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CELL Function - Crossref

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Last Updated: 10 May 2022

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Islet-1 Is Essential for Pancreatic β-Cell Function

In most adult pancreatic endocrine lineages, Isl-1 is present; however, its specific role in the postnatal pancreas is unclear. In postnatal -cells, Ablating Isl-1 reduced glucose tolerance without significantly reducing -cell mass or increasing apoptosis. We integrated high-throughput gene expression and Isl-1 chromatin occupancy using islets from Isl-1L/L; Pdx1-CreERTm mice and TC3 insulinoma cells, respectively, to identify direct targets of Isl-1. We discovered that Isl-1 directly occupies functional regulatory elements of Pdx1 and Slc2a2's functional regulatory frameworks using chromatin immunoprecipitation sequencing and a luciferase reporter assays.

Source link: https://doi.org/10.2337/db14-0096


The immunomodulatory function of human amniotic fluid stromal cells on B lymphocytes

The majority of current treatments for B cell-mediated disease are based on global B cell depletion, thereby removing pathogenic B cells as well as Breg subsets. Objective: On two subpopulations of B lymphocytes, different subpopulations of B lymphocytes will investigate the immunomodulatory function of human amniotic fluid stromal cells. In vitro, B lymphocytes were tested in vitro after coculture of B lymphocytes with hAFSCs, activation, proliferation, differentiation, cell cycle, and expression of the inhibitory costimulatory proteins B7H1, B7H3, and B7H4 were investigated in vitro. Inactivated B lymphocytes, coculture with hAFSCs resulted in a reduced number of memory B and plasma cells, as well as reduced amounts of immunoglobulins. The activated B lymphocytes could be susceptible to the expression of the negative co-inhibitory molecule B7H4 and PD-L1. These may be due to their effects on B cell apoptosis, cell cycle, and the expression of costimulatory molecules in human B lymphocytes.

Source link: https://doi.org/10.26599/jnr.2018.9040010


Orai3 and Orai1 are essential for CRAC channel function and metabolic reprogramming in B cells

Abstract The primary role of store-operated Ca 2+ channels in T cells is well understood, as shown by Ca 2+ release-activated Ca 2+ channels in T cells. Individual Orai isoforms' contributions to SOCE and their downstream signaling functions in B cells, on the other hand, are poorly understood. We find that Orai3 and Orai1 are vital components of native CRAC channels in B cells and are crucial for primary B cell proliferation and survival.

Source link: https://doi.org/10.1101/2022.05.06.490918


Hepatic ABCA1 Expression Improves β-Cell Function and Glucose Tolerance

Hepatic ABCA1 is the rate-limiting protein in HDL biogenesis, and mice lacking hepatic ABCA1 have extremely low plasma HDL levels. We used ABCA1-l/l mice, which showed decreased glucose tolerance and -cell function in glucose tolerance and -cell function, which had no changes in insulin sensitivity, but not in insulin sensitivity. WT and ABCA1-l/-l mice fed a high-fat diet had no difference in glucose tolerance or insulin secretion, and serum from ABCA1-l/l and WT mice fed a high-fat diet did not influence insulin secretion differently.

Source link: https://doi.org/10.2337/db14-0548


Residual Beta-Cell Function in Long Duration Type 1 Diabetes (T1D)

Participants from the DCCT/EDIC trial with an average of 35 years T1D duration were examined for residual beta cell function. Based on a previous DCCT report that showed that the risk of microvascular disease progression was dramatically higher among participants with a peak C-peptide concentration > 0. 03 nmol/L, we established a significant post-stimulus response as a result of a reading > 0. 03 nmol/L. Responders had lower HbA1c values and increased stimulated C-peptide at the DCCT baseline, and had lower insulin requirements both at the DCCT baseline and in the DCCT follow-up, which were lower insulin requirements at both baseline and later. In conclusion, beta cell function persists in some long-lived T1D patients and is associated with clinically meaningful reductions in severe hypoglycemic events.

Source link: https://doi.org/10.2337/db18-1686-p


The Incidence, Outcomes, and Risk Factors of Secondary Poor Graft Function in Haploidentical Hematopoietic Stem Cell Transplantation for Acquired Aplastic Anemia

Secondary poor graft function raises the risk of life-threatening complications following hematopoietic stem cell transplantation. In a haploidentical HSCT for acquired aplastic anemia patients, the prevalence, clinical outcomes, and risk factors of sPGF have not been clarified. We retrospectively reviewed 423 consecutive AA patients who underwent haplo-HSCT between January 2006 and December 2020, revealing a 3-year cumulative risk of 4. 6 percent of sPGF. To clarify the risk factors for sPGF, 17 sPGF cases and 382 without PGF were further investigated. The 2-year overall survival was significantly poorer for sPGF patients compared to those without PGF.

Source link: https://doi.org/10.3389/fimmu.2022.896034

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions