Advanced searches left 3/3

Acid Nucleic Hybridization - Europe PMC

Summarized by Plex Scholar
Last Updated: 16 May 2022

* If you want to update the article please login/register

Development of fluorescence-xBased nucleic acid blot hybridization method using Cy5.5 labeled DNA probes.

Here, we used a NIR fluorescent cyanine dye Cy5. 5 to tag DNA probes for nucleic acid hybridization by nucleic acid blot hybridization. Both Southern blot and Northern blot used cellulolytic bacterium Ruminiclostridium cellulolyticum as a model, demonstrating the specificity and sensitivity of fluorescent DNA probes. The fabrication and determination of Cy5. 5-labeled probes are more reliable, more accurate, and versatile, compared to other non-radioactive probes, proving that the Cy5. 5-labeled probes are more effective for nucleic acid hybridization in comparison to bioimaging.

Source link: https://europepmc.org/article/MED/35504367


Binary detection of protein and nucleic acid enabled cancer diagnosis through branched hybridization chain reaction.

The early diagnosis and clinical theranostics has been made possible by the production of isothermal bio-analyzers for enhanced detection of multi-factor diseases such as cancer biomarkers. In the downstream process, in the presence of hemin, split sequences of a DNAzyme were introduced in close proximity to aid in the solution's color change to a green hue. respectively, the assay enabled sensitive detection of miR-21 and PDGF-BB-related cancer biomarkers with LOD values of 10 pM and 40 nM by ingenious exerting multi-level amplification.

Source link: https://europepmc.org/article/MED/35414385

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions