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The aim of this study is to determine which certain B cell subset is/are in charge of the production of safety IgM, IgG2 and IgA anti-pneumococcal capsular polysaccharides in response to immunization of healthy and balanced individuals with the Pneumovax, a paradigmatic T-independent type 2 vaccine. To resolve this inquiry, it will be made the most of the one-of-a-kind Ig heavy chain VDJ trademark shared by each B cell duplicate and the approach will depend on the search of clonal filiations in between plasmablasts/ plasma cells and various B cell subpopulations. Beginning with these blood samples, various B cells subsets and the PB/PC coming to a head at day 7 after vaccination will be isolated by cell sorting. When VDJ signatures of capPS will be verified, with this effective molecular tool, the private investigators will try to find the visibility of these trademarks with VDJ-specific PCR on cDNA prepared from the different B cell subsets.
Source link: https://clinicaltrials.gov/ct2/show/NCT02126384
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