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Carcinoma invasion signaling - Europe PMC

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Last Updated: 03 May 2022

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EGF/EGFR Promotes Salivary Adenoid Cystic Carcinoma Cell Malignant Neural Invasion via Activation of PI3K/AKT and MEK/ERK Signaling.

Background Salivary cystic carcinoma is one of the most common malignant tumor tumors of the salivary gland, and 32. 4-72 percent of SACC cases exhibit neural invasion, but the exact mechanism underlying the high invasion potential of SACC remains unclear. Methods The present research investigated the role of epidermal growth factor receptor in AKT inhibition- or mitogen-activated protein kinase-mediated NI and epithelial-mesenchymal transition in SACC cells using EGFR, PI3K, and MEK inhibitors. Cell viability of the SACC 83 cell viability was determined by an MTT assay, and a wound healing assay was used to measure cell migration. In SACC, the present findings show the importance of suppressed EGFR/AKT/MEK signaling by neural-tumor co-culture in vitro. In addition, our preclinical study shows that injection of EGFR, PI3K, and MEK inhibitors inhibited tumor formation and NI of SACC cells in nude mice.

Source link: https://europepmc.org/article/MED/35410600


Regulation of growth, invasion and metabolism of breast ductal carcinoma through CCL2/CCR2 signaling interactions with MET receptor tyrosine kinases.

With over 60,000 patients diagnosed annually in the United States annually, ductal carcinoma in situ is the most common form of early-stage breast cancer. Up to 20% patients experience disease recurrence, indicating that standard treatments do not adequately treat DCIS for a subset of patients. Recent studies show that elevated CCL2/CCR2 expression in breast epithelial cells promotes IDC formation. According to Phospho-protein array profiling, CCL2 promoted the phosphorylation of MET receptor kinases in breast cancer cells. CCL2/CCR2 expression and activity in breast cancer cells, according to extracellular flux analysis and biochemical assays, stimulated glycolytic enzyme expression and activity. Through MET-dependent pathways, CRISPR cell proliferation, survival, migration, and glycolysis can be facilitated by MET-dependent pathways, leading to MET's rejection and pharmacologic inhibition of MET. Met receptor function, according to a summary, is an important factor in early-stage breast cancer progression and metabolism, which has important clinical implications.

Source link: https://europepmc.org/article/MED/35405500


Long non-coding RNA NRAV enhances proliferation and invasion of hepatocellular carcinoma cells by modulating the Wnt/β-catenin signaling pathway.

However, the role of long-coding RNA NRAV in HCC has yet to be clarified. NRAV plays upmodulating role in HCC cells and tissues, and patients with high NRAV levels showed a poor prognosis, according to bioinformatics prediction and real-time quantitative polymerase chain reaction validation. The cell proliferation capacity of the cells was determined assay by cell colony formation assay, and transwell invasion experiments were conducted to determine the cell invasion ability. We performed a dual luciferase assay to determine the correlation between NRAV and miR-199a-3p and CDGSH iron-sulfur domain-containing protein 2 in order to determine the connection between NRAV and miR-199a-3p and CDGSH iron-sulfur domain-containing protein 2. The overexpression of NRAV increased the HCC cell viability, proliferation, and invasion, although invasion was severely hampered by decreased expression of NRAV. miR-199a-3p was not only a target for NRAV but also interacts with the 3' UTR of CISD2 in HCC cells, according to the dual-luciferase assay.

Source link: https://europepmc.org/article/MED/35436415


Vacuolar protein sorting-associated protein 72 homolog (VPS72) binding to lysine acetyltransferase 5 (KAT5) promotes the proliferation, invasion and migration of hepatocellular carcinoma through regulating phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway.

This paper sought to clarify the role and mechanism of vacuolar protein sorting-associated protein 72 homolog 72 homolog in the development of hepatocellular carcinoma. VPS72 expression in hepatocellular carcinoma tissues and the prognostic correlation were first determined by the GEPIA2 database. VPS72 expression was found in several hepatocellular carcinoma cell lines using Western blotting and RT-qPCR assays. Cell proliferation in HuH-7 cells with VPS72 silencing was investigated by cell counting kit-8 and colony formation. According to Co-immunoprecipitation assay, the association between VPS72 and lysine acetyltransferase 5 was forecast by bioGRID, STRING, and GEIPA2 databases. KAT5 was overexpressed to see if VPS72 could slow the progression of hepatocellular carcinoma by binding to KAT5. The regulation of the PI3K/AKT signaling pathway promotes hepatocellular carcinoma progression.

Source link: https://europepmc.org/article/MED/35383533


Overexpression of microRNA-107 suppressed proliferation, migration, invasion, and the PI3K/Akt signaling pathway and induced apoptosis by targeting Nin one binding (NOB1) protein in a hypopharyngeal squamous cell carcinoma cell line (FaDu).

Hypopharyngeal squamous cell carcinoma is one of the most common head and neck cancers, with a poor prognosis due to its aggressivity. We discovered that miR-107 expression was significantly reduced in HSCC tissues in comparison to the paracancer tissues. In addition, dual-luciferase reporter Assay reported that miR-107 could bind to the 3'UTR of Nin one binding protein 1, and that elevated NOB1 levels in HSCC tissues and a negative correlation between miR-107 and NOB1 were discovered. MiR-107 played a key role in FaDu cell behavior by modulating NOB1 by modifying NOB1. MiR-107 could prevent FaDu cell proliferation, migration, invasion, and exapoptosis by targeting NOB1 through the PI3K/Akt pathway, according to the above findings, suggesting that miR-107/NOB1 axis may play a key role in FaDu HSCC development.

Source link: https://europepmc.org/article/MED/35294329


CircRNA_0008194 functions as a ceRNA to promote invasion of hepatocellular carcinoma via inhibiting miR-190a/AHNAK signaling pathway.

Background Hepatitis B virus infection was identified as the leading risk factor of hepatocellular carcinoma in China, causing a high morbidity and mortality. When overexpressing via lentivirus infection, the loss of function and gain of function have been achieved by knocking-down circRNA using the shRNA system. Dual-luciferase reporter assay was used to determine circRNA binding to miRNA and target genes. To determine the binding ability of non-coding RNAs, we used an immunoprecipitation technique. The positive correlation between circum_0008194 and AHNAK was also reported. In vitro, we found that circ_0008194 could cause cell migration. Circulo_0008194 developed the binding ability with miR-190a, which led to the suppression of miR-190a expression, causing AHNAK's competitive inhibition, resulting in the promotion of EMT. Conclusion Our findings showed that circ_0008194 could act as a sponge to absorb miR-190a, thus promoting the expression of AHNAK and encouraging liver cancer metastasis.

Source link: https://europepmc.org/article/MED/35199873

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions