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Allergen - Europe PMC

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Last Updated: 28 September 2022

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Quantum-dot-based sandwich lateral flow immunoassay for the rapid detection of shrimp major allergen tropomyosin

Tropomyosin has long been recognized as the most common allergen in shrimp, causing both food safety and public health alarm around the world. TM detection in processed foods was demonstrated by a simple, fast, specific, and precise quantum-dot-based lateral flow Immunoassay in the present study. respectively, 0. 01 mm U03bcg mLs and 0. 05 bb9 were reported as the visual detection threshold and the instrumental detection threshold of were 0. 05 u00b9 and 0. 01 mL, respectively. In addition, LFIA's ability made 8 of nine real food samples TM-positive by the validated ELISA technique. In addition, 0. 45 bcg gu202bu00b9 of model processed foods caused TM in model processed foods, which could also be detected by the developed assay. These results showed that the LFIA developed in this study had a major impact on the rapid and accurate detection of allergens in shrimp foodstuffs, related products, and their production lines.

Source link: https://europepmc.org/article/MED/IND607838069


Proteolytic activity accelerates the T H 17/T H 22 recall response to an epicutaneous protein allergen-induced T H 2 response.

Both original skin sensitization and distant skin sites have been inducible by re-exposure to an allergen. Sensitization with papain or HDM, which have protease activity, resulted in the distinction between T H 17 and T H 2, as well as T H 2. T H 2 and T H 22 cytokines were released in papain- or HDM-sensitized mice, a new single challenge to the ear skin triggered the expression of T H 2 and T H 22 cytokines. These results show that allergenic proteins result in the differentiation of T H2 in skin DLN cells and an antibody response. In addition, allergen proteins that contain protease activity can also distinguish T H 17 and produce T H 2 and T H 22 recall responses at epicutaneous challenge sites.

Source link: https://europepmc.org/article/MED/35378053


Genome assembly and annotation of Periplaneta americana reveal a comprehensive cockroach allergen profile.

Background The American cockroach is one of the most common cockroach species in urban areas, and has been shown to have an elevated risk of allergies and asthma. Methods P. americana, genetic, transcriptomic, and proteomic methods were used to improve allergen information. In Thai patients allergic to P. americana, then tested allergens using enzyme-linked immunosorbent assay. Results The chromosomal-level genome of P. americana has been assembled with 94 percent completeness at BUSCO, and its contiguity has been greatly enhanced. Using bioinformatics and proteomic technologies, a new isoallergen of tropomyosin and several potential isoallergens of Per a 5 were discovered. In addition, a comparative analysis of P. americana with the closely related Blattodea species revealed the possibility of cross-reaction. P. americana's high-quality genome and proteome analysis of cockroach allergens can be useful in determining cockroach allergens at the molecular level. In Per a 7, seven novel allergen groups and one isoallergen were identified, as well as one isoallergist.

Source link: https://europepmc.org/article/MED/36153808


Egy régi-új allergén - a propolisz szenzibilizáló hatásának vizsgálata 1992 és 2021 között.

Objective The aim of this research was to examine the 30-year records of patients with propolis hypersensitivity. In the Allergology Outpatient Unit of the Department of Dermatology, Venereology, and Dermatooncology of the Semmelweis University, patch tested patients between 1992 and 2021. Propolis sensibility had piqued 464 patients. According to propolis sensibility, we present an annual analysis of the frequency of propolis sensibility, including the most common diagnoses, age groups, localizations most affected by clinical signs, and co-hypersensitivities. Conclusions In the 30-year course, the sensitization frequency of propolis was on average of 2. 6%; in 2019-2021, we detected a sudden rise. Hands, legs, and the face were all evidently affected by the skin disease. Contact hypersensitivity has risen since propolis products are used mostly on inflamed skin with higher allergen penetration. Propolis sensibility, as well as potential cross- and cohypersensitivities may lead to contact dermatitis or the occurrence of specific skin diseases.

Source link: https://europepmc.org/article/MED/36153726


RNA sequencing of single allergen-specific memory B cells after grass pollen immunotherapy: two unique cell fates and CD29 as a biomarker for treatment effect.

Background: Sublingual immunotherapy for grass pollen allergy can alter allergic rhinitis' natural history and is associated with elevated allergen-specific IgG 4 levels. We investigated changes in allergen-specific BMEm subsets induced by SLIT for grass pollen allergy to learn more about the crucial role memory B cell responses play in mediating SLIT's beneficial effects. Methods Blood samples were obtained twice outside of the pollen season, from twenty-seven patients with sensitization to ryegrass pollen and seasonal rhinoconjunctivitis. Four months of SLIT increased RGP-specific IgE and IgG 4 in serum and established two Lol p 1-specific Bmem subsets with unique transcriptional profiles. Since SLIT, there was an increase in the percentage of Lol p 1+ Bmem expressed surface IgG 4, CD23, and CD29. Bmem fates are two transcriptionally unique BMEm fates that resulted from a four-month course of SLIT for grass pollen allergy.

Source link: https://europepmc.org/article/MED/36153670


Allergen immunotherapy, cancer, and immune disorders.

Purpose of the report The aim of this article was to give update on the enigtive relationships between allergies, allergen immunotherapy, autism, and immune disorders. Allergic diseases and cancer are on the rise and prevalence of these diseases, and a potential connection, or not, has been proposed for many years. Latest findings According to the text, recent research indicates that there could be some causative links between certain types of cancer and allergic diseases. Certain forms of cancer may be more linked to the presence of an allergic disease than others. Summary The connection between allergy and cancer is both complex and depends on the particular allergy and the specific organ under consideration. All forms of allergies regarding pancreatic cancer, colorectal cancer, and glioma have been shown to be a protective factor. Conversely, asthma is a risk factor for lung cancer, as well as atopic dermatitis for skin cancer.

Source link: https://europepmc.org/article/MED/36165426


Allergen bronchoprovocation: Correlation between FEV1 maximal percent fall and area under the FEV1 curve and impact of allergen on recovery.

During allergen bronchoprovocation testing, house dust mite produces more responses than other allergens. During the early asthmatic response and the late asthmatic response, Aims Our first aim was to determine the correlation between FEV 1, max and AUC. Methods We retrospectively estimated EAR and LAR AUC for each identified allergen for each listed allergen. We compared EAR and LAR FEV 1, max, AUC, and absolute difference in FEV 1, max to the most recovered FEV 1 3 hours post-challenge for each allergen. Max did not differ between the listed allergens on the chart, but the LAR AUC and FEV 1, max did not differ, but the FEV 1, max did not differ.

Source link: https://europepmc.org/article/PPR/PPR547904


Allergen-specific immunotherapy induces monocyte-derived dendritic cells but attenuates their maturation and cytokine production in the lesional skin of an atopic dermatitis mouse model.

ASIT performed on this AD mouse model by dinitrofluorobenzene and an extract from Dermatophagoides farina, we performed ASIT on this AD mouse model by subcutaneous injection of Dermatophagoides farina extracts to assess ASIT's effectiveness and investigate the causal mechanisms. ASIT's findings showed that it did not only reduce AD mouse skin lesions and scratching habits but also decreased their Th2-type immune responses. As immature and semi-mature dendritic cells preferentially promote tolerance and production, accumulation is less likely to persist, but monocyte-derived dendritic cells' maturation after ASIT may indicate a novel mechanism of ASIT and a potential therapeutic target for AD.

Source link: https://europepmc.org/article/MED/36117445


Red emitting fluorogenic dye as an efficient turn-on probe for milk allergen.

According to a detail spectroscopic analysis, QR-binding of QR to the hydrophobic calyx site of u03b2-LG protein greatly reduces the torsional mobility and propensity of TICT state formation of QR, making the dye highly fluorescent in nature. The estimated u03b2-LG determinations in milk and whey matrices from fluorometric titration and densitometry techniques are found to match well with each other, suggesting that QR could be a fast turn-on fluorescent probe for the quantification of u03b2-LG in many dairy products.

Source link: https://europepmc.org/article/MED/36122782


Fluorescence detection of milk allergen β-lactoglobulin based on aptamers and WS 2 nanosheets.

In this paper, a fluorescence assay based on WS 2 nanosheets and a fluorescent dye-labeled u03b2-Lg aptamer was designed to detect u03b2-Lg rapidly with high sensitivity and sensitivity. The u03b2-Lg aptamer is adsorbed on the WS 2 nanosheet surface by van der Waals' van der Waals forces, which cause the phenomenon of fluorescence resonance energy exchange and reduce the fluorescence signal in the system. When u03b2-Lg is on display, the conformation of the aptamer is specifically tailored to u03b2-Lg changes. The aptamer is subsequently isolated from the WS 2 nanosheet surface, and the fluorescence signal is recovered.

Source link: https://europepmc.org/article/MED/35403657

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions