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Bronchitis - DOAJ

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Last Updated: 10 August 2022

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Infectious Bronchitis Coronavirus: Genome Evolution in Vaccinated and Non-Vaccinated SPF Chickens

Despite the use of many live IBV vaccines around the world, the chicken industry continues to suffer significant economic losses due to the infection of Infectious Bronchitis virus. Three passages of a challenge IBV in SPF chickens were either inoculated or vaccinated at the same time of age using a live vaccine heterologous to the challenge virus in order to investigate this hypothesis. Virus-neutracous viruses were found in all birds that had been exposed to vaccine heterologous virus.

Source link: https://doi.org/10.3390/v14071392


A Novel Nanobody-Horseradish Peroxidase Fusion Based-Competitive ELISA to Rapidly Detect Avian Corona-Virus-Infectious Bronchitis Virus Antibody in Chicken Serum

The bronchitis virus, which has caused significant injury and economic damage to the global poultry industry, is a bacteriae virus associated with Avian coronavirus-infectious bronchitis. We developed a novel nanobody against IBV-N protein for the manufacture of a rapid, simple, affordable, and specific competitive ELISA for IBV antibody determination in order to allow the assessment of infection origins and early warning of disease transmission. The novel cELISA for IBV antibody detection is a simple, quick, specific immunoassay, and it can be used to quickly test IBV antibody contributing to disease prevention and control.

Source link: https://doi.org/10.3390/ijms23147589


Bacterial artificial chromosome-based reverse genetics system for cloning and manipulation of the full-length genome of infectious bronchitis virus

We developed a reverse genetics device for cloning the full cDNA of the IBV C-78E128 attenuated strain in a bacterial artificial chromosome in this paper in order to facilitate the molecular analysis of IBV pathogenesis and the creation of a new-generation IBV vaccine. The resultant S gene recombinant virus was found to be extremely infectious and capable of generating a serotype-specific VN antibody against the S95 strain, but the S gene recombinant virus did not fully recapitulate the S95E4 strain's tissue tropism. The IBV BAC-based RGS, which allows cloning and manipulation of the IBV virus genome entirely in E. coli, provides a useful platform for molecular studies of IBV pathogenesis and the manufacture of well-designed IBV recombinant vaccines.

Source link: https://doi.org/10.1016/j.crmicr.2022.100155


Molecular Characterization Analysis of Prevalent Infectious Bronchitis Virus and Pathogenicity Assessment of Recombination Strain in China

The Avian coronavirus-infectious bronchitis virus is a respiratory pathogen of chickens, resulting in significant economic losses in the poultry industry. In eight provinces of China, this study sought to monitor and isolate IBV in broiler flocks with respiratory diseases. Using PCR assay, 910 samples from broiler flocks had IBV positive rates of 17. 6%. The CK/CH/JS/YC10-3 isolate most likely originated from recombination events between strain YX10 and strain TW2575-98, the pathogenicity of which was determined, comparing strain YX10 and strain TW2575-98 was tested, comparing it to strain TW2575-98 and strain YC10, comparing it to strain TW2575-98 and strain CK/CH/GD/JR07-7.

Source link: https://doi.org/10.3389/fvets.2022.842179


Establishment and Cross-Protection Efficacy of a Recombinant Avian Gammacoronavirus Infectious Bronchitis Virus Harboring a Chimeric S1 Subunit

The T248S swap, according to further research, may be important for the migration of the recombinant virus to cell culture. Strong antibody responses were evoked by the recombinant IBV-infused chicks, which showed strong cross-protection against threats from virulent M41 and a QX-like genotype IBV. This research reveals the possibility of releasing rIBV-Beau-KC as a cell-based vaccine with a broad protective shield against two different genotypes of IBV.

Source link: https://doi.org/10.3389/fmicb.2022.897560


Novel and Alternative Therapeutic Strategies for Controlling Avian Viral Infectious Diseases: Focus on Infectious Bronchitis and Avian Influenza

The growth of poultry raising has resulted in the introduction of infectious diseases and their pathogens in various species of birds, such as avian infectious bronchitis virus and avian influenza virus. IBV and AIV are two common causes of poultry mortality and economic loss. Antiviral drugs that are often used often result in virus resistance, recurrence, and latency of viruses. The pharmaceutical industry is gradually focusing on plant extracts in order to produce novel herbal drugs with proper antiviral characteristics. The virus entry and replication of viruses in the host and the prevention of viral pathogenesis have been detailed by the authors. Nanotechnology is one of the recent technologies that can be very useful in diagnosing and controlling infectious diseases in poultry.

Source link: https://doi.org/10.3389/fvets.2022.933274


Genome Sequence Variations of Infectious Bronchitis Virus Serotypes From Commercial Chickens in Mexico

Despite regular vaccinations, new strains of infectious bronchitis viruses are also emerging. Here, we report the genetic variations of IBVs resulting from random non-targeted next generation sequencing of vaccine and field samples collected on FTA cards from commercial flocks in Mexico in 2019u20132021. In 60. 07% of the tested samples, from which 33 complete genome sequences were de novo assembled, IBV RNA was discovered in 60. 07% of the tested samples, with which 33 complete genome sequences were de novo assembled. This report shows the use of FTA cards as a cost-effective, widely used low-cost sampling method for untargeted discovery of avian viral agents in field-collected clinical data. Our research contributes to multiple distinct IBVs in Mexican commercial flocks co-circulation, underscoring the need for active surveillance and a study of IBV vaccines currently used in Mexico and the greater Latin America region.

Source link: https://doi.org/10.3389/fvets.2022.931272

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions