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Acid Production - Europe PMC

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Last Updated: 04 December 2022

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Development of a nutrient-demand model for King salmon (Oncorhynchus tshawytscha) to predict dietary macronutrient and amino acid requirements across the grow-out production phase

This study's aim was to produce a nutrient-demand model for King salmon based on a factorial nutrient demand approach. Using laboratory results to determine the thermal response boundaries and benchmarked against commercial farm-data, growth potential was determined. Based on the analysis of whole-body samples from 110 g/fish to 4786 g/fish, Algorithms of body composition for this species were determined across the size ranges for this species. Applying the best protein concept to protein gain and maintenance, as well as estimates of individual amino acid usage efficiency variations, were forecast. According to the model, a higher dietary intake of protein, relative to other salmonid diets, might have potential benefits for King salmon.

Source link: https://europepmc.org/article/MED/IND607833378


Improved hydrogenation process for margarine production with no trans fatty acid formation by non-thermal plasma with needle-in-tube configuration

Refined palm olein was used as raw material with operating conditions at ambient pressure, room temperature, and no catalyst. After plasma treatment was evaluated, results of hydrogen gas concentration, input power, gas flow rate, discharge gap size, and reaction time were all investigated. With an increase in saturated fatty acid composition from 60. 82 to 31. 39 percent, the lowest iodine value was achieved under the condition of 0. 5 percent flow rate, 15 percent gap size, and 15 h reaction time, the lowest iodine value was reduced from 60. 82 to 36. 6 percent. Generated hydrogen free radicals were supposed to perform hydrogenation by reacting with carbon-carbon double bonds, converting poly- and monounsaturated fatty acids into saturated ones.

Source link: https://europepmc.org/article/MED/IND607791287


Engineering Gluconobacter cerinus CGMCC 1.110 for direct 2-keto-L-gulonic acid production.

The potential SNDH/SDH gene cluster in the strain Gluconobacter cerinus CGMCC 1. 110 was mined by genome analysis, and its function in converting L-sorbose to 2-KLG was established in the present study. The expression level of SNDH/SDH had a major effect on the titer of 2-KLG, according to Proseomic analysis, and fermentation findings revealed that SDH was the rate-limiting enzyme. After fermentation optimization, a standardized metabolic engineering process, which was theoretically suitable for raising the titer of many products involving membrane-bound dehydrogenase from Gluconobacter, was then applied to a 5-L bioreactor, increasing the 2-KLG titer in G. cerinus CGMCC 1. 110 from undetectable to 51. 9 g/L in a 5-L bioreactor. u2022 (U2022) THE potential SNDH/SDH gene cluster in G. cerinus CGMCC 1. 110 was mined.

Source link: https://europepmc.org/article/MED/36445390


Metabolic and Microbial Community Engineering for Four-Carbon Dicarboxylic Acid Production from CO 2 -Derived Glycogen in the Cyanobacterium Synechocystis sp. PCC6803.

In a dim anoxic environment, Cyanobacteria excretes significant amounts of C4 dicarboxylic acids by glycogen fermentation. The increase of metabolic flux in the Cyanobacterium Synechocystis sp's reductive TCA branch is responsible for the increase of metabolic flux in the Cyanobacterium Synechocystis sp. Peptck expression in PEPck concurrent with Ppc resulted in a rise in C4 dicarboxylic acids by autofermentation. PEPck, according to a rise in carbon flux from hexose and pentose phosphates to the TCA reductive branch, according to a Metabolome study, contributing to an increase in carbon flux from hexose and pentose phosphates to a rise in carbon flux from hexose and pentose phosphate phosphates to the TCA reductive branch. We investigated the effect of corn-steep liquor as a nutritional supplement on C4 dicarboxylic acid production in the reductive TCA branch to raise the metabolic flux in the reductive TCA sector. After 72 hours of cultivation, high-density cell culture of cells lacking the acetate kinase gene led to the highest production of malate, fumarate, and succinate. Microbial community engineering in the present microbial community engineering is useful for succinate production under anoxic environment.

Source link: https://europepmc.org/article/MED/36445137


Cost Reduction in the Production of Spirulina Biomass and Biomolecules from Indole-3-Acetic Acid Supplementation in Different Growth Phases.

Therefore, finding more effective cultivation practices in biomass growing and economic viability is therefore essential. In various phases of Spirulina sp. , this work sought to minimize the production costs of biomass and biomolecules using phytohormone indole-3-acetic acid. In this sense, this work was designed to minimize the production costs of biomass and biomolecules using phytohormone indole-3-acetic acid. The addition of indole-3-acetic acid in half of the microbiolga's growth deceleration phase showed a cost decrease of 27 percent, 34%, and 75% for biomass, proteins, and carbohydrates, respectively. This report is the starting point for introducing phytohormone supplementation in industrial microalgal cultures.

Source link: https://europepmc.org/article/MED/36441405


Concurrent production of ferulic acid and glucose from wheat bran by catalysis of a putative bifunctional enzyme.

This work also established the enzymatic properties of the bifunctional enzymes made using microcrystalline cellulose and p-nitrogenyl butyrate as substrates, and found 40 u00b0C at 40 °C, the optimum temperature for their enzymatic reactions. Endoglucanase and carboxylesterase were both 6. 0 for endoglucanase and 7. 0 for carboxylesterase in dual function. The bifunctional enzyme also demonstrated enzymatic activity on the natural biomass by supplying up to 3. 94 mg of glucose and 49. 4 bcg of ferulic acid from 20 mg of destarched wheat bran (20 mg).

Source link: https://europepmc.org/article/MED/36442604


Thermophilic caproic acid production from grass juice by sugar-based chain elongation.

Most chain elongation studies have concentrated on mesophilic chains, with only two recent studies indicating the possibility of thermophilic chain elongation, but a systematic review of its mechanisms is lacking. Here, we investigated thermophilic chain elongation from grass juice to find the effect of key operational parameters on the process's results, and to identify the key microbial genera and their role in the process. During batch experiments, the substrate for caproic acid production was glucose and xylose, while lactic acid led to the production of only butyric acid. The final average caproic acid concentration increased by 6% by glucose addition during continuous operation, contributing to a microbial community dominated by Caproiciproducens and up 76 percent to 6. 0 g/L, which represented 32 percent of total carboxylic acids.

Source link: https://europepmc.org/article/MED/36436634


A novel co-production of cadaverine and succinic acid based on a thermal switch system in recombinant Escherichia coli.

Due to reduced contamination and environmental friendliness, the less pollution and environmental friendliness of cadaverine and succinic acid production has been more appealing. However, the accumulation of cadaverine can cause significant damage to cells, resulting in cell death and cadaverine production. Results The fermentation process was divided into two phases, one for cell growth and lysine production and the other for cadaverine and succinic acid synthesis. In a cadaverine plant strain, the genes of ldhA and ackA were deleted to create succinic acid pathway. The optimal temperature for the second stage was 39 u2103. The fermentation conditions were found to be 33 u2103 for the first stage, which was 39 u2103. Susinic acid was also obtained with a yield of 0. 19 g/g glucose on 28. 39 g/L. Conclusions The succinic acid metabolic pathway was developed into a cadaverine producer strain in order to obtain succinic acid and cadaverine co-production.

Source link: https://europepmc.org/article/MED/36419122


Optimization of L-malic acid production from acetate with Aspergillus oryzae DSM 1863 using a pH-coupled feeding strategy.

We therefore wanted to increase L-malate production from acetate with A. oryzae by using a pH-coupled feeding method. The malic acid concentration was up 5. 3 percent compared to the batch process without pH control, resulting in a maximum titer of 29. 53 g/L after 264 h, but it was not possible to keep both the pH and substrate concentration constant during this fermentation. Despite carbon availability, a potential product inhibition was discovered in shake flask cultivations, since cessation of malic acid production was discovered in later fermentation stages. Even at high product concentrations, A. oryzae is capable of organic acid production in these experiments, which include malate and succinate, a key by-product during malic acid production, was discovered that in these experiments, malate and succinate, a common by-product during malic acid manufacturing, were added at up to 50 g/L.

Source link: https://europepmc.org/article/MED/36419102


Poly-γ-Glutamic Acid Production by Engineering a DegU Quorum-Sensing Circuit in Bacillus subtilis .

Due to its unique characteristics of biocompatibility, biodegradability, water solubility, and viscosity, a natural biological macromolecule, u03b3-polyglutamic acid plays a key role in medicine, food, and cosmetic industries. Although many steps have been tried to raise the yield of u03b3-PGA in Bacillus subtilis, the effectiveness of these common approaches is not high because the high viscosity affects cell growth. However, a flexible regulation based on quorum sensing has been widely used as a keystone for fine-tuning gene expression in reaction to cell density changes without adding expensive inducers. We investigated the DegU-based gene expression regulation scheme in B. subtilis 168, first in this study. We developed a promoter library of various abilities, selected good promoters from the library, and performed mutation testing on the selected promoters and degU region. Cell growth and effective synthesis of the target product can be dynamically balanced by the QS system.

Source link: https://europepmc.org/article/MED/36416371

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions