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Acid Production - DOAJ

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Last Updated: 04 January 2023

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Engineering of a newly isolated Bacillus tequilensis BL01 for poly-γ-glutamic acid production from citric acid

Abstract Background Poly phosphorus acid, a form of polyethylene, is a promising biopolymer suitable for a variety of uses. Susrose and 2SO4 at 37°C, respectively, were the best sources of carbon and nitrogen, according to respectively. Then, the titer of u03b3-PGA, saw the increase in titer 0. 3 g/L by co-overexpression of the citZ and pyk genes in the mutant strain, as shown by titer 0. 3 g/L. In addition, the titer of u03b3-PGA 0. 8 g/L with a yield of 1. 50 g of u03b3-PGA/g of citric acid in fed-batch fermentation reached 25. 3 g/L/h and a yield of 1. 50 g/L. This report, it should be noted, allows the synthesis of low and high molecular weight of u03b3-PGA by BL01 and the engineering strain. Conclusion The use of recently published methods to raise u03b3-PGA production for the new strain B. tequilensis BL01 is shown. Compared to the normal strain in the flask and 5 L fermenter, the titer of u03b3-PGA increased 2. 17 fold and 1. 32 percent.

Source link: https://doi.org/10.1186/s12934-022-01994-z


Optimization of succinic acid production from xylose mother liquor (XML) by Actinobacillus succinogenes using response surface methodology

Abstract In the present study, conditions for succinic acid production by Actinobacillus succinogenes GXAS137 were optimized using xylose mother liquor as culture medium. Firstly, single-u2013factor experiments were conducted to determine the basal culture medium for SA fermentation. XML, corn steep liquor powder powder, and MgCO3 were used to screen out three primary variables, including XML, corn steep liquor powder powder, and MgCO3, which ultimately increased SA yields from the original nine factors. These results show that XML could be a substitute substrate for A. succinogenes GXAS137's cost-effective production of SA.

Source link: https://doi.org/10.1080/13102818.2022.2095303


Alleviating glucose repression and enhancing respiratory capacity to increase itaconic acid production

In Saccharomyces cerevisiae, the Crabtree effect's ethanol and acetic acid can be used for itaconic acid production. When glucose was used as the substrate, however, both the IA synthetization and oxidative phosphorylation pathways were hampered by glucose repression. This research was designed to raise IA titer by increasing gene expressions related to glucose derepression without impairing yeast growth on glucose. We discovered that several signaling pathways regulating glucose regulation were more effective than full pathway overexpression, increasing IA production and respiratory capacity.

Source link: https://doi.org/10.1016/j.synbio.2022.12.007


Identification of a major facilitator superfamily protein that is beneficial to L-lactic acid production by Bacillus coagulans at low pH

Abstract Background: Abstract Background: Product inhibition is one of the most common problems in lactic acid fermentation. Its mutant strain B. coagulans Na-2 of B. coagulans Na-2 has greater resistance to sodium lactate stress, but the exact mechanism has yet to be determined. B. coagulans Na-2 was sequenced by whole-genome sequencing, and one mutant gene mfs coding for the key facilitator superfamily protein was revealed by comparative genome analysis. 2. 1 and 0. 45 higher than those of the control strain during pH-controlled fermentation at 5. 0. Conclusions The MFS protein found here will increase the ability of B. coagulans to survive acidic environments and produce more L-LA at low pH.

Source link: https://doi.org/10.1186/s12866-022-02736-2


Potential Use of Cow Manure for Poly(Lactic Acid) Production

Cow manure is an abundant waste and poses a challenge for recycling. Hence, cow manure contains a substantial amount of lignocellulose. Cow manure can be valorized by using such lignocellulosic fractions as the raw material of several fermentative processes. This review details the most recent methods for obtaining lactic acid from lignocellulosic biomass in the Lab. Herein, lignocellulosic fraction extraction, sugar conversion, pretreatment, hydrolysis, purification, and polymerization are among the key process parameters required to obtain PLA from lignocellulose.

Source link: https://doi.org/10.3390/su142416753


Fumaric Acid Production by R. arrhizus NRRL 1526 Using Apple Pomace Enzymatic Hydrolysates: Kinetic Modelling

Fumaric acid is one of the most popular biorefinery platform chemicals, with fruit residues being a particularly useful raw material for its production in second generation biorefineries. Apple pomace is a common leftover from the apple juice industry, with apple being the second largest fruit crop in the world, with production increasing from 46 to 86 Mtons in the 1994-2021 period. Apple pomace is made every year in the United States with a worldwide apple juice production of more than 4. 5 million tonnes. Following the fermentation process, APH fermentation was continued and improved using various nitrogen initial amounts, resulting in yields similar to those obtained with synthetic media. Kinetic modeling was used to determine, explain, and report the chemical properties and trends of key components in the fermentation broth as a result of the bioprocess time, providing a robust reaction method and a non-structured, non-segregated kinetic model based on it.

Source link: https://doi.org/10.3390/pr10122624


The Superiority of Bacillus megaterium over Escherichia coli as a Recombinant Bacterial Host for Hyaluronic Acid Production

Recombinant HA production using other microbial platforms has been gaining interest in preventing potential toxin contamination associated with the product's streptococcal fermentation. The zoopedemicus was ligated into plasmid pMM1522, resulting in pMM1522 hasA, which was released into E. coli Rosetta-2 and B. megaterium. The resulting plasmid pP T7 hasABC and hasABC genes were ligated into plasmid pP T7 and various E. coli host strains were then transformed with the resulting plasmids pP T7 hasABC and hasABC deoxygene, respectively. For E. coli Rosetta-gamiBpLysS is a product of E. coli The original Rosetta-gamiBpLysS production in fantastic broth medium was 500 u00b1 11. 4 mg/L. With pP T7 hasABC and pP T7 hasABC, we also converted B. megaterium protoplasts carrying T7-RNAP. The HA product's infrared spectroscopy spectra were similar to those obtained from commercially available standard polymers. Also, scanning electron microscopic examination revealed extensive HA capsules in E. coli Rosetta-gamiBpLysS, although no HA capsules were manufactured by B. megaterium. Conclusions: Our findings showed that Gram-positive bacteria are superior host strains for recombinant HA production over their Gram-negative counters, according to our results. Both the titers and molecular weight of HA obtained by B. megaterium were significantly higher than those obtained by other E. coli host strains used in this study.

Source link: https://doi.org/10.3390/microorganisms10122347


Citric Acid Production by Yarrowia lipolytica NRRL Y-1094: Optimization of pH, Fermentation Time and Glucose Concentration Using Response Surface Methodology

Yarrowia lipolytica strains were tested for acid-production capability in this research, and the maximum zone-area was established by Y. lipolytica NRRL Y-1094. The CA production by Y. lipolytica NRRL Y-1094 was optimized using response surface methods and considering three parameters, including initial pH-value, fermentation time, and initial glucose-concentration. According to estimated regression-coefficients for CA concentrations, the fermentation time had the most effect on CA production, followed by the substrate concentration and initial pH-level, respectively.

Source link: https://doi.org/10.3390/fermentation8120731


Using Machine Learning Methods to Predict the ß-Poly (L-Malic Acid) Production by Different Substrates Addition and Secondary Indexes in Strain Aureobasidium melanogenum

We added potassium acetate, corn steep liquor, MgSO 4, MnSO 4, MnSO 4, vitamin B1, vitamin B1, vitamin B1, vitamin B1, and nicotinamide as the fermentation substrate to the basic fermentation medium based on a randomly generated random matrix that represented the added value in this research. PMLA production was negatively related to corn steep liquor and betaine, but it was positively related to potassium acetate, according to the studies. Based on the PMLA's production, the convolutional neural network was used to forecast PMLA production by fermentation medium images. The methods and results reported in this review provided new insight into estimating different substrate concentrations on PMLA production and demonstrating the possibility of using the convolutional neural network model in the PMLA fermentation process.

Source link: https://doi.org/10.3390/fermentation8120729


Use of Pressurized and Airlift Bioreactors for Citric Acid Production by Yarrowia lipolytica from Crude Glycerol

Citric acid production is generally carried out in a squeous medium in stirred tank reactors, where oxygen solubility is low and oxygen demand of microbial cultures is high. Citric acid production by Yarrowia lipolytica W29 from crude glycerol in batch cultures was carried out in two non-conventional bioreactors traditionally associated with high mass transfer rates: a pressurized STR and an airlift bioreactor. Both bioreactor types can be used in the citric acid production process, with alternative methods of increasing OTR improvement rather than increasing mechanical stirring power input, resulting in significant operating savings costs.

Source link: https://doi.org/10.3390/fermentation8120700

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions