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Dermatophagoides ssinus, Dermatophagoides farinae, and their allergens are among the most common household dust mites. Blomia tropicalis is also extremely common. The Brazilian Association of Allergy and Clinical Immunology Taskforce on AIT has produced this position paper on respiratory allergy and Hymenoptera venom allergy in respiratory allergy and Hymenoptera venom allergy. AIT in Brazil: This position paper aims to provide the main recommendations for AIT in Brazil, according to the latest scientific literature, which has been adapted to Brazilian context. The various studies that established the clinical settings for AIT recommended by the leading Allergy, Asthma, and Immunology world companies were reviewed and adaptable to the Brazilian situation. Conclusion: This position paper introduces the main recommendations for AIT's safe clinical practice in Brazil, utilizing recent findings of evidence-based medicine and precision medicine.
Source link: https://doi.org/10.1016/j.waojou.2022.100697
For decades, Rat basophilic leukaemia cells have been used as a model of high-affinity Immunoglobulin E receptor signalling. By EgEF-1, engulosus elongation factor 10. 5 B2 and, to a lesser extent, EgEF-1u03b2 and, by a factor similar to EgEF-immunoreactive EgEF-1u03b2/u03b4 (which had previously identified as IgE-immunoreactive EgEF-1u03b2 and a lesser extent by EgEF-1u03b2 eti.
Source link: https://doi.org/10.3390/diagnostics12092063
To date, there are reports on the development of delivery systems based on glycyrrhizic acid and its derivatives, but no such a delivery method has been used for allergen-specific therapy until now. It is also known that GA has an anti-inflammatory function, shifts the balance toward Th1, and raises the number of Treg cells, increasing the number of Treg cells, raising the risk of unwanted side effects. Similarly, the study of the immunomodulatory effects of GA's supramolecular complexes with allergen extracts is encouraging for the development of new drugs for AIT.
Source link: https://doi.org/10.3390/nano12010148
Canine atopic dermatitis is a chronic and often complicated by Staphylococcal infections. In a case of persistent CAD, here we discuss the consequences of allergen exposures on infection formation. Diagnosis of pyoderma was based on clinical findings and consistent cytology. Study 1 looked at the effect of duration of exposure while keeping the daily dose steady. The study 2 looked at new daily doses while keeping the total weekly dose and duration unchanged, while keeping constant the total weekly dose and duration unchanged. The longer the exposure, the more dogs were able to develop pyoderma. In the study 2, low-dose daily exposure caused more infections than high-dose infrequent exposure.
Source link: https://doi.org/10.3390/vetsci9010008
Mal d 1 is the most common apple allergen in northern Europe, and it is the first apple allergen. According to produce sufficient quantities, it is necessary to have an optimized production route and unsophisticated purification step for Mal d 1 and related mutants. We discuss a process for the transformation of the plasmid in viable E. coli cells, protein expression, and rapid one-step purification. Immobilized metal ion affinity chromatography and fast-protein liquid chromatography using a high-resolution anion exchange column, respectively, purified r-Mal d 1 with and without a polyhistidine-tag. For both mutants, an adequate yield of r-Mal d 1 with purity higher than 85% is obtained. The allergen is identified after tryptic gel digestion by peptide analyses using HPLC-MS/MS. In addition, the effect of the freeze and thawing process, freeze drying, and storage on dimer formation has been investigated.
Source link: https://doi.org/10.3390/mps4010003
Shellfish allergy affects 2% of the world's population and persists for life in the majority of patients. Due to the similarity of allergenic proteins from other invertebrates, diagnosis of shellfish allergy, especially shrimp, is difficult. Despite the clinical significance of immunological cross-reactivity among shellfish species and among allergenic invertebrates such as dust mites, the underlying molecular basis is not well understood. Here we mined the complete transcriptome of five commonly consumed shrimp species to identify and compare allergens with all known allergy sources. Using two major allergen databases, WHO/IUIS Allergen Nomenclature and AllergenOnline, all seven identified crustacean allergens were successfully identified, confirming all seven recognized crustacean allergens. This first transcriptomic review of allergens in a large food source is a useful tool for investigating shellfish allergens, comparing invertebrate allergens, and future development of improved diagnostics for food allergy.
Source link: https://doi.org/10.3390/ijms22010032
Metabolic reprogramming increases the ability of the innate immune cells to respond to a secondary stimulus, which is a critical step in the induction of trained immunity. Criteria: We hypothesize that specific metabolic reprogramming of lung trained macrophages induced by respiratory virus disease in allergic asthma sufferers. MethodsNeonatal mice were infected and sensitized by the Pneumonia virus of mice, a mouse equivalent strain of human RSV, mixed with ovalbumin. Adoptive transfer, metabolic inhibitor, and recovery experiments were all used to determine the role of specific metabolic reprogramming in childhood allergic asthma in children. Following the OVA challenge in childhood of mice, PVM infection, metabolic enzyme dysfunction, and OVA sensitization in neonatal mice resulted in non-Th2 type allergic asthma. Proline metabolism may be a good start in preventing allergic asthma in childhood.
Source link: https://doi.org/10.3389/fimmu.2022.977235
Abstract Epithelial cells have been found to produce mediators that can influence neighboring immune cells' behavior. Although the oral mucosa has piqued allergy desensitization and mucosal pathogen immunization in dogs, there is still no information about mediator secretion by canine oral epithelial cells. Canine buccal epithelial cells grew to expand the understanding on the stimuli that can influence the behavior of some pro- and anti-inflammatory cytokines and the chemokine CXCL8. Buccal epithelial cells were isolated from a dog's biopsy and immortalized by lentiviral transduction of the SV40 high T antigen. CD49f positiv, but not for cytokeratin 3. According to this report, canine buccal epithelial cells, such allergens, TLRL, IL-17A, and calcitriol modulate CXCL8 secretion are present in a cell line of canine buccal epithelial cells.
Source link: https://doi.org/10.1186/s13567-022-01090-5
MicroRNAs have gained scientific interest for their role in allergic disease pathophysiology as well as potential as biomarkers in allergen-specific therapy options. MiRs can alter dysregulated expression of certain bodily functions and contribute to or cause of certain diseases, but in some cases prevent or repress the development of various diseases. We review recent study on the role of specific miRs in controlling immune responses in epithelial cells and specialized immune cells in response to several stimuli, including allergic disorders, and limitation in allergy-specific immunotherapy.
Source link: https://doi.org/10.3389/falgy.2022.993937
Around the world, canned tuna is one of the most popular and most commonly consumed items in the seafood industry. Fish allergens have been identified as the biggest issue regarding food safety in these seafood products and are listed as the top eight food allergies in recent years, but fish allergens have been uncovered in recent decades. Parvalbumin is the most common of all allergens in the group of fish allergens. Before the parvalbumin-specific encoded gene amplification, the effects of pH, calcium content, and DNA quality of canned tuna was also investigated. The discovery of parvalbumin in commercially canned tuna was triggered by can-filling mediums, thermal conductivity, calcium content, and the acidity of various ingredients in food matrices, according to the obtained results. Allegens encoding gene analyses by agarose electrophoresis, according to this review, could be used as a reliable method for other food-borne allergens in complex food matrices.
Source link: https://doi.org/10.3390/molecules27175674
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