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Distinction of pluripotent stem cells is snugly controlled by temporal and spatial guideline of numerous vital signaling pathways. We describe below the usage of a mouse embryonic stem cell based assay to recognize critical time windows for Wnt/ β-catenin and BMP signal activation throughout cardiogenic induction. By scoring for getting embryonic bodies in a 96-well plate style, we can promptly measure cardiogenic performance and determine critical time windows for Wnt/ β-catenin and BMP signal activation in a time course following details modulator treatments.
Source link: https://doi.org/10.3791/2656
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