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Agar - Crossref

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Last Updated: 16 August 2022

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Evaluation of white blood cell concentration, plasma fibrinogen concentration, and an agar gel immunodiffusion test for early identification of foals with Rhodococcus equi pneumonia

An AGID assay performed an anti-R equi antibody at 4-week intervals and took samples from each foal at 4-week intervals for WBC and plasma fibrinogen concentrations testing and at 2-week intervals for determination of anti-R equi antibody by an AGID assay. The use of receiver operating characteristic curve analysis was used to determine the diagnostic results of WBC and fibrinogen concentrations. u2014Diagnostic results of WBC concentrations were noticeably higher than that of fibrinogen concentrations, despite fibrinogen concentrations. Typical values for WBC concentrations at a cutoff of 13,000 cells/u00b5L were 95. 2 and 61. 2%, respectively; with a sensitivity cutoff of 15,000 cells/u00b5L of 90. 8%, sensitivity was 96. 6 percent and specificity was 90. 8%. When a positive test result was used as the cutoff, the AGID assay's sensitivity was 66. 5 percent and specificity was 50. 8 percent. The conclusion and clinical relevance of a u2014Monitoring WBC test can be used early in the identification of infected foals on farms with a high incidence of R equi pneumonia.

Source link: https://doi.org/10.2460/javma.2003.222.775


Efficacy of Off-Label Anti-Amoebic Agents to Suppress Trophozoite Formation of Acanthamoeba spp. on Non-Nutrient Agar Escherichia Coli Plates

Acanthamoeba keratitis is a rare infectious disease that is associated with a high risk of blindness among the infected patient and for which no appropriate therapy exists as yet. Of the eight off-label anti-amoebic drugs tested, only PVPI enabled for complete reduction of trophozoite formation by drug-challenged cysts for all four Acanthamoeba isolates in all five biological replicates. With some, but not all, validated Acanthamoeba isolates, the test Acanthamoeba isolates' exhales, but not all, failed to have a significant effect on the excystation capacities of the tested Acanthamoeba isolates in the majority, if not all, of our repeats. According to our results, pre-testing of the AK isolate with the non-nutrient agar E. coli plate assay against the anti-amoebic drug intended for therapy should be done to ensure that the selected drug is cysticidal for the Acanthamoeba isolate.

Source link: https://doi.org/10.3390/microorganisms10081642


The Effect of Ethanol Extract of Yam Bulbs (Pachyrhizus Erosus L Urb) on the Growth of Staphylococcus Aureus Bacteria Agar Diffusion Method

The Bengkuang Plant is a plant that can be used as herbal medicine. Using the agar diffusion method, the aim of this study was to determine the ethanol tuber extract concentration on the development of Staphylococcus aureus bacteria. The sample of this study is ethanol extracted from bamboo tuber ethanol with concentrations of 10%, 20%, 40%, 60%, 70%, 80%, and 90%. Based on the results of the study, the determination of the inhibitory zone of Staphylococcus aureus bacteria was found to average 6,00 mm at concentrations of 80% and 9. 33 mm at concentrations of 80% and 9. 33 mm at concentrations of 80%, 7. 33 mm at concentrations of 80%, 7. 67 mm at concentrations of 90% at a concentration of 90%.

Source link: https://doi.org/10.30602/jtkb.v2i1.33


In Vitro Study of the Interaction of Gentamicin with Ceftriaxone and Azithromycin against Neisseria gonorrhoeae Using Agar Dilution Method

In this research, the sensitivity of gentamicin isolated in three Chinese provinces and the association of gentamicin, azithromycin, and ceftriaxone isolates were investigated. The results of this research were also used to determine the efficacy of gentamicin as a combination therapeutic drug. On N. gonorrhoe isolates, the minimum inhibitory concentrations of ceftriaxone, azithromycin, and gentamicin were determined by using agar dilution technique. In this research, the majority of the N. gonorrhoeae isolates tested in this study demonstrated a certain degree of sensitivity to gentamicin.

Source link: https://doi.org/10.3390/antibiotics11081083


Analysis of biofilm expansion rate on agar substrates with different stiffness under limited nutrition

The interaction stress between biofilm and each agar substrate increases in the first three days of u2019 growth, which leads to decreases in the biofilm expansion rate as well as wrinkle pattern IV rises. The biofilm has a higher expansion rate on 2. 0wt% agar concentration, which has the largest wrinkle distance in wrinkle pattern IV, lowering energy consumption after three days, after wrinkle pattern IV appears.

Source link: https://doi.org/10.21203/rs.3.rs-1869265/v1


Agar-Based Disc Diffusion Assay for Susceptibility Testing of Dermatophytes in the Rajshahi Region

Background: Dermatophytosis is one of the most common public health disorders worldwide and is the most common skin disease in Bangladesh. dermatophytes were identified by a microscopic examination using a 10% KOH mount, mycological history, and species identification by lactophenol cotton blue mount from culture. Trichophyton rubrum was the most common dermatophyte species among 171 samples, followed by T. mentagrophyte, at 15, E. floccosum were 15, and M. canis were 03. Voriconazole and Itraconazole were the more effective drugs. Despite several treatment options being available for cutaneous fungal infections, cutaneous fungal infections remain an epidemic problem. The disc diffusion technique is a simple and cost-effective method for determining antifungal susceptibility of dermatophytes.

Source link: https://doi.org/10.3329/taj.v35i1.61164

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions