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Allergen - Crossref

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Last Updated: 28 January 2023

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Human IgE monoclonal antibody recognition of mite allergen Der p 2 defines structural basis of an epitope for IgE cross-linking and anaphylaxis in vivo

IgE bound to its high affinity receptor on mast cells and basophils activates the release of histamine and other allergic mediators. Due to serum IgE's polyclonal composition of serum IgE and the low number of IgE-producing B cells in blood, little is known about the direct allergen-IgE antibody exchange. Here, we introduce the X-ray crystal structure of a house dust mite allergen, Der p 2, in the complex with Fab's isolation of a human IgE monoclonal antibody isolated by hybridoma technology from human B cells from an allergic subject.

Source link: https://doi.org/10.1093/pnasnexus/pgac054


Allerdictor: fast allergen prediction using text classification techniques

During the previous years, several allergen prediction services have been produced. Moreover, the most accurate devices are also slow, because they use protein sequence alignment to create feature vectors for allergen classifiers. Moreover, only web server implementations of the new allergen prediction software are publicly available and do not have the capability of large batch submission. Allerdictor, a fast and accurate sequence-based allergen prediction system that models protein sequences as text documents and uses a support vector machine in text classification for allergen prediction, has been developed by the researchers. Allerdictor predicted allergens with high precision over fast recall at high speeds in multiple highly skewed datasets, according to test findings from several highly skewed databases. 1% of them were allergens, for example, Allerdictor only took u223c6 min on a single core PC to search a whole Swiss-Prot database of u223c540 000 sequences and identified 1% of them as allergens.

Source link: https://doi.org/10.1093/bioinformatics/btu004


RAGE contributes to allergen driven severe neutrophilic airway inflammation via NLRP3 inflammasome activation in mice

Although significant progress has been made in the treatment of asthma, subsets of patients with airway neutrophilia may have more frequent disease and are unlikely to respond to traditional corticosteroid therapy. Methods A mouse model of severe steroid-resistant neutrophilic airway disease was used by a researcher using the common fungal allergen Alternaria alternata to determine the effects of RAGE deposition and pharmacological inhibition of neutrophilic airway inflammation in a mouse model. In wild-type mice, AA exposure increased neutrophil-dominant airway inflammation and elevated BALF levels of Th1/Th17 cytokines, which was dramatically reduced in RAGE -/- mice. Pharmacological inhibition of NLRP3 prevented the effects of AA intake and NLRP3 inflammasome activation, which was RAGE-dependent. In response to AA, time-course studies revealed that RAGE proteins promoted sustained neutrophil accumulation in the BALF of mice's BALF.

Source link: https://doi.org/10.3389/fimmu.2023.1039997


Air pollution in the places of Betula pendula growth and development changes the physicochemical properties and the main allergen content of its pollen

The aim of the study was to determine the effect of air pollutants on the chemical composition of birch pollen and the Bet v1 protein's secondary structures. We have found small variations in the values of parameters describing the photosynthetic light reactions, similar spectra of leaf reflectance, and negligible differences in the discrimination characteristics of the u03b4 13 C carbon isotope were found. Mean Bet v1 concentration in pollen samples obtained in Kraku00f3w was significantly higher than in less polluted locations, on the other hand, while FT-Raman spectra showed the most distinct variations in the wavenumbers characteristic of proteins.

Source link: https://doi.org/10.1371/journal.pone.0279826


SORTALLER: predicting allergens using substantially optimized algorithm on allergen family featured peptides

Abstract Summary: SORTALLER is an online allergen classification system that uses an allergen family's peptide database and standardized BLAST E-values, which determine the featured vectors for support vector machines.

Source link: https://doi.org/10.1093/bioinformatics/bts326


Reconsider the Art of Allergen Immunotherapy

The confirmation of the clinical relevance of IgE findings is important, according to She, who said that molecular IgE analysis aids the diagnostic workup and personalized risk assessment in complex situations. Dramburg said that digital technologies could help physicians make medical decisions at the point-of-care by targeted patient data, guideline- and evidence-based clinical studies, and prospectively collected patient- and sensor-generated data. Patients with allergies react differently to different allergen doses and allergen immunotherapy products, and minor/intermediate allergens are required for optimal clinical safety, in comparison to key allergens. In grass polls, allergens that are relevant for AIT's effectiveness are identified, but for other allergen sources, such as birch and house dust mite, are not yet determined. According to Zieglmayer, the aim should be to establish a match between the patient molecular sensitisation profile and the allergen preparation, and that this can be achieved.

Source link: https://doi.org/10.33590/10179590


Attenuation of allergen-mediated mast cell activation by rosemary extract ( Rosmarinus officinalis L.)

In several cellular contexts in vitro and in vivo, Rosemary extract has been shown to modulate the MAPK and NF-u03baB pathways. However, the effect of RE on mast cell activation and Fc/u03b5RI/c-kit signaling has yet to be explored, and so far we intend to investigate the possibility of RE in modulating mast cell proliferation and Fc/u03b5RI/c-kit signaling, potentially through these key pathways. When treated with 0u201325 b/5g/ml RE, primary murine mast cells were sensitized with anti-TNP IgE and stimulated with cognate allergen under stem cell factor potentiation. While simultaneously reducing NF-u043aB transcription factor occurrence, RE treatment reduced the phosphorylation of p38 and JNK MAPKs. Importantly, RE treatment reduced early phase mast cell degranulation during early phase lymphocyte degranulation, with carnosic acid and carnosol contributing. These results reveal that RE is capable of modulating mast cell functional outcomes, and that further investigation of the underlying mechanisms and its potential therapeutic properties in allergic inflammatory conditions is warranted.

Source link: https://doi.org/10.1002/jlb.3a0320-434r


New Frontiers: Precise Editing of Allergen Genes Using CRISPR

CRISPR makes significant changes in editing quality, throughput, and precision in comparison to previous gene editing techniques. CRISPR has demonstrated success in many clinical areas, including sickle cell disease and u03b2-thalassemia, as well as preliminary knockout studies of allergenic proteins using CRISPR editing, which include preliminary knockout studies. Given the benefits of CRISPR as well as specific DNA markers in the allergen genes, CRISPR gene editing is a viable option for treating allergy, which may lead to significant disease changes.

Source link: https://doi.org/10.3389/falgy.2021.821107


Allergen bronchoprovocation: correlation between FEV1 maximal percent fall and area under the FEV1 curve and impact of allergen on recovery

The maximum percent decrease in forced expiratory volume in one second and the area under the FEV 1 vs time curve are the two most common ABP test results. We wanted to determine if the EAR recovery period for HDM would be longer than other allergens during the early asthmatic response and the late asthmatic response, as well as other allergens. We compared EAR and LAR FEV 1, max, AUC, and absolute difference in FEV 1, max to the most recovered FEV 1, max to the most recovered FEV 1. Max differences between allergens and FEV 1, max differed between allergens, but the LAR AUC and FEV 1, max did not differ, but not. HDM had no evidence of EAR repair after HDM challenges, so HDM did not produce a more significant response relative to all other allergens tested.

Source link: https://doi.org/10.1186/s13223-023-00759-6


Treatment with pollen allergen immunotherapy improves health-related quality of life in children and adolescents: a three-year follow-up-study

Abstract Background The immunological effects of allergen-specific immunotherapy have been well documented, but no research has looked at the long-term effects of pollen subcutaneous immunotherapy on children and adolescents, as well as adults. In a 3-year follow-up, the aim of this research was to determine the effect of pollen SCIT on HRQoL and to determine the connection between HRQoL and symptoms in children and adolescents with allergic rhinoconjunctivitis. For the association of HRQoL and influencing factors, the relationship between HRQoL and influencing factors was investigated over time, using ANOVA and t-test. After one year of SCIT, the number of children with severe or persistent symptoms decreased from 35. 6% to 4. 5%. During the study period, Allergen-specific IgE antibodies decreased, birch from 151. 0 to 76. 8 kU/L, and IgG4 antibodies increased, birch from 151. 0 to 17. 6 g/L, grass from 0. 5 to 14. 3 g/L.

Source link: https://doi.org/10.1186/s13223-023-00756-9

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions