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Cardiomyocytes - Europe PMC

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Last Updated: 10 September 2022

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Contractile Force of Transplanted Cardiomyocytes Actively Supports Heart Function After Injury.

Background Transplantation of pluripotent stem cell-derived cardiomyocytes is a promising therapeutic approach for cardiac recovery, and the first clinical trials in patients with heart disease have began. In a guinea pig cardiac injury model, we injected cardiomyocytes with an optogenetic off-on switch. Implications Light-inhibited cardiomyocyte contractility deficionation in a rapid decrease in left ventricular function in engrafted cardiomyocyte contractility in u224850% animals that was completely reversible with the advent of photostimulation.

Source link: https://europepmc.org/article/MED/36073365


Comparing the signaling and transcriptome profiling landscapes of human iPSC-derived and primary rat neonatal cardiomyocytes

However, the inclusion of healthy human cardiac tissue samples for basic science research was beyond reach. Post-mortem tissue or biopsies from diseased patients, which are still scarcely available, made it possible to investigate end-stage heart disease, but not so healthy human heart tissues for basic science research was out of reach. In vivo or in vitro cultures, Rodent models became increasingly popular surrogates to model the human heart. The use of human inducible stem cell derived cardiomyocytes was a renewed possibility more recently, due to questions over animal to human translation, as well as cross-species variations. Our investigations of nuclear PKA and ERK1/2 signaling revealed distinct response clusters in rat and human CMs using automated high content microscopy. Overall, our report shows that human stem cell-derived cardiomyocyte models of the cardiomyocyte have significant benefits and should be taken advantage of.

Source link: https://europepmc.org/article/PPR/PPR540853


Ultra-low power deep sustained optogenetic excitation of human ventricular cardiomyocytes with red-shifted opsins: A computational study.

Including newly identified opsins, this article outlines the development of accurate theoretical models of optogenetic surveillance of HVCMs. Action potentials in each opsin-expressing HVCMs can only be evoked in a limited range of irradiances under continuous illumination. Ongoing APs in ChRmine-expressing HVCMs can be reduced by continuous illumination of 585 nm light at 2 u03bcW/mm 2. Up to u223c mm and 10. 2 mm with ChRmine on illuminating the outer surface of pericardium at stable irradiance at 585 nm and 650 nm, respectively. Abstract The key problem in cardiac optogenetics is the ability to have low-power, high-fidelity, and deep excitation of cells with minimal invasiveness and heating. At 585 nm, action potentials in ChRmine expressing HVCMs can be triggered at 6 bcW/mm2 and 0. 7 bcW/mm2, two orders of magnitude less than ChR2, with ChRmine expressing HVCMs at 6 u03bcW/mm2 and 0. 7 mm2 aho3 nm, both orders of magnitude lower than ChR2, which are two orders of magnitude lower than ChRmine u03bcW/mm2 and nm2 and 1. 2 u03bc expressing ChRmine expressing HVCMs triggering HVCMs a u03bcW/mm2 and a u03bcW/mm2 and u03bcW/mm2 and bcW/mm2 and bcW/mm2 nm2 and 0 mm2 and e mm2 and u03bcW/mm2 and em2 nm2 and nm2 a & Minimum charge transfer during AP has been established, providing vital tissue protection under sustained excitation. It has been determined that an optimal irradiance range for each opsin to excite HVCMs. Each AP in a sequence remains most predictable and an order lower respectively in ChRmine-expressing HVCMs, under optimum photostimulation conditions, each opsin can precisely excite APs up to 2. 5 Hz, while the latency and power of light pulse for each AP remain most stable and an order lower respectively. Figure legend from the abstract sketch of the pericardium. By placing the light source near the outer surface of the pericardium, deep optogenetic excitation of opsin-expressing cardiomyocytes can be triggered. With ChRmine, excitation of cardiomyocytes up to 10. 2 mm and 7. 46 mm is possible.

Source link: https://europepmc.org/article/MED/36068951


ACTN2 Mutant Causes Proteopathy in Human iPSC-Derived Cardiomyocytes.

Compared to ACTN2wt in 2D-cultured hiPSC-CMs, ACTN2mut showed a higher incidence of multinucleation, protein aggregation, hypertrophy, myofibrillar disarray, and initiation of both the ubiquitin-proteasome system and the autophagy-lysosomal pathway. In addition, the expression of ACTN2mut was shown to a significant decrease in sarcomere-associated protein levels in 2D-cultured hiPSC-CMs and force loss in engineered heart tissues. Our report also shows that proteolytic pathways in ACTN2mut hiPSC-CMs that can be related to human ACTN2 aggregation are likely to cope with ACTN2 aggregation and, as a result of this cellular pathology caused by this ACTN2 variant's human ACTN2-associated cardiomyopathies, may contribute to human ACTN2 mutation cardiomyopathies.

Source link: https://europepmc.org/article/MED/36078153


Investigating and Resolving Cardiotoxicity Induced by COVID-19 Treatments using Human Pluripotent Stem Cell-Derived Cardiomyocytes and Engineered Heart Tissues.

Coronavirus disease 2019 is the most common disease worldwide. Human pluripotent stem cell-derived cardiomyocytes are a valuable tool for determining drug-induced cardiotoxicity. Human engineered heart tissue model is used to analyze the cardiotoxic effects of these medications' cardiotoxic effects, but it is found that they weaken hEHT contractile function. It has been shown that ceftiofur hydrochloride, astaxanthin, and quetiapine fumarate can reduce the cardiotoxicity of remdesivir, with astaxanthin being the most popular one.

Source link: https://europepmc.org/article/MED/36055796


MEA-integrated cantilever platform for comparison of real-time change in electrophysiology and contractility of cardiomyocytes to drugs.

Cardiotoxicity is mostly assessed in animal models using conventional drug screening methods. Despite significant advancements in drug screening techniques, the challenge in measuring electrophysiology and contractile profile together, and numerous other factors, cardiovascular safety testing in potential drugs can be affected by cardiovascular risk assessment. Cardiomyocytes can be detected herein by a microelectrode array integrated SU-8 cantilever for dual and simultaneous measurement of electrophysiology and contractility of cardiomyocytes. Using conventional photolithographic methods, the SU-8 cantilever is integrated with a microelectrode array. Drug experiments were done to determine the reliability of the C-MEA platform that uses three cardiovascular drugs. Multiple contractility-based measurements are used to compare the effects of ion channel blockers on the field potential duration of the cardiomyocytes. The contraction-relaxation duration profile is fairly close to that of FPD in standardized drugs, with a very close similarity. Blebbistatin, a well-known myosin II inhibitor, has mainly affected the cardiovascular profile of cardiomyocytes but not their field potential, with no apparent correlation between contractility and field potential profiles.

Source link: https://europepmc.org/article/MED/36070668


Adrenergic Receptor Regulation of Mitochondrial Function in Cardiomyocytes.

Abstract Adrenergic receptors are G protein-coupled receptors that are stimulated by catecholamines in a wide variety of physiological functions in tissue types. Both u03b11- and u03b2-ARs have been uncovered in cardiomyocytes and regulate cardiac contraction and hypertrophy in different molecular pathways. As an adaptive stress response, acute activation of cardiomyocyte u03b2-ARs increases heart rate and contractility. However, chronic u03b2-AR stimulation contributes to heart failure's pathobiology. By contrast, growing evidence shows that u03b11-ARs do serve defensive roles, which may help to minimize the deleterious effects of continuous u03b2-AR activation.

Source link: https://europepmc.org/article/MED/35170492


Bioenergetic and Metabolic Impairments in Induced Pluripotent Stem Cell-Derived Cardiomyocytes Generated from Duchenne Muscular Dystrophy Patients.

Mutations in the dystrophin gene and dilated cardiomyopathy, which is a significant cause of morbidity and mortality in DMD patients, is DMD patients. Adult DMD CMs had reduced energy metabolism and abnormal mitochondrial structure and function, according to We found that. Mitol activities in DMD adult CMs were attenuated by 45-48%, whereas the 7y CM's were similar to that of healthy CMs. In DMD CMs but not in 7y CMs, there was a 75% decrease in the mitochondrial ATP production rate relative to healthy iPSC-CMs. DMD iPSC-CMs have bioenergetic and metabolic deficiencies that are related to rhythm disturbances corresponding to the patient's phenotype, thereby establishing new target goals for relieving cardiomyopathy in DMD patients.

Source link: https://europepmc.org/article/MED/36077200


α -Cyperone Protects Cardiomyocytes against Oxygen-Glucose Deprivation-Induced Inflammation and Oxidative Stress by Akt/FOXO3a/NF- κ B Pathway.

Aims of this research The aim of this research is to determine the underlying mechanism of a u03b1-cyperone reaction in oxygen and glucose deprivation-induced myocardial injury. Cell proliferation and apoptosis were found by cell proliferation and apoptosis, respectively, using the cell counting kit-8 assay and flow cytometry. The protective effect of u03b1-cyperone against OGD-induced myocardial injury was reduced by MK-2206 therapy. Following Bay11-7082 therapy, u03b1-cyperone was not added to cardiomyocytes, and there was no apparent improvement in the viability and apoptosis. Cyperone shielded cardiomyocytes from OGD-induced inflammation and oxidative stress by the Akt/FOXO3a/NF-ba B axis, resulting in the uk3b1 B axis.

Source link: https://europepmc.org/article/MED/36072899

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions