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Allergic asthma has been attributed to dysfunctional bronchial epithelial secretion of interferons, which may be causally linked to an elevated risk of viral exacerbations. We've previously reported that allergen immunotherapy helps asthma outbreaks and prevents respiratory infections that require antibiotics; however, whether AIT alters antiviral immunity is still unclear. To investigate the effects of house dust mite-sublingual allergen immunotherapy on the bronchial epithelial antiviral and inflammatory responses in patients with allergic asthma. Adult patients with HDM allergic asthma who were exposed to HDM allergy asthma received HDM-SLIT 12-SQ or placebo for 24-weeks in this double blind controlled trial. Both at gene and protein level, increased poly-induced expression of IFN-u03b2 in HDM-SLIT. IL-33's IL-33 gene expression was also elevated, while IL-33 was lessened. IL-6 and TNF-u03b1 in the HDM-SLIT group, on the other hand, increased in pro-inflammatory cytokines IL-6 and TNF-u03b1. These findings may help to determine the effectiveness of HDM-SLIT in reducing allergic asthma exacerbations.
Source link: https://europepmc.org/article/MED/36701676
The aim of the investigation was to determine the effect of air pollutants on the chemical composition of birch pollen and the Bet v1 protein's secondary structures. We have noticed slight variations in the parameters describing the photosynthetic light reactions, identical spectra of leaf reflectance, and negligible differences in the discrimination values of the u03b413C carbon isotope are found. On the physiological condition B. pendula specimen, the obtained results reveal no evidence of a trace of pollution. Mean Bet v1 concentration in pollen samples collected in Kraku00f3w was significantly higher than that in less polluted locations, on the other hand, while FT-Raman spectra showed the most significant variations in protein wavenumbers.
Source link: https://europepmc.org/article/MED/36696393
During allergen bronchoprovocation testing, house dust mite produces more responses than other allergens. The maximum reduction in forced expiratory volume in a second is reported by ABP's maximum fall in a second and the region under the FEV 1 vs time curve. We wanted to determine if the EAR recovery period for HDM would be longer than other allergens, as well as the early asthmatic response and the late asthmatic response. We compared EAR and LAR FEV 1, max, AUC, and absolute difference in FEV 1, max to the most recovered FEV 1 in FEV 1, max to the most recovered FEV 1. Max of the EAR AUC and FEV 1, max varied between allergens, but the LAR AUC and FEV 1, max did not differ, but not a lot.
Source link: https://europepmc.org/article/MED/36681834
We previously reported that the maximum production of Tumor Necrosis Factor in antigen-activated RBL-2H3 cells requires Munc13-4, a regulator of exocytic fusion. TNF production is based on a combination of several fusion catalysts investigated in this report. In RBL-2H3 cells, there was a strong correlation between the total TNF level and TNF exocytosis. These results indicate that sTNF and TNFR1 act as both an autocrine agent and receptor respectively on the mast cell surface to promote TNF production during allergic inflammation.
Source link: https://europepmc.org/article/MED/36690134
Most food products currently do not have detectable allergen residue or contain only low amounts of residue of the allergens specified using PAL; however, occasional high levels of allergen residue are found, making it an ineffective risk communication tool for allergic consumers. Several reasons exist, making the consumption of PAL statements that are not recommended for people with food allergies difficult. Because of the increased use of PAL on prepackaged food items, and in order to improve diet choices for allergic patients, healthcare professionals have continued to recommend that some patients who are not able to eat products marked with a PAL logo. This article explains why taking PAL-based products is not advisable without having a complete medical analysis and knowing that an allergen risk assessment has been conducted.
Source link: https://europepmc.org/article/MED/36682535
Background The immunological benefits of allergen-specific immunotherapy are well documented, but no studies have looked at the long-term effects of pollen subcutaneous immunotherapy on children and adolescents' health-related quality of life. In a three-year follow-up, the aim of this research was to determine the effects of pollen SCIT on HRQoL and determining the relationship between HRQoL and allergic rhinoconjunctivitis in children and adolescents with allergic rhinoconjunctivitis. The association of HRQoL and influencing factors was investigated over time, between groups and a linear mixed model for the association between HRQoL and influence factors. After a year of SCIT, the percentage of children with severe or severe symptoms decreased from 35. 6% to 4. 5 percent. During the investigation period, IgE antibodies decreased from 151. 0 to 76. 8 kU/L birch, and IgG4 antibodies increased, birch from 151. 0 to 17. 6 g/L, grass from 0. 5 to 17. 3 g/L, with a 0. 2 g/L increase.
Source link: https://europepmc.org/article/MED/36650597
In IgE-mediated food allergies, exposure to the allergen stimulates systemic allergic reactions. Oral immunotherapy treats food allergies by incremental increases in oral allergen exposure. We therefore examined allergen-specific antibodies from two OIT cohorts of patients with persistent and transient responses. We compared antibodies from people with persistent or transient responses to identify specific tolerance-associated epitopes of the immunodominant allergen Ara h 2 identified by neutralizing antibodies. Our findings show the molecular basis of antibody-mediated food allergy by showing how these antibodies interfere with IgE-allergen interactions to prevent allergic reactions. Our approach to discovering antibodies' structural and functional bases helps illustrate the clinical relevance of specific antibody clones in antibody-mediated tolerance. Our findings will help with treatments of peanut allergy using neutralizing antibodies and hypoallergens. We hope that our findings will form the basis for peanut allergy therapies.
Source link: https://europepmc.org/article/MED/36647835
Ragweed pollen is highly allergenic and causes type I hypersensitivity reactions in the affected populations. In parallel with patient recruitment, the production of recombinant Amb a 11 was carried out. In E. coli and Sf9 Spodoptera frugiperda cells, the mature protein gene was inserted. Both recombinant allergen forms in ELISA were tested in 150 patients' sera for both recombinant allergen forms in ELISA, according to hRBL hexosaminidase release assay, with five positive sera being tested further. When compared to nAmb a 1. In conclusion, recombinant Amb a 11 is a bona fide allergen and an inducer of hRBL degranulation. It is a common IgE-reactive component from ragweed pollen, with high IgE sensitization prevalence in the sample population and allergen similar to Amb a 1.
Source link: https://europepmc.org/article/MED/36671567
Background IgE-blocking antibodies are a hallmark of allergen immunotherapy. Methods Serum samples from 24 patients were collected before and regularly during AIT's birch pollen testing. According to ELISA and ImmunoCAP, respectively, Bet vs. 1-specific IgG1 and IgG4 levels were determined by ELISA and ImmunoCAP. The inhibition of Bet vs. 1-induced basophil activation was measured in unmodified and IgG1- or IgG4-depleted samples. Bet vs. 1-antibody complexes was compared by ELISA and surface plasmon resonance to determine their stability. AIT levels in 12 and 24 months peaked at 10 and 24 months, respectively. In the first year of therapy depletion of IgG1, the inhibition of basophil formation was considerably reduced, although the absence of IgG4 almost prevented IgG1 blocking. Bet vs. 1-IgG1 complexes were more stable than Bet vs. 1-IgG4 complexes, which were far more reliable than Bet vs. 1-IgG4 complexes.
Source link: https://europepmc.org/article/MED/36657603
History Peanut allergy is regarded as a significant food allergy that can cause acute and even fatal symptoms. Cold plasma is now considered a non-thermal means to alter protein structure and has the ability to decrease protein immunoreactivity in protein allergens. The application of a cold argon plasma jet to peanut protein extract could reduce the amount of a 64 kDa protein band corresponding to a common peanut allergen Ara h 1 using SDS-PAGE analysis, but the overall protein distribution did not change significantly. After five scans of cold argon plasma jet therapy, the reduction was down to 66% after only 15 minutes of therapy, and after about 15 minutes of treatment, the antigenicity was reduced by 38 percent. Conclusions The results indicate that cold argon plasma jet therapy may be a safe device for reducing peanut protein immunoreactivity.
Source link: https://europepmc.org/article/MED/36646652
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