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Arthritis - Europe PMC

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Last Updated: 10 June 2022

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Effect of Er Miao San on peritoneal macrophage polarisation through the miRNA-33/NLRP3 signalling pathway in a rat model of adjuvant arthritis.

In a 1:1 ratio, Context Er Miao San is a tablet that contains Atractylodis Rhizoma and Phellodendri Cortex, and is commonly used to treat rheumatoid arthritis and other inflammatory diseases. In a rat model of adjuvant arthritis, we investigated the origins and effects of EMS on peritoneal macrophage differentiation. By flow cytometry, the ratio of CD86/CD206 in macrophages was determined. By ELISA, the cytokines in the serum and cell supernatants were determined. Results EMS significantly reduced the AA index in rats and pathological changes in the ankle joint. Besides, EMS treatment raised IL-10 and TGF-u03b2 levels in AA rats' serum and supernatant, as well as decreased the IL-1 and TNF-u03b1 levels, which were also reduced simultaneously. "Our findings reveal that EMS can reduce macrophage polarization to the M1inflammatory phenotype by downregulating the miRNA-33/NLRP3 pathway in AA rats," says the author.

Source link: https://europepmc.org/article/MED/35608068


Artificial intelligence-assisted development of in situ forming nanoparticles for arthritis therapy via intra-articular delivery.

"Artificial intelligence is playing an increasing part in the experimentation of experiments, making it an excellent tool for saving both time and money. " Accordingly, the aim of this research was to develop, characterize, and optimize target forming nano particles for IA distribution of piroxicam by using Design u00ae Expert as an AI-based system, where a 3 full scientific experimental design was adopted. And, as compared to the positive control, the optimized-ISNs' once-a-week IA administration resulted in a dramatic decrease in both STAT-3 and RANKL's protein expression, as well as a significant decrease in the content of IL-17, MMP-3, NF-u03bb1, and TNF-u03b1. In conclusion, the use of ISNs for intra-articular injection has demonstrated their effectiveness in piroxicam therapy for RA patients.

Source link: https://europepmc.org/article/MED/35532141


Targeted co-delivery biomimetic nanoparticles reverse macrophage polarization for enhanced rheumatoid arthritis therapy.

"The key cause of the disease is a mismatch between pro-inflammatory macrophages and anti-inflammatory macrophages in the joint's synovial tissue. " The accumulation of nanoparticles in the inflammatory joint site was higher, according to the study. In vivo, the real-time fluorescence imaging system and HPLC, was used to investigate nanoparticle distribution and pharmacokinetics, but the results revealed that the accumulation of nanoparticles in the inflammatory joint site was higher. To promote macrophage polarization in the M1-M2 region, the synergistic therapy effect of IL-10 pDNA and DSP in this system was enhanced by lowering the concealment of pro-inflammatory factors and increasing the expression of anti-inflammatory genes. Our goal is to co-deliver gene and chemical drugs by biomimetic natural materials in order to foster macrophage polarization in order to achieve synergically treatment of inflammatory disease.

Source link: https://europepmc.org/article/MED/35363114


Silk fibroin hydrogel containing Sesbania sesban L. extract for rheumatoid arthritis treatment.

"This report found novel silk fibroin in-situ hydrogel containing Sesbania sesban L. extract, a plant with high anti-inflammatory properties that is helpful in rheumatoid arthritis therapy. " Methods The hydrogels were made using a simple method of spontaneous gelation at different temperatures. With appropriate techniques, the gel properties of morphology, gelation time, viscosity, gel strength, stability, drug loading capacity, drug release rate, and in-vitro anti-inflammatory activity were investigated. Results The ideal formulation had a very porous structure, with a gelation time of 0. 5 h at room temperature and bodily temperature of 37 °C, a viscosity of 2530. 14 g, and a physical stability of > 6 months, with a gelation time of 0. 5 h at room temperature and bodily temperature of 37 u00b1 50 g. Moreover, the hydrogel contained the Sesbania sesban L. leaf extract with a total phenolic content of 92. 8 mg/g and remained the release rate for more than 20 d. u1ys, following the Higashi model. ".

Source link: https://europepmc.org/article/MED/35277106


Trans ethosomal hybrid composites of naproxen-sulfapyridine in hydrogel carrier: anti-inflammatory response in complete Freund's adjuvant induced arthritis rats.

According to good stability and polydispersity index, NAP and SULF ethosomes grew by an average vesicle size between 251. 1 nm and 269. 0 nm. " The entrapment effectiveness of NAP and SULF ethosomes was over 66%, and NAP-SULF ethosomes-hydrogel displayed a persistent release effect. With less paw volume on induced arthritis Albino Wistar rats, in vivo studies on NAP-SULF EH show significant reduction of inflammation. To sum up, a blended blend of NAP-SULF in a hydrogel carrier reduces inflammation efficiently, and it may be a pioneer for trans delivery of drugs in RA. ".

Source link: https://europepmc.org/article/MED/35261304


Delivery of germacrone (GER) using macrophages-targeted polymeric nanoparticles and its application in rheumatoid arthritis.

To prevent arthritis progression, the gastronutrient 1 cell/the T helper 2 cell balance can be controlled by the gastroenterone" or the T helper 2 cell ratio. " FA receptor-targeted nanocarriers were manufactured with GER to reverse M1-type proinflammatory cells and reduce inflammation. To reverse M1-type proinflammatory cells and reduce inflammation, M1-type proinflammatory cells and inflammation, FA receptor-targeting nanocarriers loaded with GER were manufactured. In vitro studies showed that FA-NPs/GER could promote the conversion of M1 macrophages into M2 macrophages. Pro-inflammatory cytokines in the rat's inflammatory tissue were significantly lower than those in other treatment groups, indicating a dramatic therapeutic effect in AIA rats. ".

Source link: https://europepmc.org/article/MED/35225122


Dextran sulfate-based MMP-2 enzyme-sensitive SR-A receptor targeting nanomicelles for the treatment of rheumatoid arthritis.

"The dextran sulfate was modified to produce nanoparticles for targeted delivery of Celastrol to rheumatoid arthritis and a specific drug type" by binding to macrophage's scavenger receptor. " The MMP-2 inflammatory peptide was used to crack in the inflammatory microenvironment to accelerate the release of Cel in the in vitro release study. DPC@Cel had better anti-rheumatoid arthritis benefits and reduced systemic toxicity than free Cel" in vivo studies, according to In vivo experiments.

Source link: https://europepmc.org/article/MED/35119317


The emerging role of irisin in experimentally induced arthritis: a recent update involving HMGB1/MCP1/Chitotriosidase I-mediated necroptosis.

"Objectives Necroptosis is a tightly controlled inflammation necrotizing cell death pathway that aids in the onset of specific diseases such as rheumatoid arthritis. Evaluation of irisin effectiveness as a novel therapeutic agent in experimentally induced RA by changing the immune-inflammatory, necroptotic molecular, and biochemical signaling pathways. Methods RA was initiated in 30 female Wister albino rats by a single subcutaneous injection of collagen-III with incomplete Freund's adjuvant and a booster immunization dose ten days later. For four weeks, 15 rats were treated by S. C irisin injection daily for 4 weeks. Results The irisin significantly reduced the level of all assessed biochemical parameters, except MDA, which was much lower in comparison to the correspondent values in the arthritic group with no treatment. "Irisin has therapeutic anti-inflammatory and antioxidant properties by modulating immuno-inflammatory, necroptotic molecular, and biochemical signaling pathways in rats experimentally induced RA. ".

Source link: https://europepmc.org/article/MED/35094663


Analytical Study of the Risk Factors Affecting the Fracture Risk in Rheumatoid arthritis Patients using FRAX Algorithm.

"Objectives" The present study was designed to determine the risk of osteoporotic fracture in patients of Rheumatoid arthritis by the Fracture Risk Assessment Tool and its relationship with osteoporotic-specific risk factors. In addition, we reviewed the FRAX software for all patients and controls in order to determine the 10-year risk of major osteoporotic fracture and hip fracture. The duration of disease, previous fracture, and a substantial risk of hip fracture in RA patients were all significantly higher in mean 10-year risk of major osteoporotic fracture, with significant hip fractures; parent fractured hip with significant osteoporotic fracture and hip fracture. Conclusions As determined by the FRAX system, both male and female patients with RA significantly increased the risk of hip fracture and major osteoporotic fractures. The duration of the disease, previous fracture, and parent fractured hip showed a strong association with significant osteoporotic fracture and hip fracture. ".

Source link: https://europepmc.org/article/MED/35057596


Comparison of the anti-inflammatory effects of different polar ginsenosides on rheumatoid arthritis

"Rheumatoid arthritis is a chronic autoimmune disease. " Traditional ethnic medicine has particular strengths in the treatment of such disorders. In a collagen-induced arthritis model based on ultra-performance liquid chromatography-mass spectrometry, the aim of this research was to elucidate the therapeutic effects of various polar ginsenosides on RA and to investigate the metabolic profiles of ginsenosides. Polar ginsenosides modified the urine chemistry with respect to catecholamine biosynthesis and vitamin B6 metabolism. This research has shown that less-polar ginsenosides can reduce inflammation symptoms to a greater extent than polar ginsenosides. ".

Source link: https://europepmc.org/article/MED/IND607716317

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions