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Agar - Europe PMC

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Last Updated: 16 August 2022

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Hydrothermal treatments – A quick and efficient alternative for agar extraction from Gelidium sesquipedale

Gelidium sesquipedale, a highly versatile hydrocolloid with gelling capability, is renowned for its high-quality agar, a highly sought hydrocolloid with gelling capability, which is an industrially useful property strongly dependent on its design and composition. paragae's agar culture. The present study was designed to determine the use of autohydrolysis for extracting agar from this marine macroalgae to close the gap. 2. 1 u00b1 0. 1 The results revealed that the ideal processing temperature for agar recovery was 2. 1 u00b1 0. 1. This condition not only helps preserve the extraction yield, but it also results in a gelling strength of 758 g/cmu00b2, all while allowing for a drastic processing time reduction. On the other hand, 3,6-anhydro-l-galactose content and overall structure appeared to be inherent to the biomass and not affected by extraction conditions. When properly tuned, autohydrolysis can be used to quickly and accurately produce high-quality food-safe agars.

Source link: https://europepmc.org/article/MED/IND607814132


Increasing xanthan gum content could enhance the performance of agar/konjac glucomannan-based system

An increasing amount of XG in the final ternary solution with strong KGM-XG bond, as shown by rheological and thermal tests, could entanglement in the resulting ternary solution with strong KGM-XG binding. For example, a ternary system with a 16% XG content displayed simultaneous rises in the zero-shear viscosity, sol-gel transition point, and gel hardness. In addition, the AKX-16% composite had lower crystallinity and improved hydrogen bonding and tensile strength resulting from gel dehydration.

Source link: https://europepmc.org/article/MED/IND607791328


Gelatin/agar-based multifunctional film integrated with copper-doped zinc oxide nanoparticles and clove essential oil Pickering emulsion for enhancing the shelf life of pork meat

The clove oil Pickering emulsion was created from nanocellulose as a solid base. On ZnO@Cu and PEC addition, a 0% reduction of L. monocytogenes and a u223c 50% decrease in the E. coli population was also demonstrated on film, with strong antimicrobial activity on the gelatin/agar films. In addition, the functional films' antioxidant value was twice higher than neat films due to PEC's presence. The pork belly meat was wrapped in a multifunctional film before vacuum packing, which helped significantly reduce the pork belly meat's total bacterial count and lipid oxidation.

Source link: https://europepmc.org/article/MED/IND607834988


A double layer agar plate method results in an improvement for enumerating Vibrio vulnificus and Vibrio parahaemolyticus exposed to nutrient deficiency and refrigeration temperature

In V. vulnificus incubated aerobically, a positive correlation between sodium pyruvate and the establishment of platable colony-forming capacity was found. Using tryptic soy agar containing 3% salt TSASAS was overlaid with thiosulfate-bile salts-sucrose agar TCBS, a higher platable colony number for Vibrio spp.

Source link: https://europepmc.org/article/MED/IND607814591


Analysis of biofilm expansion rate on agar substrates with different stiffness under limited nutrition

We investigate Bacillus subtilis biofilm growth on substrates with different agar levels, as well as how to identify biofilms' area, morphology data, and fluorescence intensity by using optical imaging techniques in this research. The interaction between biofilm and each agar substrate rises during first three days of growth, which makes the biofilm expansion rate decreases as wrinkle pattern IV comes up. The biofilm has the highest expansion rate on 2. 0 % agar concentration, which has the largest wrinkle distance in wrinkle pattern IV, reducing energy consumption after three days, after wrinkle pattern IV emerged.

Source link: https://europepmc.org/article/PPR/PPR530298


A high-fidelity agar-based phantom for ultrasound-guided brain biopsy simulation: A novel training prototype with visual feedback.

