Advanced searches left 3/3

COMPLEMENT - Springer Nature

Summarized by Plex Scholar
Last Updated: 03 July 2022

* If you want to update the article please login/register

Complement test for distinctiveness, uniformity and stability testing of kharif onion (Allium cepa L.) varieties

Identite, similarity, and stability testing is vital for plant variety identification and protection. The genetic diversity of ten onion species was determined by fourteen pairs of SSR markers and twenty-four morphological characteristics for the DUS test. The morphological characteristics of the onion varieties varied widely, which was useful in distinguishing the onion varieties. The onion varieties were divided into four major clusters containing a genetic similarity matrix ranging from 0. 52 to 1. 0, according to the estimated genetic similarity matrix based on molecular markers similarity coefficient. SSR markers' average polymorphism information content was 0. 64 percent, ranging from 0. 43 to 0. 89. A strong correlation was found between the two diversity tests of SSR markers and morphological characteristics, demonstrating a strong correlation. Therefore, the complement tests are particularly useful for varietal identification and security of varietal by reducing resource cost and time with high specificity.

Source link: https://doi.org/10.1007/s10722-022-01372-z


Serum neurofilament light chain, contactin-1 and complement activation in anti-MAG IgM paraprotein-related peripheral neuropathy

Both in routine practice and in clinical trials, there is a lack of reliable biomarkers to choose patients eligible for therapy and monitoring medical effects in anti-myelin-associated peripheral neuropathy in anti-myelin-related peripheral neuropathy. Both the Neurofilament light chain and contactin-1 can be used as indicators of axonal and paranodal damage. Complement activation is involved in anti-MAG PN pathogenesis. We therefore expected that serum NfL, CNTN1, C3b/c, and C4b/c may be biomarkers of disease activity in anti-MAG PN. Methods We included 24 treatment-nausefve patients with anti-MAG PN with IgM paraproteinemia, a high IgM MAG-antibody, and a neurophysiologist specialising in peripheral nerve disorders in this prospective cohort study. In anti-MAG PN patients, serum NfL, CNTN1 levels were mostly normal and were comparable to non-PN patients.

Source link: https://doi.org/10.1007/s00415-022-10993-4


Crosstalk between the renin–angiotensin, complement and kallikrein–kinin systems in inflammation

A variety of mediators influence physiological responses in inflammatory and infectious diseases, including renin'u2013angiotensin, complement, and kallikreinu2013kinin systems. This paper explores the interaction of the renin's tensin system, the complement system, and the closely linked kallikreinu2013kinin and contact systems in cell biological functions such as vascular tone and leakage, inflammation, thrombosis, and cell proliferation. During hereditary angioedema, cardiovascular and renal glomerular disease, vasculitides, and COVID-19, specific attention is given to the role of these systems in systemic inflammation in the vasculature and tissues.

Source link: https://doi.org/10.1038/s41577-021-00634-8


Edwardsiella tarda TraT is an anti-complement factor and a cellular infection promoter

TraT also interacted with host CD46 in a specific complement control protein domain-dependent manner, aiding in E. tarda-related cell infection and tissue disperation. TraT plays a vital role in E. tarda's complement evasion and systemic infection by virtue of being both an anti-complement factor and a cell infection promoter. Despite the host's strong resistance to killing by the host, Edwardsiella tarda TraT promotes cell infection and acts as an anti-complement agent, providing insight into the source of E. tarda u2019s strong host resistance to killing.

Source link: https://doi.org/10.1038/s42003-022-03587-3


Automated gathering of real-world data from online patient forums can complement pharmacovigilance for rare cancers

We're the first to investigate the benefits of patient forums for rare cancers. To this end, we conduct a case study on a patient registry for Gastrointestinal Stromal Tumor patients. Patient forum results can help determine which ADEs affect the quality of life the most, for several reasons, including cognitive impairment and alopecia as side effects of avapritinib.

Source link: https://doi.org/10.1038/s41598-022-13894-8


Complement C3a activates astrocytes to promote medulloblastoma progression through TNF-α

About Medulloblastoma is the most common malignant brain tumor in children. About one-third of MB patients are incurable. astrocytes contribute to the tumor microenvironment of MB and play a crucial role in MB progression, according to our previous studies. Methods: Using Math1-Cre/Ptch1 mice, which spontaneously produce MB, primary MB cells and astrocytes were isolated and then subjected to administration and coculture in vitro. By immunofluorescent staining, MB cell proliferation was determined. C3a-stimulated astrocytes were tested for GFAP and cytokine expression by immunofluorescent staining, western blotting, q-PCR, and ELISA. C3a receptor and TNF-u03b1 receptor expression was determined by PCR and immunofluorescent staining. By western blotting, we were able to activate the p38 MAPK pathway. To explore their role in MB proliferation in vivo, transplanted MB mice were treated with a C3a receptor antagonist or TNF-u03b1 receptor antagonist to investigate their role in MB progression. Conclusions We discovered that complement C3a, a portion released from intact complement C3 following complement activation, was enriched in both human and murine MB tumor tissue, and its receptor was highly expressed on tumor-associated astrocytes. We reported that C3a stimulated astrocytes and promoted MB cell proliferation via the p38 MAPK pathway, as shown in Figure 1. In fact, we found that administering a selective C3a receptor or TNF-u03b1 receptor antagonist to mice subcutaneously transplanted with MB slowed tumor formation in vivo. C3a was born during MB development. These results indicate that targeting C3a and TNF-u03b1 may be a promising novel therapeutic strategy for human MB.

Source link: https://doi.org/10.1186/s12974-022-02516-9


Exploring complement-dependent cytotoxicity by rituximab isotypes in 2D and 3D-cultured B-cell lymphoma

Trypan blue exclusion test was used to determine lymphoma cells grown in two and three-dimensional culture media systems. The anti-CD59 antibody was used to determine the presence of CD59 in RTX-mediated CDC responses. In lymphoma cells with a higher CD20/CD59 expression ratio, there was a rise in CDC activity. These lymphomas were also vulnerable to RTX when growing in 3D spheroids, but CDC presence was much reduced compared to 2D cultures. Conclusions The results reveal that the results of CDC lymphoma in CD20+ B-cell lymphoma is synergistically influenced by the choice of RTX isotype, antigen density, tumor structure, and degree of CD59 expression. Such as the CD20/CD59 ratio, for example, can help to determine RTX's CDC effectiveness in vivo and may help to produce standardized mAbs to raise patient response rates.

Source link: https://doi.org/10.1186/s12885-022-09772-1


A possible role of classical complement pathway activation in the pathogenesis of immunoglobulin G nephropathy: a case report

Immunoglobulin G nephropathy, a rare group of illnesses characterized by IgG deposits in the mesangial zone, is a unique group of disorders. However, IgG nephropathy is chronic as a single disease entity, and its mechanism remains to be elucidated. We explore a case of IgG nephropathy in which we observed activation of the classical complement pathway in the present case. Nephrotic syndrome had been ruled out of our hospital by a 47-year-old woman. The second generation of non-organized electron-dense deposits with a similar pattern of distribution were also found in this study, which were followed by foot process effacement. The IgG deposits were polyclonal and made up mainly of the IgG1 and IgG3 subclasses, according to an additional immunological examination. The present findings show that the pathogenesis of IgG nephropathy in our patients may have involved activation of the classical complement pathway.

Source link: https://doi.org/10.1007/s13730-022-00710-5


Complement C3 deficiency alleviates alkylation-induced retinal degeneration in mice

Methods Following treatment with the alkylating agent methyl methane sulfonate, C3 mRNA and protein levels were determined, DNA contamination, and photoreceptor cell death were determined in both wild-type C57BL/6J and C3 knockout mice. Following MMS use in C3 KO mice, the mRNA levels of nuclear erythroid-related factor 2 and related genes were higher. In the context of alkylation-induced retinal degeneration, our study found that C3 KO promotes photoreceptor cell survival and activates the Nrf2 signaling pathway.

Source link: https://doi.org/10.1186/s40662-022-00292-4

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions