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Cardiogenic - U.S. Department of Veterans Affairs

Summarized by Plex Scholar
Last Updated: 10 November 2021

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Modified mouse embryonic stem cell based assay for quantifying cardiogenic induction efficiency.

Distinction of pluripotent stem cells is snugly controlled by temporal and spatial guideline of numerous vital signaling pathways. We describe below the usage of a mouse embryonic stem cell based assay to recognize critical time windows for Wnt/ β-catenin and BMP signal activation throughout cardiogenic induction. By scoring for getting embryonic bodies in a 96-well plate style, we can promptly measure cardiogenic performance and determine critical time windows for Wnt/ β-catenin and BMP signal activation in a time course following details modulator treatments.

Source link: https://doi.org/10.3791/2656

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions