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pH 7 is adjusted to pH 7 and placed on a column of Dowex-1 u00d7 8 previously equilibrated with 0. 1 M acetate buffer at pH 4. Acids Glutamic and aspartic acids are separated by elution with 0. 1 M acetate buffer at pH 4. 0-u20134. 6. At pH 5. 5u20135. 9. 9, the etonic acid is eluted with 0. 2 M acetate buffer at pH 5. 5u20135. 9.
Source link: https://doi.org/10.1139/y57-143
The complete intramolecular distribution of C 14 has been determined by measuring Glutamic acid, aspartic acid, and threonine isolated from wheat plant's gluten, which was administered during growth. Developed glutamic acid from acetate-C 14 was in carbon-5 and 20% in carbon-1; glutamic acid from acetate-2-C 14 contained 43% of the total carbon-14 and 18% in each of carbon 2 and 3. Acetate-1-C 14 resulted in labelling primarily in aspartic acid's terminal carbons, and acetate-2-C 14 preferentially identified the internal carbons.
Source link: https://doi.org/10.1139/y57-044
Invertase is a commercially important enzyme used in the food industry to produce invert sugar, which is composed of a blend of equal amounts of glucose and fructose. According to the findings, 104. 1 mg g u22121 and 135. 5 mg g u22121 of adsorption capacity values were found for cryogel and magnetic microparticle forms, respectively. The activity of invertase immobilized on polymeric structures was maintained at an annual rate of 83. 6% for the particle form and 89. 2% for the cryogel form, according to the publisher.
Source link: https://doi.org/10.1515/polyeng-2021-0373
Despite the dramatic decrease in viability over time, 0. 002 percent of P. furiosus cells and 0. 2 percent of T. litoralis cells remained viable after 25 and 50 days, respectively. The D/L Asp ratio in the starved cells was about half of those from the autoclaved cells, indicating that the starving cells were capable of partially rebuilding racemized Asp. The gene encoding Protein-L-isoaspartate O-methyltransferase in the repair of damaged proteins by returning D-Asp back to L-Asp during cell resuscitation, according to a transcription of starving cells.
Source link: https://doi.org/10.1093/femsec/fiab112
Abstract Poly hydrogel is a biodegradable and biocompatible polymer with high water absorption capacity. The cross-linkers in this paper investigate two new ways to prepare PASP hydrogel without organic solvent by using hydrazine hydrate and ethylene glycol diglycidyl ether or poly diglycidyl ether as the cross-linkers. Preparing PASP hydrogel in aqueous solution is promising and finds its use in several applications.
Source link: https://doi.org/10.1515/polyeng-2014-0275
Podarcis s. sicula's current research examined the role of D-aspartic acid in ovarian steroidogenesis and its effect on aromatase production in the lizard, D-aspartic acid. D-Asp administration resulted in a decrease in testosterone and an increase in oestradiol, according to the study, and that treating with other amino acids instead of D-Asp has no effect. Furthermore, these tests revealed an increase in aromatase activity as the addition of D-Asp either to fresh ovarian tissue homogenate or to acetonic powder of ovarian follicles led to a significant rise in testosterone conversion to oestradiol.
Source link: https://doi.org/10.1530/rep.0.1210803
OBJECTIVE: The stimulatory and inhibitory effects of N-methyl-D-aspartic acid and kainic acid on prolactin secretion have been linked to serum prolactin concentrations before drug delivery. We investigated the role of NMDA and kainic acid in PRL secretion in females with different serum concentrations of PRL in the current experiments. We investigated the role of dopamine in NMDA's effects, as well as the effects of pretreatment with domperidone. NMDA did not change PRL in neonatively androgenized females, although NMDA and kainic acid reduced PRL release in neonatively oestrogenized females. CONCLUSIONS: NMDA has two effects on prolactin secretion that are independent of prior prolactin concentrations and dopamine activity, but kainic acid is only inhibitory.
Source link: https://doi.org/10.1530/eje.0.1380460
According to the previous finding of a cysteine in a KSI-directed photoinactivation experiment of KSI, none of the cysteines in P. putida's KSI is necessary for catalytic function, contrary to the previous finding of a cysteine in an active-site-directed photoinactivation study of KSI. Asp-103 was discovered within the range of the hydrogen bond to the equilenin, as determined by X-ray crystallography at high resolution, based on three-dimensional models of KSIs with and without dienolate intermediate analog equilenin. The k value of the mutant KSI was 85-fold lower than that of the wild-type enzyme, but there was a modest decrease in the k value for the D103A mutant, which retained some hydrogen bonding capability; however, the k value of the mutant was still higher than that of the wild-type enzyme.
Source link: https://doi.org/10.1128/jb.179.24.7742-7747.1997
The pHLIP peptide is a new therapeutic and imaging agent for cancer therapy. The polar toxin phalloidin is translocated in a pH-dependent manner into HeLa, JC, and M4A4 cancer cells, inhibiting cell growth and inducing cytoeskeletal immobilization and multinucleation, according to the author. Here we investigate the determinants of pHLIP membrane insertion. We found a correlation between Asp's number and location with the peptides' ability to penetrate into and exit from the membrane in a pH-dependent manner. We also discovered that the number of Asp in the peptide determines the insertion characteristics, indicating that the tumor labeling characteristics of pHLIP can be tailored to better reflect solid tumor physiological acidity. In:: In: Proceedings of the Second AACR International Conference on Frontiers in Basic Cancer Research, 109-18; San Francisco, CA; 2011 Sep 14-18;.
Source link: https://doi.org/10.1158/1538-7445.fbcr11-c2
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