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By providing an in vitro-like early stage evaluation of efficacy and toxicity, three-dimensional cell cultures have a great chance of lowering costly late-stage drug losses. This transglutaminase crosslinked gelatin scaffold provides a native ECM scaffold for cell-cell and cell-matrix interaction, and its transparency aids routine observation with ease; the proteolysis sensitive scaffold supports cell migration and invasion; and micrix rigidity mimics physiological and pathological environments. Tgel is made from well identified ingredients, a wide variety of handling characteristics, fast to prepare at room temperature, and little batch to batch variation relative to Matrigel. We make 3D cultures more relevant by including two or more cell types in various co-cultures. Viable cells can be easily removed from the 3D T-gel after the test, which can be used for further experimentation. In nude mouse models, T-gel can also be used as a carrier for xenograft tumor formation.
Source link: https://doi.org/10.1158/1538-7445.am2013-3833
1 technological advancements in the in situ analysis of lung tissue, 2 new insights into stem cell signalling and plasticity in lung remodelling and regeneration, 3 the role of extracellular matrix in stem cell regulation and airway engineering in lung regeneration, 5 differentiating and delivering stem cell therapeutics in lung regeneration, 6 ethical aspects of cell-based therapies for lung diseases are among the topics covered: 1 technological advances in lung reconstruction and regeneration, 5 regeneration in the lung regeneration, 6 ethical research of cell-based lung diseases The conference concluded with an open discussion to identify funding priorities and recommendations for future research directions in basic and translational lung biology.
Source link: https://doi.org/10.1152/ajplung.00113.2022
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