Aim The aim of this paper was to develop and evaluate Ultrasonography-guided brain biopsy training. Through pre and post training trials, the impact of multimodal training is determined. The participants were divided into familiar-14 and unfamiliar-11 according to their ability with USG-based procedures. In translucent glass bowls, opaque top and transparent middle layers were constructed; each having 12 embedded targets; each having 12 embedded targets. Participants in two controlled trials of USG guided biopsy with aluminum foil covering the glass bowls, avoiding visual cues. Feedback forms confirmed the effect of the self-training session on subjective confidence levels with the procedure. Pre-training Familiars had better results in EfB, Ef, Er, and PS compared to strangers. Conclusions Simulation and phantom based models can never replace education by supervised skill development in-vivo, but our model complements education by facilitating technical skill acquisition, particularly for beginners in USG guided Brain Biopsy.

Source link: https://europepmc.org/article/MED/35961586


Colistin resistance screening by 3 μg/ml colistin agar in Carbapenemase-producing Enterobacterales.

Background Background: The use of colistin in therapeutic regimens in low- and middle-income countries is widespread, treating infections caused by Carbapenemase-producing Enterobacterales due to a lack of access to the recently approved antibiotics. In the present study, we report the use of agar dilution with a low colistin content of 3 u03bc/ml to distinguish isolates with colistin resistance in CPE isolated from clinical samples. In 168 isolates of CPE isolated from clinical samples in Guayaquil, Ecuador, 168 isolates of CPE obtained from clinical samples were tested by the Clinical Laboratory Standards Institute and a dilution agar with a colistin concentration of 3 u03bcg/ml. Using CLSI breakpoints as a comparison, Broth microdilution was considered our gold standard in the industry. Conclusion We recommend the use of dilution agar with a colistin content of 3 u03bc/ml as a reliable screening device for screening colistin resistance in low- and middle-income countries to measure resistance and perform epidemiological studies.

Source link: https://europepmc.org/article/MED/35944176


Potential Contribution to Dietary Reference Intake (DRI) of Agar Extracted from Gelidium latifolium (Gelidiaceae, Rhodophyta) as a Mineral Source.

Using the inductively coupled plasma mass spectroscopy technique, Ni, Cu, Mo, and Se were determined in agar obtained seasonally from Gelidium latifolium off the coast of Black Sea. The agar's potential contribution to the dietary reference intakes was investigated in the Institute of Medicine and the Official Journal of the European Union, as well as the Basic Nutritional Requirements Guideline published by the Institute of Medicine and the European Union's Economic Journal. The results indicate that agar extracted from G. latifolium could be used as a food supplement to help ensure that DRIs have sufficient essential minerals and trace elements. Extractive agar values for extracted agar were between 0. 36 and 40. 54, so they could be able to reduce hypertension, preeclampsia, and heart disease in human beings. Extract agar from G. latifolium red seaweed was of high quality and safety, and could be used in the field of diet, according to the agar.

Source link: https://europepmc.org/article/MED/35943707


Empirical estimation of skin resistance to water loss in amphibians: agar evaluation as a non-resistance model to evaporation.

The sum of the boundary layer and the skin resistance determines the absolute resistance of amphibians to evaporative water loss in amphibians. Under the assumption that the agar surface creates no barrier to evaporation, the use of agar models has become the standard method to determine rb.

Source link: https://europepmc.org/article/MED/35818822


Pressurized hot water-assisted recovery of crude residual agar from a never-dried algae industry waste stream: A Box-Behnken design approach

Since an initial industrial extraction was carried out, it was investigated whether using pressurized hot water extraction to valorize the remaining crude agar in Gelidium sesquipedale's waste stream. To fit all of the experimental responses to a second-order polynomial equation that confirmed the approach's suitability, a multiple regression statistical framework was used. A high extraction yield of 17. 0 percent was predicted for a 120 °C, low pressure of 3. 28 bars, and an extended extraction time of 150 min along with a 3% algae content. Between a minimum of 25 g/cm/cm/cm/u00b2 and a maximum of 350 g/cm/cmu00b2 is the maximum amount of g/cm/cm/u00b2; a maximum of 350 g/cm/cm/u00b2u00b2. In addition to the quadratic effect of the solid concentration, the key parameters affecting the change in the sulfate content in the recovered agar appeared to be the temperature and the algae concentration.

Source link: https://europepmc.org/article/MED/IND607707747

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions