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Allergen - DOAJ

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Last Updated: 28 January 2023

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RAGE contributes to allergen driven severe neutrophilic airway inflammation via NLRP3 inflammasome activation in mice

Although there has been significant strides in the treatment of asthma, subsets of patients with airway neutrophilia are often more symptomatic and are unlikely to respond to traditional corticosteroid therapy. Pharmacological inhibition of NLRP3 dampened the effects of AA exposure and NLRP3 inflammasome activation was RAGE-dependent. In the BALF, unusual population of SiglecF+ neutrophils was found, but not RAGE-/- mice were elevated, but not RAGE-/- mice were found, and not RAGE-/- mice were found. In reaction to AA, RAGE expression stimulated sustained neutrophil accumulation in mice's BALF.

Source link: https://doi.org/10.3389/fimmu.2023.1039997


A new perspective on acoustic rhinometry in terms of standardisation, including the nasal allergen provocation test

It appears that the rhinometric curve points refer to various waves for the nasal cavity accurately reflect the severity of the nasal cavity's mucous membrane's response to an allergen.

Source link: https://doi.org/10.5114/ada.2021.109689


Allergen bronchoprovocation: correlation between FEV1 maximal percent fall and area under the FEV1 curve and impact of allergen on recovery

During allergen bronchoprovocation testing, house dust mite causes greater responses than other allergens. The maximum percent reduction in forced expiratory volume in a second is reported by ABP testing's standard methods, as well as the area under the FEV1 vs time curve. Aims We wanted to determine if the early asthmatic response and the late asthmatic response of FEV1, max and AUC during the early asthmatic warning and the late asthmatic response, and to see if the EAR recovery period for HDM is longer than other allergens compared to other allergens. Max varied between allergens and FEV1, but the LAR AUC and FEV1, max did not differ, but not. Following HDM challenges, there was no evidence of delayed EAR recovery, so HDM did not have a higher response than some other allergens tested.

Source link: https://doi.org/10.1186/s13223-023-00759-6


Effect of gut microbial composition and diversity on major inhaled allergen sensitization and onset of allergic rhinitis

Background: Decreased gut microbiota diversity is associated with gut dysbiosis and other illnesses, including allergic disorders. To significant inhaled allergens, we investigated the connection between gut microbial diversity and sensitization. In addition, the connection of allergic symptom emergence in sensitized people with bacterial composition was investigated. Methods: This report included 1092 local residents who had registered in the Iwaki Health Promotion Project in 2016. Patients with sensitized or unaware of allergen, as well as symptomatic and symptomatic groups were compared between patients sensitized or not exposed to allergen. Compared to the unsensitized group, some u03b1-diversity metrics were dramatically reduced in patients who were sensitive to any/all four allergens. The intestinal microbiota composition and diversity of gut microbiota were similar between the symptomatic and asymptomatic groups. The onset of allergy symptoms may not be directly related to bacterial composition and species. Bacteroidales at the order level may have an effect on sensitization; however, the onset of allergy symptoms was not particularly related to bacterial composition or diversity.

Source link: https://doi.org/10.1016/j.alit.2022.06.005


The Effectiveness of Allergen Immunotherapy in Adult Patients with Atopic Dermatitis Allergic to House Dust Mites

Allergen immunotherapy is not a first-line therapy for atopic dermatitis, but its use has been criticized. AIT's efficacy and safety in adult patients with AD and monosensitization to house dust mites were investigated. For 12 months, patients received Purethal mites containing the extract allergens D. pteronyssinus and D. farinae or a placebo. Conclusions : HDM-AIT's performance effectively treated atopic dermatitis in patients that were strictly dependant on monovalent mite allergy sensitization.

Source link: https://doi.org/10.3390/medicina59010015


Component-Resolved Diagnosis Based on a Recombinant Variant of Mus m 1 Lipocalin Allergen

Given the importance of sequence/structure analysis in protein antigenic reactivity, we compared the sequence of the variant Mus m 1. 0102 to other Mus m 1 allergen members and developed Discotope 2. 0 to predict conformational epitopes based on its 3D-structure. The antigen source for mouse allergy testing is based on serum IgE testing, which uses an epithelial extract as the antigen source. Recombinant Mus m 1. 0102 was a useful tool for investigating fine epitope mapping of specific IgE reactivity to mouse allergy's key allergen.

Source link: https://doi.org/10.3390/ijms24021193


Ragweed Major Allergen Amb a 11 Recombinant Production and Clinical Implications

Ragweed pollen is highly allergenic and causes type I hypersensitivity reactions in the affected populations. ragweed allergy patients were recruited prospectively over a three-year period; a comprehensive questionnaire was administered; and sera were collected and stored. Amb a 11 was produced in parallel with patients' recruitment in parallel. u2019 recruitment In E. coli and Sf9 Spodoptera frugiperda cells, the gene coding for mature protein was introduced. IgE reactivity was tested in 150 patients'u2019 sera for both recombinant allergen forms in ELISA, according to a hRBL hexosaminidase release assay, with 5 positive sera being further tested assay. Compared to nAmb a 1. It is a key IgE-reactive component of ragweed pollen, with high IgE sensitization prevalence in the sample population and allergenicity of the recombinant allergen comparable to Amb a 1.

Source link: https://doi.org/10.3390/biom13010182


Binding to Iron Quercetin Complexes Increases the Antioxidant Capacity of the Major Birch Pollen Allergen Bet v 1 and Reduces Its Allergenicity

Bet vs 1 is the most common allergen in birch pollen, and it has influenced up to 95% of patients sensitivity to birch pollen. We investigated Bet v 1's ability to sequester iron and its immunomodulatory properties on human immune cells as well as its immunomodulatory properties. FeQ2 was estimated in docking estimates and by spectroscopy, with Bet vs. iron quercetin complexes FeQ2 estimated. With the human reporter cell line AZ-AHR, human mast cells and Arylhydrocarbon receptor stimulation were investigated on human mast cells and Arylhydrocarbon receptor activation. Senitized human mast cells degranulation were reduced by up to 80% and impaired degranulation of sensitized human mast cells following binding to FeQ2 masked IgE epitopes and reduced IgE binding up to 80% and impaired degranulation of sensitized human mast cells. The closing down of quercetin, which triggered the anti-inflammatory AhR pathway and increased the labile iron pool of human monocytic cells, was facilitated by Bet vs. Nourishing immune cells with complex iron can also be a promising antigen-independence immunotherapeutic strategy to increase allergy-related immunotherapy's efficacy.

Source link: https://doi.org/10.3390/antiox12010042


Suitability of potyviral recombinant virus-like particles bearing a complete food allergen for immunotherapy vaccines

VLPs derived from Turnip mosaic virus have demonstrated their ability to display molecules of concern on their surface, making them fascinating tools to be investigated in theragnostic studies. Here, we investigate their potential in the treatment of food allergy by genetically coupling TuMV-derived VLPs to Pru p 3, one of the most common allergens in Mediterranean climates. VLPs-Pru p 3 were created by cloning a synthetic gene encoding the TuMV coat protein and Pru p 3 genes, separated by a linker, into a transient high-expression vector and agroinfiltration in Nicotiana benthamiana plants, followed by agroinfiltration. VLP-Complex sublingual administration of mice may be able to reduce some serological markers associated with allergic responses in mice, including anti-Pru p 3 sIgE and sIgG2a. Our findings indicate that in N. benthamiana plants, standard production of allergen-coated TuMV-VLPs is demonstrated.

Source link: https://doi.org/10.3389/fimmu.2022.986823

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions