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Agar - DOAJ

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Last Updated: 16 July 2022

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Calcium sulfate beads made with antibacterial essential oil-water emulsions exhibit growth inhibition against Staphylococcus aureus in agar pour plates.

Calcium sulfate bone void filler beads are completely absorbable in the body and are often used in difficult orthopedic disease cases to give a relatively high dose of antibiotics locally to the body site over time. However, the antibiotic resistance epidemic and/or inability to treat chronic biofilm infections remain to be a significant and increasing health threat to society. In this study, we tested the likelihood that plant essential oils with anti-staphylococcal characteristics could slow the growth of Staphylococcus autus in agar pour plates treated with calcium sulfate beads. According to the null hypothesis, 55/57 PEOs had significant growth inhibitory activity, and 41/57 PEOs had similar behavior to or higher than a vancomycin minimum inhibitory control. PEOs tests in PEOs revealed significant growth inhibition when encased in S. aureus-seeded agar, relative to a null hypothesis of six millimeters compared to a null hypothesis of six millimeters.

Source link: https://doi.org/10.1371/journal.pone.0271209


Comparison of Legionella Biofilm Formations at Three Different Temperatures in Liquid Flow, in Static Liquid and on Agar Plate

The three-dimensional structures of 8-days biofilm were investigated under nutrient water flow conditions at 37°C, 40°C, and 42°C. Also, bacteria lengths were compared among the biofilms in liquid flow, those in static liquid, and those on BCYE-α agar plate; agar plate. Our experiments on L. pneumophila biofilm formation will help us get a better understanding of the vivacious mode of life of L. pneumophila.

Source link: https://doi.org/10.1299/jbse.6.160


Investigation of Tigecycline Susceptibility of Multidrug-Resistant Acinetobacter Baumannii Isolates by Disc Diffusion, Agar Gradient and Broth Microdilution Tests

Because of the high prevalence of antibiotic resistance in Acinetobacter baumannii isolates, the use of tigecycline is becoming more significant. Multidrug-resistant A. baumannii isolates were isolated from various tissue and fluid samples of patients admitted to or treated at various departments and tested in the Laboratory of Microbiology, Duzce University Medical Faculty between January 2013 and December 2015. The Tigecycline kinetic resistance in multidrug-resistant A. baumannii isolates was determined using the disc diffusion test, agar gradient test, and a gold standard test [broth microdilution test]. The study found that A. baumannii isolates resistant to multiple medications. The 89, 4 and 1 A. baumannii isolates were identified as tigecycline-sensitive, intermediate, and resistant isolates, respectively, using the BMT method. BMT's Tigecycline resistance as measured by BMT showed no change between 2013 and 2015.

Source link: https://doaj.org/article/3b2e1908205540c2838a84bd7e67efb6


Evaluation of Colistin Broth Disk Elution and Colistin Agar Test: A study from Tertiary Care Hospital, South India

Polymyxin E and polymyxin B are the last two options for treating CRE-related infections. Hence, the present study was conducted to determine the results of the colistin broth disk elution and colistin agar test in comparison to the reference broth microdilution method. Since increasing colistin resistance, it is crucial to report colistin MIC with a documented technique, so we want to recommend CAT testing for routine MIC reporting of colistin because it is a simple test.

Source link: https://doi.org/https://doi.org/10.22207/JPAM.16.2.08


Evaluation of selective mitis salivarius agar for the isolation of Streptococcus mutans and its resistance pattern in Bangladesh

Since Streptococcus mutans is the most common cause of dental caries, appropriate laboratory equipment is required for the proper detection and treatment of this bacterium. The purpose of this study was to determine the mitis salivarius agar's agar compared to traditional blood agar media for detection of bacterium. This research was conducted in the Rajshahi Medical College, Rajshahi, Bangladesh's Department of Microbiology from April 2017 to December 2017. The sample, dental swab was obtained from 200 people aged between 6-18 years who underwent dental caries and lived in Rajshahi district. Antimicrobial susceptibility testing was also done in this study. In MSA than BAM, higher growth was recorded in MSA rather than BAM. Penicillin G was the most sensitive, followed by Azithromycin Azithromycin. The study findings may help to raise the detection of mutans and its pattern for appropriate care in Bangladesh's improved dental health.

Source link: https://doi.org/10.5455/jabet.2022.d115


Clinico-mycological Study of Otomycosis Comparing the Cavity Slide Technique and the Conventional Agar Block Slide Culture

Introduction: Otomycosis is a sub-acute or chronic superficial fungal infection of the external auditory canal that occurs mainly in tropics and subtropics. Following a primary bacterial infection, some species of Aspergillus and Candida commonly invade the ear canal, but other fungal pathogens are also associated with otomycosis. The fungal etiology in otomycosis is often used to delineate the fungal etiology in otomycosis, but the morphology is not particularly clear when compared to the cavity slide culture, which holds the morphology well. Aim: To isolate various fungal agents involved in otomycosis and then compare the cavity slide culture approach with the traditional agar block slide method for identification. All specimens were inoculated on Sabouraud dextrose agar, Blood agar, and Mac Conkey agar, but only those with fungal growth were further processed. For the detection of fungi, fungi was identified by a comparison of agar block slide culture and cavity slide culture. The most common fungal isolate was Aspergillus flavus followed by A. n. . . . , A. fumigatus, Penicillium, Candida albicans, and Candida albicans out of 120 fungal isolates. The cavity slide culture method was found to be more effective at proper visualisation and preservation of morphology of fungi. As the morphology is better preserved and appreciated in it, the results are available in a short period of time, the outcomes should be used regularly for the visualisation and identification of fungi.

Source link: https://doi.org/10.7860/JCDR/2019/41417.13058


Antimycobacterial screening of selected medicinal plants against Mycobacterium tuberculosis H37Rv using agar dilution method and the microplate resazurin assay

The recent rise in the number of multi-drug resistant clinical isolates of Mycobacterium tuberculosis has caused urgent need for the discovery and production of new anti-TB drugs. Two in vitro assays, namely agar dilution assay and microplate resazurin assay against M. tuberculosis H37Rv were tested against M. tuberculosis H37Rv at different concentrations of prepared extracts, were tested against M. tuberculosis H37Rv. Results: The results of this research revealed that aqueous extracts from four selected medicinal plants Ocimum sanctum, Adhatoda vasica, Leptadenia reticulata, and Cocculus hirsutus had minimum inhibitory concentrations against M. tuberculosis in 500 ml, 250 bcg/ml, respectively, and C. sanctum, A. reticulata, L. Conclusion: This report helps society discover new anti-TB drugs that improve anti-TB activity with reduced or no side effects.

Source link: https://doi.org/10.4103/ijmy.ijmy_60_20


THE USE OF STEVIA AND MONK FRUIT SWEETENERS FOR SUGAR REPLACEMENT IN GREEN TEA AGAR JELLIES

Both natural non-nutritive sweeteners, stevia and monk fruit were tested at 25%, 50%, 75%, and a 100% sugar replacement based on the equivalent sucrose sweetness. The more sugar substitute reduced the total soluble solids, but the gel firmness and the lightness were enhanced. Against the whole sugar jellies, the syneresis reduced in reduced-sugar jellies made with stevia and monk fruit. The stevia and monk fruit replacements in agar jellies' taste and flavor profiles were affected by the stevia and monk fruit substitution. Over 50% and 75% sugar replacement, either stevia or monk fruit, were preferred over 25% and 100% replacement over 25% and 100% replacement.

Source link: https://doi.org/10.33512/fsj.v3i2.12404


Development of New Biodegradable Agar-Alginate Membranes for Food Packaging

The paper discusses the possibility of replacing the polyethylene packaging from food products with biodegradable packaging made from biopolymers. Two lines of membranes were created, one with ascorbic acid in 150 mL filmogenic solution and the other with calcium chloride in a 150 mL filmogenic solution, to improve the coating material's properties. By SEM and roughness analysis, the mixture components were found to be uniform in the presence of AA and CACl 2 and roughness analysis. The findings show that agar/alginate/glycerol membranes with AA and CaCl 2 may be used in an active food packaging system.

Source link: https://doi.org/10.3390/membranes12060576

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions