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Aim To determine DEC and the antimicrobial resistance of ESBL-PE isolated among diarrhoea patients attending two health clinics in Ghana. Methods Stool samples were collected from 122 diarrhoeal patients who attended Maamobi General Hospital and Kaneshie Polyclinic between January 2019 and March 2020, with 122 diarrhoeal patients who attended Maamobi General Hospital and Kaneshie Polyclinic between January 2019 and March 2020. Conclusions: Enobacteriaceae was recovered from the patients in the study with an overall ESBL prevalence of 20. 4%, Klebsiella pneumoniae, 37. 3%, and Progeus mirabilis, 51%. The heat labile gene of Enterotoxigenic E. coli isolates isolated from Thirty-four E. coli isolates possessed the heat labile gene of Enterotoxigenic E. coli. Conclusion Our results support the presence of DEC and the antimicrobial resistance patterns of ESBL-PE among stool isolates, limiting the choices of commonly used drugs for diarrhoeal treatment in Ghana. For a better understanding of their epidemiology and clinical implications, Routine laboratory experiments in health care facilities and enhanced surveillance networks among hospital networks are encouraged.
Source link: https://doi.org/10.1371/journal.pone.0268991
In Brazil, there are no microbiological studies of reptiles. The study, which was published here, focuses on a species of snake of great medical concern and serves as a basis for other research and comparisons. Salmonella sp isolates (Sw. B. ) Fourteen serotypes of Salmonella were identified; 13 of the subspecies IIIb and one of the subspecies IV are among the subspecies IV. The most sensitive to the tested drugs were Kluyvera and Salmonella. Aminoglycosides had excellent antimicrobial activity, but chloramphenicol was the only drug to which no isolate demonstrated resistance. Outros efoca comparaes e compareae7u00ef5es. delu00e9cie de grandes interesse mu00e9es del aqui de descrito enfoca uma espoca de serpente de grande mu00e9cies de efoca O elti espargo e. U00e9dico e mo e mo e es MacConkey em u00e1gar XLT4 por O material foi semeado foi semeado em u00e1gar XLT4 u00e1gar MacConkey em u00e1gar MacConkey em om u00e1gar XLT4 em XLT4 X em e1gar O material semea1gar O material semeado e1gar Xe1gar em e1gar em X em e1gar em X em e em e1gar MacConkey e1gar XLT4 em e1gar em e XLT4 em e1gar em e1gar e de Salmonella sp. Os isolados de Salmonella sp. The subespu00e9cie IV has been established by Salmonella foram identifies, with 14 sorotipos de Salmonella foram identificados, as shown by the subespu00e9cie IIIb. Salmonella foram os gu00eaneros, but sensu00edveis u00e0s drogas testadas.
OBJECTIVE: In Bacteroides spp, this research investigated the antimicrobial resistance profile and the prevalence of resistance genes. The presence of resistance genes was determined by hydrolysis of the chromogenic cephalosporin of 114 Bacteriodales strains isolated from 39 children's fecal samples, and the presence of resistance genes was determined by using a PCR assay. In 92% of the tested strains, P-lactamase production was verified. CONCLUSIONS: Our findings show an increase in the resistance to several antibiotics in intestinal Bacteroides spp. Microorganisms from distasonis and Parabacteroides distasonis, with the discovery that these microorganisms have antimicrobial resistance genes that may be transferred to other susceptible intestinal strains.
Source link: https://doi.org/10.1590/S1807-59322011000400004
We prospectively tested a new molecular assay in 79 consecutive hematologic patients, sepsis due to gram-negative bacteria. A total of 82 gram-negative microorganisms were isolated by blood cultures, of which 76 percent were mono-microbial. The concordance with standard blood cultures was 100 percent, considering the bacteria that were detectable by the device. In 20 of the isolates, resistance genes were detected, and 100% were congenital with the phenotypic antibiotic resistance.
Source link: https://doi.org/10.2991/chi.d.190321.001
More than 30 varieties of artisanal cheeses are known in Brazil; however, microorganisms, such as Staphylococcus spp, can contaminate raw milk cheeses from various sources, from milking to processing. Enterotoxins have been produced and produced by Staphylococcal food poisoning after eating a diet rich in coagulase-positive staphylococci, mainly Staphylococcus aureus, as well as Staphylococcus aureus. In addition, the ability of Staphylococcus spp. Made with raw milk, Brazilian artisanal cheese is made with raw milk.
Source link: https://doi.org/10.3168/jds.2021-21634
ABSTRACT: Dairy cows can be reservoirs of foodborne pathogens such as Salmonella, which pose significant public health risks to humans. Salmonella Infantis was the most common serovar, followed by Salmonella Kiambu and one isolate of Salmonella Cerro, according to the authors. Against streptomycin, the highest antimicrobial resistance prevalence of Salmonella isolates was found against streptomycin, ceftriaxone, and chloramphenicol. Salmonella isolates with two resistance genes: aac gene that gives protection against aminoglycosides in 40. 7% of the isolates; and a single isolate positive for the blaDHA-16 gene. Despite the fact that antimicrobial resistance among Salmonella species from the dairy farms is low, our results contribute to the regional and national understanding of antimicrobial resistance in dairy herds in Australia.
Source link: https://doi.org/10.3168/jds.2021-21084
The AMR Scorecard provides laboratories with a scientific assessment device for improving the quality of bacterial culture, identification, and microbiological testing methods. Objective: To assess the results of the AMR Scorecard in 11 pilot laboratory assessments in three countries that were also rated with the Stepwise Laboratory Quality Improvement Process Towards Accreditation checklist, with the Stepwise Laboratory Quality Improvement Process Towards Accreditation checklist. The AMR Scorecard results were generally consistent with SLIPTA scores, which was also consistent with SLIPTA results. The AMR Scorecard identified scientific flaws in AMR experiments, and SLIPTA found flaws in the laboratories' quality control systems. Conclusion: Since the AMR Scorecard identified significant AMR testing that were not detected by SLIPTA, it is recommended that microbiology labs use SLIPTA and the AMR Scorecard in parallel when preparing for accreditation. Expanding the use of the AMR Scorecard is a priority to address the increasing use of quality clinical microbiology laboratory services in support of enhanced patient care and AMR monitoring.
Source link: https://doi.org/10.4102/ajlm.v11i1.1476
Salmonella enterica serovar 4,,12:i:-, a monophasic version of Salmonella Typhimurium lacking the phase 2 flagellin, is one of the most common serotypes responsible for Salmonellosis worldwide. This research was designed to determine antimicrobial resistance, the presence of antimicrobial resistance genes and virulence genes, and determine the MLST genotypes of Salmonella serovar 4, , 12:12:i:- isolates from Guizhou province, China, which includes isolates from Guizhou province, China. We found 363 non-typhoid Salmonella isolates of Guizhou from 2013 to 2018. The micro broth dilution technique revealed antimicrobial resistance genes and virulence genes by PCR, assaying molecular sequence typing by Multilocus sequence typing. MLST analysis revealed three sequence types, with ST34 being the most common sequence type. This report is the first review of Salmonella 4, ,12:i:- in humans from Guizhou province, China.
Source link: https://doi.org/10.1371/journal.pone.0266443
ABSTRACT Klebsiella pneumoniae is a pathogen that has been blamed for its high incidence of antimicrobial resistance. Here, we explore the role and mechanism of KbvR regulator in the reaction to environmental stress and the effects of osmotic stress on antimicrobial resistance. The expression level of ompK36 was reduced in response to high osmotic strain in the wild type, as well as the deletion of kbvR and outer membrane porin OmpK36. The binding of KbvR to the promoter of the ompK36 gene was confirmed by DNA affinity purification and microscale thermophoresis assay, indicating that KbvR has directly and positively regulated the expression of OmpK36. However, the increased ompK36 expression and the sensitivity to u03b2-lactams in the kbvR mutant strain of severe osmotic stress were lower than those of WT. In conclusion, our research has shown that elevated osmotic stress in the atmosphere contributed to the immune resistance of K. pneumoniae to antibiotics, as well as the fact that OmpR's oversight of OmpK36 was instrumental in combating high osmotic stress to change the antimicrobial resistance. To change the resistance of K. pneumoniae to u03b2-lactam antibiotics, this research shows that KbvR is a recently found regulator that can be upregulated under medical strain and specifically control the expression of OmpK36. The results show how adapting to high osmotic stress alters K. pneumoniae's sensitivity to antibiotics.
Source link: https://doi.org/10.1128/spectrum.00507-22
ABSTRACT Campylobacter is one of the most common bacterial pathogens responsible for campylobacteriosis in the United States. Poultry is recognized as a key source of Campylobacter transmission to humans. Campylobacter was not present in conventional supermarket and NAE retail chicken, and there were no differences between conventional supermarket and NAE retail chicken. However, none of these virulence genes were found in C. jejuni isolates from litter samples. Campylobacter prevalence in retail meat samples was higher than in lab-based samples obtained from farms, and there was no difference between Campylobacter prevalence in conventional and NAE retail chickens, according to our report. Importance The FDA antibiotic withdrawal policy has resulted in a change in the manufacturing process, from conventional antibiotic-fed birds to no antibiotics ever raised birds. On the farm or in retail chicken meats, the effect of this change to NAE on the prevalence and characteristics of Campylobacter has not been investigated.
Source link: https://doi.org/10.1128/spectrum.00251-22
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