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The identification of stabilizing amino acid alternatives in healthy proteins is a crucial difficulty in protein engineering. Advances in biotechnology have made it possible for appraising countless protein versions in a single high-throughput experiment, and more current studies use such data in healthy protein engineering. We have used GMMA to > 54,000 versions of environment-friendly fluorescent protein each lugging 1-15 amino acid substitutions. The six top-ranking substitutions are shown to progressively boost GFP and generally, our analysis recovers almost all the substitutions formerly reported to be advantageous for GFP folding and function, only making use of a single experiment as input.
Source link: https://doi.org/10.1101/2020.12.03.408732
Pattern matching is a crucial action in a selection of organic sequence analysis pipelines. The FM-index is a pressed information framework for pattern matching, with search run time that is independent of the length of the database text. AWFM-index is simple to incorporate into bioinformatics software and is able to do exact suit count and situate queries ~ 2-4x faster than SeqAn3's FM-index execution for nucleotide search, and ~ 2-6x faster for amino acid search in a single-threaded context.
Source link: https://doi.org/10.1101/2021.01.12.426474
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