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Bacteria - Europe PMC

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Last Updated: 27 September 2022

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Bdellovibrio lyse multiple pathogenic bacteria and protect crucian carp Carassius auratus gibelio from Aeromonas veronii infections

Bdellovibrio are obligate defenders of Gram-negative bacteria that can parasitize other bacteria and cause lysis. The Aeromonas hydrophilia was used as the host bacteria for cultured Bdellovibrio in order to better understand the Bdellovibrio's inhibitory activity against common aquatic pathogens and its potential use in aquaculture. The amount determination of Bdellovibrio sp. was also determined by an absolute qPCR detection system with specific primers. Bdellovibrio sp. , cultured Bdellovibrio sp. morphological features of the cultured Bdellovibrio sp. The infection with A. vironii can cause significant mortality in Carassius auratus caused by waterborne viruses. In addition, the Bdellovibrio sp. amount has decreased, although it is likely that the number of Bdellovibrio sp. intestines and gills of C. auratus could rise with time after treatment, meaning that Bdellovibrio may have colonized in fish. Bdellovibrio can play an important role in protecting aquatic animals from pathogenic bacteria, according to the present study.

Source link: https://europepmc.org/article/MED/IND607900030


Effect of environmental factors on the kinetics of evaporation of droplets containing bacteria or viruses on different surfaces

Standardised testing procedures can be used to determine antimicrobial surfaces at solid-air interfaces, such as dropping a droplet inoculated with microorganisms onto the surface and monitoring changes in microbial counts over time. The droplet evaporation time was measured on four separate surfaces, where temperature, relative humidity, and airflow in the test chamber were controlled. The droplet profile/contact angle remains unchanged; and a mixed mode model were compared with simple models based on external mass transfer for forecasting the evaporation time.

Source link: https://europepmc.org/article/MED/IND607818534


Inhibition of biofilm formation of Geobacillus stearothermophilus in calcium-reduced milk protein formulation is associated with calcium, sodium and bacteria growth history: A preliminary study

Three G. stearothermophilus strains were tested in two milk formulations over 18 h, but MF1 and MF2 had similar compositions, but MF2 had elevated sodium and low calcium levels in comparison, but MF2 had relatively high sodium and low calcium levels. The cationic quality of MF2 in reducing biofilms of G. stearothermophilus was evident in reducing biofilm formation of A1 at 18 h and P3 at 10 h.

Source link: https://europepmc.org/article/MED/IND607537979


Directed Evolution of Fluorescent Proteins in Bacteria.

In E. coli, we present a novel strategy for the controlled evolution of a far-red fluorescent protein. For a second small-molecule fluorophore, biliverdin, the new method used genes to produce fluorophores inside E. coli and allowed for the substitution of the native fluorophore, phycocyanobilin. Eventually, the evolution introduced fluorescent proteins that expressed in large amounts in E. coli. The transformed fluorescent protein was identified as the small ultra-red fluorescent protein and was biophysically as brilliant as the new green fluorescent protein. This chapter discusses the materials and processes used to produce a far-red fluorescent protein in bacteria.

Source link: https://europepmc.org/article/MED/36107338


In vitro digestion, fecal fermentation, and gut bacteria regulation of brown rice gel prepared from rice slurry backfilled with rice bran

This research was conducted to see if brown rice gel, which was made by backfilled rice slurry with rice bran, was capable of altering the gut bacteria. BRG may cause starch formation, reduce viscosity, and breakdown, decrease the glycemic index of in vitro digestion from 85. 79 to 72. 76 percent due to the presence of rice bran, compared to rice gel. BRG promoted the manufacture of short-chain fatty acids after 24 h of fermentation, with the amounts of acetic acid, propionic acid, and butyric acid up to 10. 84 bcg/mL, respectively. The findings will make it possible to use whole-grain brown rice in gel-based functional foods.

Source link: https://europepmc.org/article/MED/IND607837673


MCR-1-dependent lipid remodelling compromises the viability of Gram-negative bacteria.

The withdrawal of colistin as a feed additive dramatically reduced the incidence of mcr-1 among our group and others, according to new studies published by our company and others. The detailed mechanism of MCR-1's decline may have been unclear, although it is acknowledged that the rapid decline in mcr-1 prevalence may have arisened, to a certain extent, from MCR-1's toxic effects. MCR-1 destroyed the outer membrane permeability in Escherichia coli and Klebsiella pneumonia, and this event was associated with MCR-1-mediated cell shrinkage and death during the stationary phase. Notably, MCR-1-expressing cells' capacity for recovery from the stationary phase under improved conditions was reduced in a time-dependent manner. PbgA, a sensor of periplasmic lipid-A and LpxC production that took place in the first step in lipid-asis, was reduced during the stationary phase, suggesting that MCR-1 disrupted lipid homeostasis.

Source link: https://europepmc.org/article/MED/35437117


Evaluation of antimicrobial compounds to inhibit growth of select Gram-positive pathogenic or antimicrobial resistant bacteria in air-exposed silage

The ability of some antimicrobial additives in air-exposed silage was determined using pure and mixed bacterial cultures. In Listeria monocytogenes, laurate, and monolaurin, there are decreases of 4 to > 7 logs/u2080;u2080 colony-forming units, u00b9, compared to controls. According to u2080B's 4 and 24 h incubations, respectively, titropropanol decreased E. faecalis and L. monocytogenes 2. 9 percent and 2. 4 log u20137. 2 log u2080u20137. 2 logs. Tests of carry-over effects against ruminal microbes revealed laurate, monolaurin, and 2-nitropropanol inhibited methanogenesis by more than half after 24 hours of incubation and reduced L. monocytogenes and enterococci.

Source link: https://europepmc.org/article/MED/IND607690084


Bacteria-bacteriophage cycles facilitate Cholera outbreak cycles: an indirect Susceptible-Infected-Recovered-Bacteria- Phage (iSIRBP) model-based mathematical study.

Cholera is an acute enteric infectious disease caused by the Gram-negative bacterium Vibrio cholerae. A human race, a bacterial population, and a phage population are all represented in the compartmental model. There could be eight equilibrium points, one of which is a disease-free equilibrium point, according to our estimates. In addition, we explore the main implications in terms of public health surveillance and control policies.

Source link: https://europepmc.org/article/MED/34994295


MALDI-TOF MS for rapid detection and differentiation between Tet(X)-producers and non-Tet(X)-producing tetracycline-resistant Gram-negative bacteria.

The widespread use of tetracycline-resistance genes in Gram-negative bacteria has resulted in the proliferation of tetracycline-resistance genes in Gram-negative bacteria, posing serious dangers to human and animal health. Test strains were incubated with tigecycline and oxytetracycline in separate tubes for 3 hours for 3 hours before determining spectral peaks of tigecycline, oxytetracycline, and their metabolite. Interestingly, we also discovered that the reactive oxygen species produced by tetracycline-susceptible strains were able to promote oxytetracycline degradation. Overall, the MALDI Tet-plus test is a fast and easy way to find Tet-producers, non-Tetracycline-resistant strains, and tetracycline-susceptible strains.

Source link: https://europepmc.org/article/MED/34951562

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions