Allergen - PubMed

Development of a simple and reliable LC-MS/MS method to simultaneously detect walnut and almond as specified in food allergen labelling regulations in processed foods.

Several varieties of walnut and almond, eight types of other nuts, and three standard allergen ingredients or cereals were chosen from a sample of walnut and almond 2S albumin and 7S globulin, as well as three target peptides from almond 11S globulin. When extracted walnut and almond protein were converted into butter cookie chocolate ice cream, the detection limit for the walnut 2S albumin peptide GEEMEEMVQSAR was 0. 22 bcg/g was exceeded by 0. 05 % u00b1 peptide GNLDFVQPPR was 0. 08 u00b1 0. 02 bcg/g.

Source link: https://doi.org/10.1016/j.crfs.2023.100444

Air pollution in the places of Betula pendula growth and development changes the physicochemical properties and the main allergen content of its pollen.

The purpose of the investigation was to determine the effect of air pollutants on the chemical composition of birch pollen and the Bet v1 protein's secondary structures. We have found modest variations in parameters describing the photosynthetic light reactions, similar spectra of leaf reflectance, and negligible differences in the discrimination values of the u03b413C carbon isotope. Mean Bet v1 concentration measured in pollen samples collected in Kraku00f3w was significantly higher than those obtained in less polluted locations, on the other hand, FT-Raman spectra showed the most significant differences in protein wavenumbers.

Source link: https://doi.org/10.1371/journal.pone.0279826

TNFR1 links TNF exocytosis to TNF production in allergen-activated RBL-2H3 cells.

We previously reported that maximum manufacturing of Tumor Necrosis Factor in antigen-activated RBL-2H3 cells requires Munc13-4, a regulator of exocytic fusion. In RBL-2H3 cells, we observed a strong correlation between the total TNF level and TNF exocytosis. These results, together, show that sTNF and TNFR1 function as an autocrine agent and receptor respectively on the mast cell surface to promote TNF production in allergic inflammation.

Source link: https://doi.org/10.1016/j.cellsig.2023.110607

Precautionary allergen labelling: What advice is available for health care professionals, allergists and allergic consumers?

Prioritizing food allergens during processing was first introduced as a judicious risk management tactic to address instances of u00ab unavoidable u00bb cross-contact with priority food allergens during food processing. Most food items currently have no discernable allergen residue or contain only small amounts of residue of the allergens identified using PAL; however, occasional high concentrations of allergen residue are reported, making it an ineffective risk communication tool for allergic consumers. Healthcare professionals are increasingly recommending to patients who are suspected of being 'not allergic' to foods displaying a PAL label, owing to the increased use of PAL on prepacked food items and in order to maximize diet choices for allergic people. This article explains why eating PAL-containing products is not advisable without undergoing a complete medical analysis and knowing that an allergen risk assessment has been carried out.

Source link: https://doi.org/10.1016/j.jaip.2022.12.042

Allergen immunotherapy for respiratory allergies in clinical practice: A comprehensive review.

Allergen-specific immunotherapy is the only therapy that alters the underlying pathophysiology of IgE-mediated allergic diseases. Evidence shows the effectiveness of achieving better control of the signs and reduction of medication use in patients with allergic rhinoconjunctivitis and/or asthma. In addition, it may also prevent the propagation and propagation of disease ranging from allergic rhinitis to asthma in children. In addition, there is urgent need for real-world reports in developing countries regarding AIT's cost-effectiveness analysis and optimization of AIT schedules and products, ensuring that medical research and implementation of AIT for respiratory allergic diseases can be efficient and safe.

Source link: https://doi.org/10.12932/AP-260722-1418

The Effectiveness of Allergen Immunotherapy in Adult Patients with Atopic Dermatitis Allergic to House Dust Mites.

Allergen immunotherapy is not a first-line therapy for atopic dermatitis, but its effectiveness has been criticized. Objects: The efficacy and safety of AIT in adult patients with AD and monosensitization to house dust mites were investigated. Patients were eligible if they were diagnosed with AD; had moderate-to-severe AD; had moderate to severe AD; and, remarkably, positive for the specific immunoglobulin E response to D. pteronyssinus and D. farinae extracts; as well as Der p1 and Der f1. For 12 months, the patients were given Purethal mites containing the extract allergens D. pteronyssinus and D. farinae, or a placebo. HDM-AIT's results: HDM-AIT's findings: HDM-AIT improved atopic dermatitis in patients who were strictly suited for desensitization with a documented monovalent mite allergy.

Source link: https://doi.org/10.3390/medicina59010015

Component-Resolved Diagnosis Based on a Recombinant Variant of Mus m 1 Lipocalin Allergen.

We compared the sequence of the variant Mus m 1. 0102 to other Mus m 1 allergen members and developed Discotope 2. 0 to predict conformational epitopes based on its 3D-structure, considering the importance of sequence/structure analysis in protein antigenic reactivity. The antigen source for mouse allergy is based on serum IgE testing, which uses an epithelial extract as the antigen source. Recombinant Mus m 1. 0102, a key allergen responsible for mouse allergy, was a useful device in investigating fine epitope mapping of specific IgE reactivity to the common allergen responsible for mouse allergy.

Source link: https://doi.org/10.3390/ijms24021193

Ragweed Major Allergen Amb a 11 Recombinant Production and Clinical Implications.

Ragweed pollen is highly allergenic and causes type I hypersensitivity reactions in the affected populations. Amb a 11 was produced in parallel with patient recruitment. In E. coli and Sf9 Spodoptera frugiperda cells, the mature protein gene was introduced. In 150 patients' sera for both recombinant allergen forms in ELISA, and 5 positive sera were tested further by hRBL hexosaminidase issay, with 5 positive sera being tested further by hRBL hexosaminidase release assay. Compared to nAmb's 1. 01, a significant, marginally smaller effect of recombinant allergens was found in the hRBL mediator's release assay. In conclusion, recombinant Amb a 11 is a bona fide allergen and a source of hRBL degranulation. It is a common IgE-reactive component of ragweed pollen, with high IgE sensitization prevalence in the sample population and allergy sensitivity of the recombinant allergen comparable to Amb a 1.

Source link: https://doi.org/10.3390/biom13010182

Binding to Iron Quercetin Complexes Increases the Antioxidant Capacity of the Major Birch Pollen Allergen Bet v 1 and Reduces Its Allergenicity.

Bet vs. 1 is the most common allergen in birch pollen, with up to 95% of patients sensitivity to birch pollen. We reviewed Bet vs. sequester iron and its immunomodulatory properties on human immune cells as a result. FeQ2 was determined in docking estimates and by spectroscopy. With the human reporter cell line AZ-AHR, human mast cells and Arylhydrocarbon receptor activation were investigated on human mast cells and Arylhydrocarbon receptor activation. With calculated Kd values of 1 nm surpassing affinities to quercetin alone nearly by a factor of 1000, Bet vs. 1 bound to FeQ2 is a strong determinant of 1000. Sentized human mast cells were confined to FeQ2-masked IgE epitopes and reduced IgE binding up to 80% and impaired degranulation of sensitized human mast cells. Nourishing immune cells with complex iron may also be a promising antigen-independence immunotherapeutic strategy to improve allergy immunotherapy's effectiveness.

Source link: https://doi.org/10.3390/antiox12010042

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