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Bacteria - BioProject

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Last Updated: 27 September 2022

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Suppressor mutant screening lacking growth-essential tRNA modification in bacteria

A conserved methyltransferase TrmD-grown tRNAs in bacteria, resulting in bacterial growth, with the m1G37 version on a subset of tRNAs in bacteria. Here we performed suppression screening to isolate a mutant E. coli cell that lacks TrmD but is viable, and the whole-genome sequencing revealed several mutations on prolyl-tRNA synthetase gene conferring cell viability in the absence of TrmD.

Source link: https://www.ncbi.nlm.nih.gov/bioproject/883896


Epiphytic bacteria, Ulva lactuca, culture-based bioprospecting, genome of a producer bacteria of laccases

In this sense, the epiphytic bacterial colonies of U. lactuca, a species of epiphytic bacterial communities, are a useful source of novel enzymes produced by the bacteria to metabolize various substrates provided by the macroalgal surface in this sense. Functional testing of two enzymes was carried out in this research on 90 epiphytic bacteria isolated from the surface of U. lactuca's soil, which was collected in La punta de la loma, Santa Marta. A total of 24 isolates were tested positive for laccase activity in three substrates ranging from Bacillus, Brevibacterium, Lysinibacillus, Pseudomonas, Shewanella, Vibrio, Alcaligenes, and Achromobacter. Using two gene products, which may be responsible for the laccase activity found in plate, were discovered to have the ability of epiphytic bacteria of U. lactuca in the production of enzymes, which may be unique to modern lacases made by terrestrial fungi.

Source link: https://www.ncbi.nlm.nih.gov/bioproject/882333


Leaf associated bacteria and fungal communities in intermittent streams

Sampling of experimentally placed leaf bags had over six collection dates at seven locations in the Western Italian Alps with historically poor streamflow conditions. Chestnut and oak leaf tissue contained distinct bacterial and fungal communities, with a number of taxonomic groups present in abundance, although bacterial community structure converged later in decomposition. Compared to leaf type and collection date, historical flow conditions and observed conditions had relatively little effect on bacterial and fungal populations. Our results show the importance of leaf characteristics to leaf litter in-stream conditioning and the need for further investigation of drying events in Alpine streams. This research gives new insight into leaf litter decomposition with predicted flow changes associated with a global change scenario.

Source link: https://www.ncbi.nlm.nih.gov/bioproject/882013


Whole genome sequencing of isolates of Mycobacterium ulcerans to trace effectively the evolutionary history of the bacteria and understand its dissemination and evolution at a local scale (villages).

A large number of clinical isolates must be sequenced in a single endemic region in order to investigate M. ulcerans' dissemination and evolution at a local level. We first published a phylogeographic review of 207 isolates obtained from human clinical samples spanning ten years and identifying two endemic departments in Benin and one neighboring department in N. . . . ia in 2020.

Source link: https://www.ncbi.nlm.nih.gov/bioproject/881288

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions