Allergen - PubMed

The Humanised NPY-mRFP RBL Reporter Cell Line Is a Fast and Inexpensive Tool for Detection of Allergen-Specific IgE in Human Sera.

For decades as a model of high-affinity Immunoglobulin E receptor signaling in Rat basophilic leukaemia cells, as a model of high-affinity Immunoglobulin E receptor signalling. In huNPY-mRFP cells, the equivalent to the 500,000 Fc-Rab1 cells per cell in the RS-ATL8 humanised luciferase reporter, a human Fc0RIu03b1* reporter, which is a human Fc3b5RI U03b1 gene for transfection, resulting in the deletion of known endoplasmic reticulum retention signals and high surface expression of human Fcu03b1­u03b3RIu03b3RI u03b3RIu03b3RIu03b3RIu03b5RIu03b3RI-u03b3RIu03b5RIu03b5RIu03b3RIu03b5RIu03b1-u03b5RIu03b3RIu03b1B3B1RIu03b1u03b3b1u03b1u03b3B5RIu03b1u03b3B5RI.

Source link: https://doi.org/10.3390/diagnostics12092063

Red emitting fluorogenic dye as an efficient turn-on probe for milk allergen.

A detailed spectroscopic study shows that QR binding of QR to the hydrphobic calyx site of u03b-LG protein greatly reduces the torsional agility and propensity of TICT state formation of QR, making the dye highly fluorescent in nature. The estimated u03b2-LG concentrations in milk and whey matrices from fluorometric titration and densitometry methods were found to match excellently with each other, showing the possibility of QR as an effective turn-on fluorescent probe for the quantification of u03b2-LG in several dairy products.

Source link: https://doi.org/10.1016/j.ijbiomac.2022.09.130

Allergen-specific immunotherapy induces monocyte-derived dendritic cells but attenuates their maturation and cytokine production in the lesional skin of an atopic dermatitis mouse model.

Atopic dermatitis is a common inflammatory skin disease, but current therapies for AD are mostly limited to symptoms relief and inflammation reduction. We performed ASIT on this AD mouse model by dinitrofluorobenzene and an extract of Dermatophagoides farina, to determine ASIT's effectiveness and investigate its underlying mechanisms. As immature and semi-mature dendritic cells preferentially promote tolerance, accumulation, and proliferation, accumulation has accelerated, accumulation, development of monocyte-derived dendritic cells following ASIT may indicate a new mechanism of ASIT as a prospective therapeutic target for AD.

Source link: https://doi.org/10.1111/1346-8138.16582

Identification of allergen encoding sequences in a novel food ingredient from Moringa oleifera leaves.

A protein source with a balanced amino acid content is required to feed the world's growing population, such as Moringa oleifera leaves, a protein source with a balanced amino acid composition. Since Moringa leaf proteins is a new food in the EU and the United Kingdom, an analysis of their potential allergenicity is required. Using traditional proteomic techniques, proteins extracted from Moringa leaf powder were characterized. Moritides were discovered as potential allergens by homology with a latex allergen implicated in fruit-latex syndrome by in-silico analysis.

Source link: https://doi.org/10.1016/j.foodchem.2022.134185

Targeting an Initiator Allergen Provides Durable and Expansive Protection against House Dust Mite Allergy.

Whereas treatment of allergic diseases such as asthma depends largely on dysregulated effector pathways, the most feasible alternative to stopping them by a pharmaceutical, at-source involvement has been stymied until now by ambiguities regarding optimal targets and drug development challenges. House dust mites are the most common allergens of allergy worldwide. Their degradome has a strong disease correlation that supports their status as risk and initiator allergens that act immediately and indirectly through bystander effects on other allergens. Our aim was to see if target-selective inhibitors of group 1 HDM allergens could be a safe route to novel therapies. We undertook the first investigation into whether pharmaceutically developed inhibitors of group 1 allergens could provide protection against HDM exposure. When presented with an HDM isolate containing the HDM allergome, the compounds suppressed acute airway responses of rats and mice.

Source link: https://doi.org/10.1021/acsptsci.2c00022

Immortalised canine buccal epithelial cells' CXCL8 secretion is affected by allergen extracts, Toll-like receptor ligands, IL-17A and calcitriol.

Epithelial cells are known to produce mediators that can influence neighboring immune cells' behavior. Although the oral mucosa has piqued allergy onset and mucosal pathogen immunization in dogs, there is only limited information regarding mediator secretion by canine oral epithelial cells. Canine buccal epithelial cells were found to expand the understanding of the stimuli that can influence the secretion of some pro- and anti-inflammatory cytokines, as well as the chemokine CXCL8. Buccal epithelial cells were isolated from a dog's biopsy and immortalized by lentiviral transduction of the SV40 large T antigen. Immortalized canine buccal epithelial cells stained positive for CD49f but not for cytokeratin 3. In a cell line of canine buccal epithelial cells, such allergens, TLRL, IL-17A, and calcitriol modulate CXCL8 secretion were discovered in this research.

Source link: https://doi.org/10.1186/s13567-022-01090-5

Health and economic impact of subcutaneous allergen immunotherapy in patients with pollen-induced allergic rhinoconjunctivitis: real-word evidence from the Czech Republic.

Background: The prevalence of allergic rhinoconjunctivitis has gradually grown over the years, but allergen immunotherapy remains the only disease-modifying therapy. The cost of symptomatic medication decreased by 49%, while unscheduled specialist visits decreased by 73%. Except for AIT's AIT administration, total healthcare expenses dropped by 54% compared to the baseline pollen season without AIT. Conclusion: Subcutaneous AIT is a safe alternative in clinical practice, saving on symptomatic drugs and unscheduled consultations.

Source link: https://doi.org/10.2217/imt-2022-0143

Comparison of protein extraction protocols and allergen mapping from black soldier fly Hermetia illucens.

LC-MS/MS-based proteomics experiments were used herein to determine the protein extraction efficiencies for a variety of extraction buffers and the effects of ingredient processing on proteome and allergen detection. The highest number of protein groups from three different types of BSF samples was found by SDS-based buffers, according to Discovery proteomics. 33 putative allergens were identified after using multi-level filtering techniques, comparing the detected BSF proteins to sequences from public allergen protein databases. Using peptide abundance measurements, a targeted LC-MS assay was created for the pan-allergen tropomyosin and used to determine the effect of buffer composition and ingredient processing. Processed and unprocessed black soldier fly samples can be tested by fingerprinting, protein extraction buffer, and the processing technique, according to our results. In total, 33 putative allergens were identified by comparing the detected BSF proteins to sequences from public allergen protein databases.

Source link: https://doi.org/10.1016/j.jprot.2022.104724

Allergen recognition by specific effector Th2 cells enables IL-2-dependent activation of regulatory T-cell responses in humans.

We found that continuing food allergen exposure in the form of oral immunotherapy resulted in an elevated risk of Tregs in people with persistent clinical tolerance. We wanted to identify regulatory components of the CD4+ T-cell response to food allergens by testing their functional activation in vitro and in vivo. Following the initiation of the allergy-specific Th2 response, In vivo exposure to peanut in the form of an oral food challenge of allergic subjects resulted in a delayed and persistent activation of Tregs. The novel finding of our study shows that a continuous wave of Treg activation is triggered by the release of IL-2 from Th2 effector cells, resulting in the suggestion that therapeutic administration of IL-2 may improve current OIT approaches.

Source link: https://doi.org/10.1111/all.15512

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