to investigate the cellular and molecular mechanisms of melphalan-induced cardiotoxicity and the development of potential target therapeutics. According to the proteomic and transcriptomic reports, multiple other signaling pathways, including the p53 and transforming growth factor-u03b2 signaling pathways were also implicated in melphalan-induced cardiotoxicity. Melphalan causes cardiotoxicity by the oxidative stress pathway. This study provides a unique resource of the global transcriptomic and proteomic data for melphalan-induced cardiotoxicity and may help to prevent and treat melphalan-induced cardiotoxicity.

Source link: https://doi.org/10.21203/rs.3.rs-45895/v2


Melphalan induces cardiotoxicity through oxidative stress in cardiomyocytes derived from human induced pluripotent stem cells

Abstract Background: Treatment-induced cardiotoxicity is a common noncancer-related cause of acute and late onset morbidity and mortality in cancer patients on antineoplastic drugs, including melphalan, and heart failure in cancer patients on antineoplastic drugs such as melphalan. Here we explored the cellular and molecular mechanisms of melphalan-induced cardiotoxicity, as well as potential target therapeutics. According to the proteomic and transcriptomic studies, multiple other signaling pathways, including the p53 and transforming growth factor-u03b2 signaling pathways, were also implicated in melphalan-induced cardiotoxicity. This review provides a unique resource of the global transcriptomic and proteomic datasets for melphalan-induced cardiotoxicity, and may help to identify and treat melphalan-induced cardiotoxicity.

Source link: https://doi.org/10.21203/rs.3.rs-45895/v1


A three-dimensional culture system for generating cardiac spheroids composed of cardiomyocytes, endothelial cells, smooth-muscle cells, and cardiac fibroblasts derived from human induced-pluripotent stem cells

However, recently differentiated hiPSC-CMs tend to more closely resemble adult CMs than mature CMs, and new strategies for increasing CM maturation can be both intricate and labor-intensive. According to this, the manufacture of CMs for industrial and industrial purposes would require new elementary techniques for boosting CM maturity. Instead of as two-dimensional monolayers, CMs tend to develop a more mature phenotype in a three-dimensional environment, and ECs, SMCs, and CFs play a role in both CM maturation and electrical function.

Source link: https://doi.org/10.3389/fbioe.2022.908848


Generation of cardiomyocytes from human induced pluripotent stem cells resembling atrial cells with ability to respond to adrenoceptor agonists

ABSTRACT Cardiovascular disease is the most common cause of global mortality and morbidity. Human-induced pluripotent stem cell-derived atrial cardiomyocytes represent an exciting new model for AF, but human-induced pluripotent stem cell-derived atrial cardiomyocytes are an excellent new model for AF, but humanized stem cell-derived atrial cardiomyocytes are still insufficient in translational ability, and thus are currently ineffective. We present a new strategy for growing cardiomyocytes resembling atrial cells with a combination of Gremlin 2 and retinoic acid therapy of human iPSCs. More than 80% of myocytes generated by this approach had rod-shaped morphology, expression of cardiomyocyte proteins, and generally a striated appearance, all of which were remarkably similar to adult atrial myocytes. Myocytes were chemically reticent until stimulated to fire action potentials with an atrial myocyte profile and an amplitude of about 100 mV, resulting from a resting potential of about u221270 mV. Our latest technology, on the other hand, provides an effective way for determining human atrial myocytes with mature characteristics of hiPSCs.

Source link: https://doi.org/10.1101/2022.07.20.499551

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions