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Despite the fact that the aerial part extract of Alchemilla vulgaris is demonstrated biologically active, no information about the root extract is available. This inspired us to start the current investigation into investigating A. vulgaris methanolic root extract's cytotoxic activity against various cancer cell lines in vitro, as well as its antimicrobial activity and phytochemical testing. MTT assay was used to determine the cytotoxic response against the prostate, breast, and colorectal adenocarcinoma, as well as normal Vero cells. In PC-3 cells, flow cytometry was used to determine cell cycle arrest and apoptosis vs. necrosis. In a dose-dependent manner, the obtained results showed strong antiproliferative activity on three cancer cell lines and the normal Vero cells. These results provide concrete evidence regarding the promising proapoptotic effects of A. vulgaris root extract's medicinal plant extract, which aids in the initiation of larger and in-depth future investigations.
Source link: https://doi.org/10.3390/plants11162140
DPT-1 and DPT-2 were shown to be pro-apoptotic agents in lung cancer A549 and non-small lung carcinoma H1299, ranging from IC50 = 1. 52-u201312. 89 u00b5 million, with a protective value to healthy cell lines BEAS-2B and NHDF in this study. The cell cycle's pro-apoptotic and pro-necrotic role of the investigated diazaphenothiazines was shown by cell cycle analysis by flow cytometry using Annexin-V assay. With a different mechanism and at a different rate, the investigated cancer models' biological reaction was evident in the investigated cancer models.
Source link: https://doi.org/10.3390/molecules27165255
Human beings' liver is one of the body's most vital organs. Hepatocellular Carcinoma is referred to as malignant hepatoma in 85% of all liver cancers, with the inability of drugs in subsequent stages making it lethal. Several ongoing research on HCC cell lines aid in understanding gene expressions, multiple signaling pathways, and different drug responses to HCC. This review article gives an insight into the possible molecular pathways involved in the emergence of commonly used HCC cell lines and the signals that are functionally active in them.
Source link: https://doi.org/10.52768/jjgastro/1040
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