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Allergen - Springer Nature

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Last Updated: 28 September 2022

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IgE and IgG4 Epitope Mapping of Food Allergens with a Peptide Microarray Immunoassay

Peptide microarrays are a quick and cost-effective method to identify linear epitopes of food allergens in a high-throughput fashion. The last one, however, has a special interest in food allergy research, as the development of tolerance to food allergens has been linked to a decrease in IgE and an increase in IgG4 levels against linear epitopes. Recent reports comparing the linear epitopes of food allergens with peptide microarray immunoassays have found peptide biomarkers that can be used to aid in the early detection of food allergies and diagnosis of tolerance impairments in early stages. This chapter discusses how microarray production is achieved by using a collection of overlapping peptides corresponding to the primary sequences of food allergens and data acquisition and analysis of IgE and IgG4 binding epitopes.

Source link: https://doi.org/10.1007/978-1-0716-2732-7_16


Prevalence and trend of allergen sensitization in patients with a diagnosis of stasis dermatitis referred for patch testing, North American contact dermatitis group data, 2001–2016

Introduction to the study of stasis dermatitis and allergic contact dermatitis was limited to few studies. Patients with a final SD diagnosis had similar percentages of u22651 positive allergic reaction but with a higher risk of allergic reactions to fragrance mix I, bacitracin, quaternium-15, Myroxylon pereirae, phenolic urea, diazolidinyl urea, and propylene glycol. ACD was also diagnosed with ACD, bolstering the importance of patch testing in select SD patients.

Source link: https://doi.org/10.1007/s00403-021-02295-y


Biochemical and clinical studies of putative allergens to assess what distinguishes them from other non-allergenic proteins in the same family

Several protein families have numerous members listed in databases as allergens; however, several allergen database entries, u201corphan allergens. u201d, are members of large families for which all other members are not allergens; however, some allergy-related database entries, u201d, are identified as allergens; however, some allergen database entries, herein, are members of large families, of which all other family members are not allergens. Examination of ChALDH and ChMDH crystal structures as well as a homology structure model of AaALDH revealed no definite epitopes that distinguish these putative orphan allergens from their non-allergenic protein relatives. Although published results showed significant human IgE reactivity with ChALDH, no SPT reactivity was observed in this study. With only one of these three orphanages, ChMDH, eliciting SPT responses that correspond to the protein's inclusion in allergy databases, this highlights the complex nature of bioinformatics' use to determine the potential allergenicity of food proteins that could be added to human diets and, where appropriate, the subsequent clinical testing of the bioinformatic analysis.

Source link: https://doi.org/10.1007/s11248-022-00316-8


Allergens from house dust and storage mites

House dust mites are one of the most common allergy triggers worldwide. While epidemics of the genus Dermatophagoides are widespread, Blomia tropicalis and storage mites are only of concern for particular areas or groups of people. Der p 1, Der p 2; Der p 2; and Der p 23; Der p 1, Der p 2, and Der p 23; with immunoglobulin E binding frequencies of more than 70% and high allergenic activity.

Source link: https://doi.org/10.1007/s40629-022-00226-5


Diagnosis of Apis dorsata venom allergy: use of recombinant allergens of Apis mellifera and a passive basophil activation test

A. dorsata venom anaphylaxis diagnosis: This research evaluated the use of four recombinant allergens of A. mellifera venom and the passive basophil activation test in the diagnosis of A. dorsata venom anaphylaxis. In patients with anaphylaxis to A. dorsata stings, patients were compared with serum IgE to four recombinant allergens of A. mellifera, rApi m 1, 2, 5 and 10 by Phadia ImmunoCAP, with serum IgE compared to the crude venom of A. mellifera or V. vulgaris by Phadia ImmunoCAP. Using a passive basophil activation test, Basophil activation in response to A. dorsata or V. affinis venom was assessed. Results rApi m 1 and 10 showed remarkably high positivity rate of 90% when combined, with A. mellifera's crude venom and a high optimismity rate of 90%. Whereas, IgE reactivity to rApi m 2 or 5 showed a strong link to V. vulgaris crude venom.

Source link: https://doi.org/10.1186/s12948-022-00178-9


Immortalised canine buccal epithelial cells’ CXCL8 secretion is affected by allergen extracts, Toll-like receptor ligands, IL-17A and calcitriol

Epithelial cells have been shown to produce mediators that can influence neighboring immune cells' behavior. Although the oral mucosa has piqued allergy relief and mucosal pathogen immunization in dogs, there are still no details regarding mediator secretion by canine oral epithelial cells. Canine buccal epithelial cells determined that the study's aim was to expand the understanding on the stimuli that may influence the secretion of certain pro- and anti-inflammatory cytokines as well as the chemokine CXCL8. Immortalized canine buccal epithelial cells stained positive for CD49f, but not for cytokeratin 3. Specifically studied, TLRL, IL-17A, and calcitriol modulate CXCL8 secretion in a cell line of canine buccal epithelial cells was found in this study.

Source link: https://doi.org/10.1186/s13567-022-01090-5


Poultry Meat allergy: a Review of Allergens and Clinical Phenotypes

More cases of poultry meat allergy have been documented in recent years, whether IgE- or non-IgE-mediated, according to the Purpose of analysis. Latest findings Poultry meat allergy is a result of cross-reactions with identified allergens of egg yolk or bird feathers, or as a result of true IgE-mediated sensitivity to allergens in poultry meat. Summary There is a wide variety of patients with poultry meat allergy, including clinical signs and cross-reactivity characteristics. Patients are particularly interested in knowing the various clinical aspects of reactions to poultry meat is a significant step toward accurate diagnosis and providing management options that are well accepted by patients.

Source link: https://doi.org/10.1007/s40521-022-00309-2

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions