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We found that FK506 reduced inflammation in lung innate cells as well as the induction of TH2 cells, which cause chronic inflammation, using a mouse model of airway inflammation triggered by Papain, a protease allergen, and RNAseq analysis of lung innate cells. Our results support the clinical value of FK506 for the treatment of allergen-induced airway inflammation, as well as establishing the target and mechanism of action.
Source link: https://www.ncbi.nlm.nih.gov/bioproject/785555
In the mediastinal LN to HDM allergens, MARCH1-/- mice exhibit a deficit in TH2 cell growth. Pulmonary DCs that isolate allergens and migrate to mediastinal lymph node have been blamed for triggering TH2 cell polarization. The DCs were divided into two Zbtb46GFP based on the medLNs of CD45. 1/. 2. Primary analysis with this application for the WTDC sample revealed 2,441 cell-barcodes per cell with 7,651 median unique molecular identifiers per cell and 2,004 median genes per cell sequenced to 61. 4% sequencing saturation, with 48,559 mean reads per cell. 681 cell-barcodes with 6,309 median unique transcripts per cell and 1,774 median genes per cell sequenced to 73. 1% sequence saturation with 202,410 mean reads per cell reported in this study. MARCH1-/- DC sample: 681 cell-barcodes with 6,309 median unique transcripts per cell and 1,774 median genes per cell sequenced to 73. 1% sequencing saturation with 202,410 median reads per cell and 1,774 median genome sequenced.
Source link: https://www.ncbi.nlm.nih.gov/bioproject/763224
During the pollen peak season and stratified patients by safe allergy-immunotherapy or untreated, we produced sputum in hay fever patients and healthy controls, as well as healthy controls. During AIT's three-year journey, Secretoglobin1A1 and IL-24 protein levels were measured for Secretoglobin1A1 and IL-24 protein levels in an additional validation cohort at three separate time points. During the course of AIT, the growth of SCGB1A1 in upper airways coincided concomitantly with the reduction of local IL-24 in upper airways. In addition, SCGB1A1 has been identified as a suppressor of epithelial gene expression in the lower airways, and the absence of multiple regulatory pathways on one hand suggests a novel anti-inflammatory mediator of long-term allergen specific therapeutic intervention in the local environment.
Source link: https://www.ncbi.nlm.nih.gov/bioproject/704278
To common allergens; to specific microbial allergens dermatophagose, Dermatophagoides farina, Aspergillus genitus, Dermatophagodes nigricans, and Fusarium proliferatum are among the allergens used in this study. Der p 1 being mite serine proteinase; to birch pollen allergens ; to common wormwood allergens ophthal o.
Source link: https://www.ncbi.nlm.nih.gov/bioproject/692941
The combination of IL-6 and soluble IL-6R can cause IL-6 trans-signaling in cells such as epithelial cells and fibroblasts, which are both measurable GP130, but not produce enough IL-6R. In vitro, we performed next-generation RNA sequencing to determine the expression of the IL-6R subunits by eosinophils stimulated by IL-3 in vitro, in addition to protein analysis of bronchoalveolar lavages by ELISA and flow cytometry. Overall plan: Gene expression profiles of blood eosinophils from three subjects with allergy and mild asthma were cultured in RPMI and 10% serum with or without IL-3 for 4 h and 20 hours.
Source link: https://www.ncbi.nlm.nih.gov/bioproject/670482
In the post-deployment scenario, soldiers are then exposed to common household allergens, which leads to persistent respiratory disease outbreaks. For 12 days in a row, C57BL/6 mice were exposed to filter air or aerosolized APM for six hours per day using a whole body exposure system, followed by intranasal PBS or HDM allergen challenges. Mice were euthanized to obtain bronchoalveolar lavage cells for single cell RNA sequencing using the 10x Genomics Platform and analysis of inflammation and airway hyperresponsiveness, one day after the last HDM challenge. Compared to filtered air controls, APM pre-exposure and HDM challenge followed by a PM recall lead to significant increases in total respiratory system resistance in comparison to filtered air controls. We found that inhalation of desert PM while on deployment to Afghanistan could make airways more sensitive to allergen exposure after returning home.
Source link: https://www.ncbi.nlm.nih.gov/bioproject/649562
Patients with asthma patients with asthma are particularly susceptible to elevated bloodway inflammation caused by CD4+ helper T cells and regulatory T cells that respond to common allergens. We investigated the single-cell transcriptome of 50,000 house dust mite allergy and Treg cells from asthmatics with HDM allergy and asthmatic with and without HDM allergy in asthma and asthma. In asthmatics, the number of interleukin -9 expressing HDM-reactive TH cells is higher than in asthmatics with HDM allergy, but non-asthmatics with HDM allergy are more common, and they exhibit enhanced pathogenic capabilities. In asthmatics without HDM allergy asthmatics and others with HDM allergy are more likely to see Th and Treg cells expressing the interferon-response signature in asthmatics without HDM allergy than in asthmatics with HDM allergy.
Source link: https://www.ncbi.nlm.nih.gov/bioproject/609655
Patients with asthma are highly affected by CD4+ helper T cells and regulatory T cells that respond to common allergens, which play a significant role in driving and dampening airway inflammation. We analyzed the single-cell transcriptome of 50,000 house dust mite allergy and Treg cells from asthmatics with HDM allergy and non-asthmatics with and without HDM allergy to better understand the variety of these T cell subsets in allergy and asthma. The number of interleukin -9 cells that express HDM-reactive TH cells in asthmatics is higher in asthmatics than those that do not have HDM allergy, and they exhibit enhanced pathogenic characteristics. Compared to asthmatics with HDM allergy and those with HDM allergy, asthmatics without HDM-reactive Th and Treg cells with the interferon-response signature are present in asthmatics without HDM allergy but not in asthmatics with HDM allergy.
Source link: https://www.ncbi.nlm.nih.gov/bioproject/609120
We investigated the effect of house dust mite and lipopolysaccharide challenge on AM from patients with mild asthma in this research. asthma patients have asthma patients who have shown previously that intrabronchial HDM/LPS challenges cause a mixture of eosinophilic and neutrophil airways inflammation. In the contralateral lung segment, AM was harvested from the bronchoalveolar fluid of 8 asthma patients who were treated with either saline in one lung segment or HDM/LPS. Following the HDM/LPS challenge, we performed RNA sequencing and discovered over 1000 highly expressed genes. This is the first investigation to investigate gene expression profiles in AM from asthma patients treated with HDM and LPS in vivo, to the best of our knowledge. We discovered different transcriptional profiles in AM as a result of local exposure to HDM and LPS using a well-controlled study design that allows for paired analysis of saline and HDM/LPS results in the same patient.
Source link: https://www.ncbi.nlm.nih.gov/bioproject/604044
Humans are able to digest and absorb Goat's milk, as compared to Cow's milk. Feces of offspring were found before allergen sensitization and two days after i. t. At Day 0: Comparative ratio of Firmicutes to Bacteroidetes, water-fed group had a higher F/B ratio than GM-fed and CM-fed group. After HDM allergen sensitization and challenge, there was a drastic rise in F/B ratio in water-fed mice, but there was no change in F/B ratio in GM-fed and CM-fed mice. Overall analysis: A total sample of 44 mice was examined.
Source link: https://www.ncbi.nlm.nih.gov/bioproject/602719
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