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A new imaging method-- named dynamic nuclear polarization carbon-13 magnetic resonance imaging-- probes the glycolytic change in a real-time, dynamic manner. Complying with irradiation, the pyruvate-to-lactate conversion rates on DNP 13 C-MRI were substantially reduced in the hmc3 and the fadu cells but increased in the THP-1 cells. Western blot analysis validated the similar patterns in LDHA and LDHB expression levels.
Source link: https://doi.org/10.3390/metabo11080518
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